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http://dx.doi.org/10.3746/jkfn.2004.33.8.1237

Anti-proliferative Effects of Water Extract of Agaricus blazei Murill in Human Lung Cancer Cell Line A549  

Choi, Woo-Young (동의대학교 한의과대학 생화학교실, 부산대학교 생물학과)
Park, Cheol (동의대학교 한의과대학 생화학교실)
Lee, Jae-Yun ((주)청원농산)
Kim, Gi-Young (부산대학교 의과대학 미생물학 및 면역학교실)
Park, Yeong-Min (부산대학교 의과대학 미생물학 및 면역학교실)
Jeong, Yong-Kee (동의대학교 미생물학과)
Lee, Won-Ho (부산대학교 생물학과)
Choi, Yung-Hyun (동의대학교 한의과대학 생화학교실)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.33, no.8, 2004 , pp. 1237-1245 More about this Journal
Abstract
Agaricus blazei Murill is a medicinal mushroom native to Brazil. It used to be a source of antitumor and immunoactive compounds and considered a health food in many countries. In the present study, it was examined the effects of water extract of A. blazei (WEAB) on the growth of human lung carcinoma cell line A549 in order to investigate the anti-proliferative mechanism by WEAB. Treatment of A549 cells to WEAB resulted in the growth inhibition, morphological change and induction of apoptotic cell death in a dose-dependent manner as measured by MTT assay and flow cytometric analysis. Flow cytometric analysis revealed that WEAB caused G2/M phase arrest of the cell cycle, which was associated with a down-regulation of cyclin A in both transcriptional and translational levels. WEAB treatment induced a marked up-regulation of cyclin-dependent kinase (Cdk) inhibitor p21, however, the levels of Cdk2, Cdc2, Wee1, Cdc25C and p53 expression were remained unchanged in WEAB treated cells. In addition, WEAB treatment inhibited the levels of cyclooxygenase (COX)-2 mRNA and protein without alteration of COX-l expression. Taken together, these findings suggest that WEAB may be a potential chemotherapeutic agent for the control of human lung carcinorma cells and further studies will be needed to identify the active compounds that confer the anti-cancer activity of WEAB. Once such compounds are identified, the mechanisms by which they exert their effects can begin to be characterized.
Keywords
Agaricus blazei Murill; G2/M arrest; apoptosis; p21;
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