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http://dx.doi.org/10.3746/jkfn.2004.33.9.1584

Determination of Betaine from Saliconia herbacea L.  

Lee, Chang-Ho (Korea Food Research Institute)
Kim, In-Ho (Korea Food Research Institute)
Kim, Young-Eon (Korea Food Research Institute)
Oh, Se-Wook (Korea Food Research Institute)
Lee, Ho-Jun (Korea Food Research Institute)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.33, no.9, 2004 , pp. 1584-1587 More about this Journal
Abstract
The betaine content of Saliconia herbacea L. was determined by reverse-phase high performance chromatog-raphy on a C_(18) column. A 50% methanol extract was passed through a anion exchanger Ambelite IRA 400 (quaternary ammonium type, OH-) column and a strong cation exchanger Ambelite IR 120 (sulfonic acid type, $H^+$) column to remove amino acids, zwitter ions which are interfere with betaine analysis. The betaine extract was derivatized with 18-crown-6-ether and 4-bromophenacyl bromide (PBPB) for UV-labelling. Betaine in Saliconia herbacea L. was analysed on a mobile phase contained 13 mM sodium heptane sulfonic acid and 5 mM $Na_2SO_4$ in deionized water by isocratic elution for 30 min. The recovery ratio of betaine from Saliconia herbacea L. extract was 83.6%. The mean betaine value for Saliconia herbacea L. determined by the described method is 4.85 mg/mL with a standard deviation of 0.127.
Keywords
Saliconia herbacea L.; betaine; HPLC; halophyte; osmolyte;
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Times Cited By KSCI : 1  (Citation Analysis)
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