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http://dx.doi.org/10.3746/jkfn.2005.34.1.099

Effect of β-Carotene and Vitamin C on Chlorophyll-Induced Photooxidation  

Ryu, Seung-Hee (인제대학교 식품과학연구소, 바이오헬스소재 연구센터 및 인제대학교 식품생명과학부)
Lee, Hye-Suk (인제대학교 식품과학연구소, 바이오헬스소재 연구센터 및 인제대학교 식품생명과학부)
Lee, Young-Soon (경희대학교 식품영양학과)
Kwon, Tai-Wan (인제대학교 식품과학연구소, 바이오헬스소재 연구센터 및 인제대학교 식품생명과학부)
Song, Young-Sun (인제대학교 식품과학연구소, 바이오헬스소재 연구센터 및 인제대학교 식품생명과학부)
Moon, Gap-Soon (인제대학교 식품과학연구소, 바이오헬스소재 연구센터 및 인제대학교 식품생명과학부)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.34, no.1, 2005 , pp. 99-106 More about this Journal
Abstract
Skin is continously exposed to ultraviolet (UV) radiation, the major cause of skin disorders including skin aging. Chlorophylls were well known as photosensitizer initiating subsequent chemical reactions such as photooxidative deterioration of cellular structures. This experiment was designed to elucidate the effects of $\beta$-carotene and ascorbic acid with chlorophylls on UVB-induced photooxidation in linoleic acid emulsion model system and skin homogenate of ICR mouse. In linoleic acid emulsion model system, the addition of chlorophyll and $\beta$-carotene accelerated the photooxidation, while high concentration of ascorbic acid prevented. The combination of chlorophylls, $\beta$-carotene and ascorbic acid, which concentrations are simplified from mustard leaf kimchi, prevented UVB-induced photooxidation. Although single treatment of $\beta$-caretene accelerated photooxidaiton, $\beta$-caretene acted as antioxidant in the combination with ascorbic acid. Similarly the addition of individual chlorophylls and $\beta$-carotene accelerated the UVB-induced photooxidation in skin homogenate of ICR mouse. 50 ppm of ascorbic acid did not show the any preventive effect, however 500 ppm of ascorbic acid effectively prevented the oxidation. Photooxidation was prevented in the combination of chlorophylls and $\beta$-carotene with 500 ppm of ascorbic acid and concentration rate of ascorbic acid plays an important role in the prevention of UVB-induced photooxidation.
Keywords
ultraviolet B; photooxidation; chlorophylls; $\beta$-carotene; ascorbic acid;
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