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http://dx.doi.org/10.3746/jkfn.2005.34.5.605

Effect of a Herb Mixture (HIM-I) on the Protection of the Hematopoietic-Immune System and Self-renewal Tissues against Radiation Damage  

Park, Hae-Ran (Radiation Food and Biotechnology Team, Korea Atomic Energy Research Institute)
Kim, Sung-Ho (College of Veterinal Medicine, Chonnam National University)
Yee, Sung-Tae (Dept. of Biology, Sunchon National University)
Byun, Myung-Woo (Radiation Food and Biotechnology Team, Korea Atomic Energy Research Institute)
Jo, Sung-Kee (Radiation Food and Biotechnology Team, Korea Atomic Energy Research Institute)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.34, no.5, 2005 , pp. 605-612 More about this Journal
Abstract
In previous studies, we evaluated the effect of the 6 energy-tonic or blood-building prescriptions of traditional oriental medicine, and observed that Si-Wu-Tang and Bu-Zhong-Yi-Qi-Tang showed high activity in the protection of the gastrointestinal and hematopoietic organs in irradiated mice. But any of these prescriptions did not show a high activity in the activation of the immune cells. We performed this study to design an herb mixture which protects the self-renewal tissues and also promotes recovery of the immune system against radiation damage. In order to meet all the requirements, we designed a new mixture of 3 edible herbs listed in Korean Food Code. The mixture of Angelim gigas radix, Cnidium officinale rhizoma and Paeonia japonica radix was decocted with hot water, and the activities of the water extract (HIM-I) were evaluated. HIM-1 stimulated the immune cells in a much higher extent than the traditional prescriptions, and promoted dramatically the growth of bone marrow stem cells in vitro. Also, HIM-1 protected digestive and hematopoietic organs against radiation as effectively as the 2 prescriptions, Si-Wu-Tang and Bu-Zhong-Yi-Qi-Tang. On the other hand, it showed high in vitro antioxidative activity that might be considered as a mechanism of the protective effects against radiation. Although the detailed mechanisms of those effects remain to be elucidated, these results indicated that HIM-I might be a useful agent for protection and recovery of body from various risk factor as well as radiation, especially since it is a relatively nontoxic natural product.
Keywords
herb mixture; radiation protective; hematopoietic; immune; jejunal crypt; spleen;
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1 Ikeda S, Kaneko M, Kumazawa Y, Nishimura C. 1990. Protective activities of a Chinese medicine, hochu-ekki-to, to impairment of hematopoietic organs and to microbial infection. Yakugaku Zasshi 110: 682-687   DOI
2 IAEA. 1978. Safety series No. 47: Manual on Early Medical Treatment of Possible Radiation Injury. IAEA, Vienna. p 74
3 NCP. 1980. Report No. 65: Management of Persons Accidentally Contaminated with Radionuclides. IAEA, Vienna. p77
4 Hall EJ. 1988. Radiobiology for the Radiologist. 3rd ed. J.B. Lippincott Company, Philadelphia. p 365-376
5 Patt H, Tyree M, Straube RL. 1949. Cystein protects against x-irradiation. Science 110: 213-214   DOI
6 Washburn LC, Carlton JE, Hayes RL. 1974. Distribution of WR-2721 in normal and malignant tissue of mice and rats bearing solid tumors: dependence on tumor type, drug dose and species. Radiat Res 59: 483-575
7 Sweeney TR. 1979. A survey of compounds from the antiradiation drug development program of the U.S. army medical research & development command. Walter Reed Army Institute of Research, Washington, DC. p 55
8 Cairnie AB. 1983. Adverse effect of radioprotector WR2721. Radiat Res 94: 221-226   DOI   ScienceOn
9 Neta R, Douches S, Oppenheim JJ. 1986. Interleukin 1 is a radioprotector. J Immunol 136: 2483-2485
10 Kim SH, Lee SE, Oh H, Kim SR. 2002. The radioprotective effects of Bu-Zhong -Yi-Oi-Tang: A prescription of traditional Chinese medicine. Am J Chin Med 30: 127-137   DOI   ScienceOn
11 Hartzman RJ, Bach ML, Bach FH. 1972. Precipitation of radioactivity labeled samples, a semi-automatic multiplesample processor. Cell Immunol 4: 182-186   DOI   ScienceOn
12 Milas L, Hunter N, Reid BO, Thames Jr HD. 1982. Protective effects of S-2-(3-aminopropylamino) ethylphosphorothioic acid against radiation damage of normal tissues and a fibrosarcoma in mice. Cancer Res 42: 1888-1987
13 Milas L, Murray D, Brock WA, Meyn RE. 1988. Radioprotectors in tumor radiotherapy: Factors and settings determining therapeutic ratio. Pharmacol Ther 39: 179- 189   DOI   ScienceOn
14 Miyanomae T, Frindel E. 1988. Radioprotection of hemopoiesis conferred by Acanthopanax senticosus Harms (Shigoka) administered before or after irradiation. Exp Hematol 16: 801-806
15 Ohta S, Sakurai N, Sato Y, Inoue T, Shinoda M. 1990. Studies on chemical protectors against radiation. XXX. Radioprotective substances of Cnidii rhizoma. Yakugaku Zassh 110: 746-754   DOI
16 Hsu HY, Lian SL, Lin CC. 1990. Radioprotective effect of Ganoderma lucidum (Leyss. ex. Fr.) Karst after X -ray irradiation in mice. Am J Chin Med 18: 61-69   DOI   ScienceOn
17 Quan HX, Li HS. 1994. Effects of radix Astragali on hemopoiesis in irradiated mice. Chung Kuo Chung Yao Tsa Chih 19: 741-743
18 Yonezawa M, Katoh N, Takeda A. 1981. Restoration of radiation injury by ginseng. II. Some properties of the radioprotective substances. J Radiat Res Tokyo 22: 336-343   DOI   ScienceOn
19 Zhang JS, Sigdestad CP, Gemmell MA, Grdina DJ. 1987. Modification of radiation response in mice by fractionated extracts of Panax ginseng. J Radiat Res Tokyo 112: 156-163   DOI   ScienceOn
20 Ohnishi Y, Yasumizu R, Fan HX, Liu J, Takao-Liu F, Komatsu Y, Hosoya E, Good RA, Ikehara S. 1990. Effects of juzen-taiho-toh (TJ-48), a traditional Oriental medicine, on hematopoietic recovery from radiation injury in mice. Exp Hematol 18: 18-22
21 Hsu HY, Hau DM, Lin CC. 1993. Effects of kuei-pi-tang on cellular immunocompetence of gamma-irradiated mice. Am J Chin Med 21: 151-158   DOI   ScienceOn
22 Hsu HY, Ho YH, Lin CC. 1996. Protection of mouse bone marrow by Si-Wu-Tang against whole body irradiation. J Ethnopharmacol 52: 113-117   DOI   ScienceOn
23 Hsu HY, Yang JJ, Lian SL, Ho YH, Lin CC. 1996. Recovery of the hematopoietic system by Si- Jun-Zi-Tang in whole body irradiated mice. J Ethnopharmacol 54: 69-75   DOI   ScienceOn
24 Halliwell B, Gutteridge JM. 1999. Free radicals in biology and medicine. 3rd ed. Oxford University Press, New York. p 604-607
25 Lee SE, Oh H, Yang JA, Chung CY, Jang JS, Jo SK, Byun MW, Kim SH. 1999. The radioprotective effects Sam-RyungBaek-Chul-San (San-Ling-Bai-Shu-San) as a prescription of traditional Chinese medicine in irradiated mice. J Korean Soc Food Sci Nutr 28: 444-451
26 Kim SH, Lee SE, Oh H, Yang JA, Chung CY, Jang JS, Yu YB, Jo SK. 1999. The radioprotective effects of Kuei-PiTang as a prescription of traditional Chinese medicine in mice. J Korean Soc Food Sci Nutr 28: 698-704
27 Jo SK, Yu YB, Oh H, Kim SR, Kim SH. 2000. The effects of Shi-Ouan-Dai-Bu-Tang and ingredients on the survival of jejunal crypt cells and hematopoietic cells in irradiated mice. J Korean Soc Food Sci Nutr 29: 93-98
28 Bidlack WR, Tappel AL. 1973. Damage to microsomal membrane by lipid peroxidation. Lipids 8: 177-182   DOI   ScienceOn
29 Wijsman JH, Jonker RR, Keijzer R, Van Velde CJH, Corneliess CJ, Van Dierendonck JH. 1993. A new method to detect apoptosis in paraffin section: In situ end-labeling of fragmented DNA. J Histochem Cytochem 41: 7-12   DOI   ScienceOn
30 Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 26: 1199-1200
31 Till JE, McCulloch EA. 1980. Hemopoietic stem cell differentiation. Biochim Biophys Acta 605: 431-459
32 Metcalf D. 1989. The molecular control of cell division, differentiation commitment and maturation in haemopoietic cells. Nature 229: 27-30
33 Lee SE, Oh H, Yang JA, Jo SK, Byun MW, Yee ST, Kim SH. 1999. Radioprotective effects of two traditional Chinese medicine prescription: Si-Wu-Tang and Si-Juri-Zi-Tang. Am J Chin Med 27: 387-396   DOI   ScienceOn
34 Fujii Y, Imamura M, Han M, Hashino S, Zhu X, Kobayashi H, Imai K, Kasai M, Sakurada K, Miyazaki T. 1994. Recipient-mediated effect of a traditional Chinese herbal medicine, ren-shen-yang-rong-tang (japanese name: nunjin-youei-to), on hematopoietic recovery following lethal irradiation and syngeneic bone marrow transplantation. Int J Immunopharmacol 16: 615-622   DOI   ScienceOn
35 Potten CS. 1995. Interleukin-11 protects the clogenic stem cell in murine small-intestinal crypts from impairment of their reproductive capacity by radiation. Int J Cancer 62: 356-361   DOI   ScienceOn
36 Kim SH, Jeong KS, Ryu SY, Kim TH. 1998. Panax ginseng prevents apoptosis in hair follicles and accelerates recovery of hair medullary cells in irradiated mice. In Vivo 12: 219-222
37 Neta R. 1988. Role of cytokines in radioprotection. Pharmacol Ther 39: 261-266   DOI   ScienceOn
38 MacVittie TJ, Monroy RL, Patchen ML, Souza LM. 1990. Therapeutic use of recombinant human G-CSF (rhG-CSF) in a canine model of sublethal and lethal whole body irradiation. Int J Radiat Biol 57: 723-736   DOI   ScienceOn
39 Patchen ML, Macvittie TJ. 1983. Dose-dependent responses of murine pluripotent stem cells and myeloid and erythroid progenitor cells following administration of the immunomodulating agent glucan. Immunopharmacology 5: 303-313   DOI   ScienceOn
40 Kim SH, Cho CK, Yoo SY, Koh KH, Yun HG, Kim TH. 1993. In vivo radioprotective activity of Panax ginseng and diethyldithiocarbamate. In Vivo 7: 467-470