Browse > Article
http://dx.doi.org/10.3746/jkfn.2005.34.7.937

Antioxidant and Anticancer Activities of Extracts from Styela plicata  

Kim, Jin-Ju (Division of Food Science and Biotechnology, Kyungnam University)
Kim, Sun-Jung (Division of Food Science and Biotechnology, Kyungnam University)
Kim, Sun-Hee (Division of Food Science and Biotechnology, Kyungnam University)
Park, Hae-Ryong (Division of Food Science and Biotechnology, Kyungnam University)
Lee, Seung-Cheol (Division of Food Science and Biotechnology, Kyungnam University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.34, no.7, 2005 , pp. 937-941 More about this Journal
Abstract
The antioxidant and anticancer activities of Styela plicata extracts were evaluated. When extracts were prepared with fresh Styela plicata (FR), extraction yield was in the order of methanol > ethanol = acetone > water among treated solvents. However, the extraction order was methanol > water > ethanol > acetate in freeze dried Styela plicata (FD). Radical scavenging activity was the highest in acetone extracts $(37.39\%)$ from FR, while in ethanol extracts $(78.40\%)$ from FD. Reducing power of FR was the greatest in methanol extracts (1.076), and that of FD in ethanol extracts (1.360). The acetone extracts from FD showed significant anticancer activity when revealed with human colon cancer cell line HT-29. These results indicated that extraction yields and properties of extracts from Styela plicata were variable depending on solvent and/or physicochemical state, and appropriate extraction process could provide some valuable bioactive materials from Styela plicata.
Keywords
Styela plicata; antioxidant; anticancer; freeze-drying;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Jo YG. 1978. The sterol composition of Styela clava. Kor Fish Soc 11: 97-101
2 Ahn SH. 2003. Extraction of glycosaminoglycans from Styela clava tunic. Biotechnol Bioproc Eng 18: 180-185
3 Lehrer RI. 2001. Clavanins and styelins, alpha-helical antimicrobial peptides from the hemocytes of Styela clava, Adv Exp Med Biol 484: 71-76   DOI
4 Lee IH, Zhao C, Nguyen T, Menzel L, Waring AJ, Sherman MA, Lehrer RI. 2001. Clavaspirin, an antibacterial and haemolytic peptide from Styela clava. J Pept Res 58: 445-456   DOI   ScienceOn
5 Taylor SW, Craig AG, Fischer WH, Park M, Lehrer RI. 2000. Styelin D, an extensively modified antimicrobial peptide from ascidian hemocytes. J Bioi Chem 275: 38417-38426   DOI   ScienceOn
6 Oyaizu M. 1986. Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 44: 307-315   DOI
7 National Statistical Office. 2001. Annual Report on the Cause of Death Statistics. Seoul, Korea
8 Goodman GY, Yen YP, Cox TC, Crowlny J. 1987. Effect of verapamilon in vitro cytotoxicin and vinblastin in human tumor cell. Cancer Res 47: 2295-2311
9 Blake D, Winyard PG. 1995. Immunopharmacology of free radical species. Academic Prss, San Diego
10 Fish B. 1984. Clinical trials for the evaluation of cancer therapy. Cancer Res 54: 609-615
11 SAS Institute. 1995. SAS/STAT User's Guide. SAS Institute Inc., Cary, NC
12 Yokozawa T, Chen CP, Dong E, Tanaka T, Nonaka GI, Nishioka I. 1998. Study on the inhibitory effect of tannins and flavonoids against the 1,1 -diphenyl-2-picrylhydrazyl radical. Biochem Pharm 56: 213-222   DOI   ScienceOn
13 Diplock AT. 1997. Will the good fairies please prove to us that vitamin E lessens human degenerative disease? Free Rad Res 27: 511-532
14 Jung BM, Lim SS, Park YJ, Bae SJ. 2005. Inhibitory effects on cell survival and quinone reductase induced activity of Aster yomena fractions on human cancer cells. J Korean Soc Food Sci Nutr 34: 8-12   DOI   ScienceOn
15 Lopaczynski W, Zeissel SH. 2001. Antioxidant, programmed cell death, and cancer. Nutr Res 21: 295-307   DOI   ScienceOn
16 Choe WK, Park JH, Kim SH, Lee DY, Lee YC. 1999. Antitumor effects of green tea catechin on different cancer cells. Korean J Nutr 32: 838-843
17 Choi BD, Kang SJ, Choi YJ, Youm MG, Lee KH. 1994. Utilization of ascidian (Halocynthia roretzi) tunic. 3. Carotenoid compositions of ascidian tunic. Bull Korean Fish Soc 27: 344-350
18 Lee KH, Park CS, Hong BI, Jung BC, Cho HS, Jea YG. 1995. Seasonal variations of nutrients in warty sea squirt (Styela clava). J Korean Soc Food Nutr 24: 268-273
19 Cook NC, Samman S. 1996. Flavonoids chemistry, mechanism, cardioprotective effects and dietary source. Nutr Biochem 7: 66-76   DOI   ScienceOn
20 Namiki M. 1990. Antioxidants/antimutagens in food. Crit Rev Food Sci Nutr 29: 273-300   DOI   ScienceOn
21 Gerlier D, Thomasset N. 1986. Use of MTT colorimetric assay to measure cell activation. J Immun Meth 94: 57-63   DOI   ScienceOn
22 Park KY, Moon SH, Rhee SH, Baek KY, Lim SY. 1995. Effect of tannin from persimmon leaves on the growth inhibition and the synthesis of mRNA of type IV collagen in AZ-521 human gastric cancer cells. Environm Muta Carcino 15: 32-37
23 Lee SC, Kim JH, Jeong SM, Kim DR, Ha JU, Nam KC, Ahn DU. 2003. Effect of far-infrared radiation on the antioxidant activity of rice hulls. J Agric Food Chem 51: 4400-4403   DOI   ScienceOn
24 Cotelle N, Moreau S, Bernier JL, Catteau JP, Henichart JP. 1991. Antioxidant properties of natural hydroquinones from the marine colonial tunicate Aplidium californicurn. Free Radic BioI Med 11: 63-68   DOI   ScienceOn
25 Park JC. 1996. Screening of marine natural products on inhibitory effect of the formation of lipid peroxidation. Kor J Pharmacogen 27: 117-122
26 Bondent V, Brand-Williams W, Bereset C. 1997. Kinetics and mechanism of antioxidant activity using the DPPH free radical methods. Lebensm- Wiss Technol 30: 609-615   DOI   ScienceOn
27 Sato A, Shindo T, Kasanuki N, Hasegawa K. 1989. Antioxidant metabolites from the tunicate Amaroucium multiplicatum. J Nat Prod 52: 975-981   DOI
28 El-Agarney A, Lowe GM, McGarvey DJ, Mortensen A, Phillip DM, Truscott TG, Young AJ. 2004. Carotenoid radical chemistry and antioxidant/pro-oxidant properties. Arch Biochem Biophys 430: 37-48   DOI   ScienceOn
29 Mier S, Kanner J. Akiri B, Hadas SP. 1995. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J Agric Food Chem 43: 1813-1819   DOI   ScienceOn
30 Menzel LP, Lee IH, Sjostrand B, Lehrer RI. 2002. Immunolocalization of clavanins in Styela clava hemocytes, Dev Comp Immunol 26: 505-515   DOI   ScienceOn
31 Matsuno T, Ookubo M, Komori T. 1985. Carotenoids of tunicates. III. The structural elucidation of two new marine carotenoids, amarouciaxanthin A and B. J Nat Prod 48: 606-613   DOI
32 Kang TB, Liang NC. 1997. Studies on the inhibitory effects of quercetin on the growth of HL -60 leukemia cells. Biochem Pharm 54: 1013-1018   DOI   ScienceOn