Browse > Article
http://dx.doi.org/10.3746/jkfn.2004.33.7.1169

The Comparison of a Conformational Alteration of Ovalbumin Irradiated with Radiation of Gamma and Electron Beam  

변명우 (한국원자력연구소 방사선식품생명공학기술개발)
서지현 (한국원자력연구소 방사선식품생명공학기술개)
김재훈 (한국원자력연구소 방사선식품생명공학기술개)
김미리 (충남대학교 식품영양학)
오남순 (공주대학교 식품영양학)
이주운 (한국원자력연구소 방사선식품생명공학기술개발)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.33, no.7, 2004 , pp. 1169-1174 More about this Journal
Abstract
This study was carried out to assess the effects of electron beam (EB) radiation on the conformational changes of ovalbumin (OVA), based on the early works using gamma irradiation. The applied doses of OVA used were 3,5,7, and 10 kGy, respectively. The conformational alterations were measured with SDS-PAGE, GPC-HPLC, and competitive indirect enzyme-linked immunosorbent assay (Ci-ELISA) using monoclonal anti-OVA IgG antibody. Irradiation caused a degradation and/or an aggregation of OVA molecule. Immunochemical structures of irradiated OVA were altered by irradiation. Effects of gamma and electron beam radiation were similar at the same absorbed doses. These results may be used for inhibition of food allergy and development of immunogen with EB radiation.
Keywords
gamma radiation; electron beam radiation; ovalbumin; conformational changes;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Tuce Z, Janata ER, Milosavljevic BH. 2001. A kinetic study of the mechanism of radiation induced agglomeration of ovalbumin in aqueous solution. Radiat Phys Chem 62: 325-331.   DOI   ScienceOn
2 Lee JW, Park JH, Kim CJ, Shin HK. 1998. Monitoring thermally induced conformational changes in bovine muscle myosin solutions by competitive indirect enzyme-linked immunosorbent assay (Ci-ELISA). Int J Food Sci Technol 33: 411-418.   DOI   ScienceOn
3 Byun MW, Lee JW, Yook HS, Jo C, Kim HY. 2002. Application of gamma irradiation for inhibition of food allergy. Radiat Phys Chem 63: 369-370.   DOI   ScienceOn
4 Lee S, Lee S, Song KB. 2003. Effect of gamma-irradiation on the physicochemical properties of porcine and bovine blood plasma proteins. Food Chem 82: 521-526.   DOI   ScienceOn
5 Lee JI, Lee M. 1999. Effects of electron-beam irradiation and storage on cholesterol 7-derivatives products of meat. Korean J Food Sci Technol 31: 74-82.   과학기술학회마을
6 Kim WS, Chung MS, Ko YT. 1998. Effects of low dose gamma ray and electron beam irradiation on growth of microorganisms in beef during the refrigerated storage. Korean J Food Sci Ani Resour 18: 232-239.   과학기술학회마을
7 WHO/HPP/FOS. 1992. Review of the safety and nutritional adequacy of irradiated food. World Health Organization. Organisation Mondiale De La Sante. Provisional Edition. p 24-25.
8 Hayes DJ, Murano EA, Murano PS, Olson DG, Sapp SG. 1995. Food irradiation: a source book. Iowa State University Press, Ames. p 3-16.
9 Byun MW, Lee JW. 2003. Application of irradiation technology for food safety and security. Food Sci Ind 36: 25-41.
10 Byun MW, Kim JH, Lee JW, Park JW, Hong CS, Kang IJ. 2000. Effects of gamma radiation on the conformational and antigenic properties of a heat-stable major allergen in brown shrimp. J Food Prot 63: 940-944.   DOI
11 Koh KH, Whang K. 2002. Effect of electron beam irradiation on the oxidative and microbiological stability of ground pork during storage. Korean J Food Sci Ani Resour 22: 316-321.   과학기술학회마을
12 Lee JW, Kim JH, Sung CK, Kang KO, Shin MG, Byun MW. 2000. The changes of allergenic and antigenic properties of major allergen (Pen a 1) of brown shrimp (Penaeus aztecus) by gamma irradiation. Korean J Food Sci Technol 32: 822-827.   과학기술학회마을
13 Ahn HJ, Yook HS, Rhee MS, Lee CH, Cho YJ, Byun MW. 2002. Application gamma irradiation on breakdown of hazardous volatile N-nitrosamines. J Food Sci 67: 596-599.   DOI   ScienceOn
14 Hayashi T. 1991. Comparative effectiveness of gamma-rays and electron beams in food irradiation. In Food Irradiation. 2nd ed. Elsevier, London. p 169-206.
15 Lee JW, Yook HS, Cho KH, Lee SY, Byun MW. 2001. The changes of allergenic and antigenic properties of egg white albumin (Gal d 1) by gamma irradiation. J Korean Soc Food Sci Nutr 30: 500-504.   과학기술학회마을
16 Cho KH, Yook HS, Lee JW, Lee SY, Byun MW. 2001. Changes of binding ability of milk-hypersensitive patients' IgE to gamma-irradiated milk proteins. J Korean Soc Food Sci Nutr 30: 505-509.   과학기술학회마을
17 Jeon GR, Lee JW, Byun MW, Lee SY. 2002. Reduced allergenicities of irradiated egg white ovalbumin determined by skin prick test and ELISA inhibition test. J Asthma Allergy Clin Immunol 22: 711-719.
18 Moon S, Song KB. 2001. Effect of $\gamma$-irradiation on the molecular properties of ovalbumin and ovomucoid and protection by ascorbic acid. Food Chem 74: 479-483.   DOI   ScienceOn
19 Vieira FF, Del Mastro NL. 2002. Comparison of $\gamma$-radiation and electron beam irradiation effects on gelatin. Radiat Phys Chem 63: 331-332.   DOI   ScienceOn
20 Davies KJ, Delsignore ME. 1987. Protein damage and degradation by oxygen radicals: III. Modification of secondary and tertiary structure. J Biol Chem 262: 9908-9913.
21 Kume T, Matsuda T. 1995. Changes in structural and antigenic properties of proteins by radiation. Radiat Phys Chem 46: 225-231.   DOI   ScienceOn
22 Katial RK, Grier TJ, Hazelhurst DM, Hershey J, Engler RJM. 2002. Deleterious effects of electron beam radiation on allergen extracts. J Allergy Clin Immunol 110: 215-219.   DOI   ScienceOn
23 Kim KS, Yang JS, Kwon JH. 2003. Method of detection for irradiated foods. Korean J Food Preserv 10: 427-434.   과학기술학회마을
24 Lee JW, Kim JH, Yook HS, Kang GO, Lee SY, Hwang HJ, Byun MW. 2001. Effects of gamma radiation on the allergenic and antigenic properties of milk proteins. J Food Prot 64: 272-276.   DOI
25 Byun MW, Lee JW, Sep JH, Kim JH, Jo C, Kim DH, Chung HW. 2004. Changes of the immune reactivities of antibodies produced against gamma-irradiated antigen. Radiat Phys Chem in press.
26 Holm NW, Berry RJ. 1970. Manual on radiation dosimetry. Marcel Dekker Inc., New York.
27 Garrison WM. 1987. Reaction mechanisms in the radiolysis of peptides, polypeptides, and proteins. Chem Review 87: 381-398.   DOI
28 Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.   DOI   ScienceOn
29 Mathews CK, van Holde KE, Ahern KG. 1999. Biochemistry. 3rd ed. Addison Wesley Longman, San Francisco. p 133.
30 Davies KJ. 1987. Protein damage and degradation by oxygen radicals: I. General aspects. J Biol Chem 262: 9895-9901.