Browse > Article
http://dx.doi.org/10.3746/jkfn.2004.33.8.1348

Increase of Conjugated Linoleic Acid (CLA) Contents in Milk by Fermentation with Bifidobacteria Isolated from Korean Infants  

Lee, Hyo-Ku (Dept. of Food Science and Biotechnology, Kongju National University)
Kwon, Yung-Tae (Dept. of Food Science and Biotechnology, Kongju National University)
Kang, Hye-Soon (Dept. of Food and Nutrition, College of Health Sciences, Korea University)
Yoon, Chil-Surk (Live Max Co.)
Jeong, Jae-Hong (Dept. of Hotel Culinary Arts, Ansan Technical College)
Kim, In-Hwan (Dept. of Food and Nutrition, College of Health Sciences, Korea University)
Chung, Soo-Hyun (Dept. of Food and Nutrition, College of Health Sciences, Korea University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.33, no.8, 2004 , pp. 1348-1352 More about this Journal
Abstract
More than 200 Bifidobacterium sp. originated from human intestine were investigated for their ability to produce conjugated linoleic acid (CLA). Of the Bifidobacteria tested, 1 of culture type strain and 12 isolated strains from Korean infants showed CLA producing ability. cis-9, trans-11 octadecadienoic acid presented more than 90% of the total CLA isomers produced by the Bifidobacteria. CLA content in fermented milk by Bifidobacterium sp. KHU 141 increased by 39.6 mg/l00 g, which showed the potential use for producing fermented milk containing high content of CLA. In fermented milk, little changes showed in lauric acid, myristric acid, palmitic acid, oleic acid, and linolenic acid contents, whereas the content of linoleic acid (LA) decreased and the content of CLA increased. Bifidobacterium sp. KHU 141 converted 86.0% and 84.8% of LA consumed to CLA for 24 hr and 48 hr fermentation, respectively. Prolonging incubation from 24 to 48 hours did not appear to enhance CLA formation and CLA producing ability was stable whether bottle, test tube, or fermenter was used for making fermented milk by Bifidobacterium sp. KHU 141.
Keywords
conjugated linoleic acid (CLA); linoleic acid (LA); Bifidobacteria; infants; fermented milk;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Ip C, Angioni E, Carta G, Thompson HJ, Barbano D, Bauman D. 1999. Conjugated linoleic acid enriched butter fat alters mammary gland morphoenesis and reduces cancer risk in rats. J Nutr 129: 2135-2142   DOI
2 Ha YL, Grimm NK, Pariza MW. 1987. Anticarcinogens from fried ground beef; heat-altered derivatives of linoleic acid. Carcinogenesis 8: 1881-1887   DOI   ScienceOn
3 Cook ME, Miller CC, Park Y, Pariza MW. 1993. Immune modulation by altered nutrient metabolism control of immune induced growth depression. Poult Sci 72: 1301-1315   DOI   ScienceOn
4 Miriam LK, Julie RB. 1998. Dietary fatty acid sources affect conjugated linoleic acid concentrations in milk from lactating dairy cows. J Nutr 128: 881-885   DOI
5 Ha YL, Park GB, Kang WS. 1998. Role of conjugated linoleic acid (CLA) as a new functional substance for agricultural and marine products. Proceedings of Int Agr Res Util. Symposium for 50th anniversary GSNU 49-55
6 Kepler CR, Hirons KP, McNeill JJ, Tove SB. 1966. Intermediates and products of the biohydrogenation of linoleic acid by Butyrivibrio fibrisolvens. J Biol Chem 241: 1350- 1354
7 Eyssen H, Vewhulst A. 1984. Biotransformation of linoleic acid and bile acids by Eubacterium lentum. Appl Environ Microbiol 47: 39-45
8 Ham JS, In YM, Jeong SG, Kim JG, Lee EH, Kim HS, Yoon SK, Lee BH. 2002. Screening of conjugated linoleic acid producing lactic acid bacteria from fecal samples of healthy babies. Asian-Aust J Anim Sci 15: 1031-1035   DOI
9 Lee HK, Kwon YT, Kang HS, Yoon CS, Jeong JH, Kim HK, Kim IH, Chung SH. 2004. Conjugated linoleic acid (CLA) contents in commercial yoghurts and production of CLA by commercial dairy starter cultures. J Korean Soc Food Sci Nutr 33: 1343-1347   DOI   ScienceOn
10 Bligh EG, Dyer WJ. 1959. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911- 917   DOI
11 Kim IH, Yoon CS, Lee KW. 2001. Transesterification of conjugated linoleic acid and tricaprylin by lipases in organic solvents. Food Research International 34: 301-306   DOI   ScienceOn
12 Park YK, Albright J, Liu W, Storkson JM, Cook ME, Pariza MW. 1997. Effect of conjugated linoleic acid on body composition in mice. Lipids 32: 853-858   DOI   ScienceOn
13 McCarty MF. 2000. Activation of PPAR gamma may mediate a portion of the anticancer activity of conjugated linoleic acid. Med Hypotheses 55: 187-188   DOI   ScienceOn
14 Chung SH, Kim IH, Kim YJ, Yoon CS. 2001. Increase of conjugated linoleic acid in fermented milk by lactic cultures. AOCS 92nd Annual Meeting & Expo Abstracts S28
15 Chin SF, Liu W, Storkson JM, Ha YL, Pariza MW. 1992. Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. J Food Comp Anal 5: 185-197   DOI
16 Jiang J, Bjorck L, Fonden R. 1998. Production of conjugated linoleic acid by dairy starter cultures. J Appl Microbiol 85: 95-108   DOI   ScienceOn
17 Lin TY, Lin CW, Lee CH. 1999. Conjugated linoleic acid concentration as affected by lactic cultures and added linoleic acid. Food Chemistry 67: 1-5   DOI   ScienceOn
18 Kishino S, Ogawa J, Omura Y, Mastumura K, Shimizu S. 2002. Conjugated linoleic acid production from linoleic acid by lactic acid bacteria. JAOCS 79: 159-163   DOI   ScienceOn
19 Alonso L, Cuesta EP, Gilliland SE. 2003. Production of free conjugated linoleic acid by Lactobacillus acidophilus and Lactobacillus casei of human intestinal origin. J Dairy Sci 86: 1941-1946   DOI   ScienceOn