Browse > Article
http://dx.doi.org/10.3746/jkfn.2004.33.7.1112

DPPH Radical Scavenger Activity and Antioxidant Effects of Cham-Dang-Gui (Angelica gigas)  

강순아 (건국대학교 분자생명공학과 생명분자정보학센터)
한진아 (경희대학교 동서의학대학원 임상영양학전)
장기효 (삼척대학교 식품영양학)
조여원 (경희대학교 동서의학대학원 임상영양학전공)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.33, no.7, 2004 , pp. 1112-1118 More about this Journal
Abstract
This study was carried out to evaluate the free radical scavenging effect and antioxidant effect of Cham-Dang-Gui (Angelica gigas) on cyclophosphamide (CYP) injected rats. Rats were divided into five groups： CON (normal group), ANS (CYP-injected and normal diet group), AND (CYP-injected and normal diet and Cham-Dang-Gui-treated group), ALS (CYP-injected and low iron diet group), and ALD (CYP-injected and low iron diet and Cham-Dang-Gui-treated group). CYP (30 mg/kg) was intraperitoneally injected to rats for early 3 days. Saline or Cham-Dang-Gui was administrated orally for entire experimental period. DPPH radical scavenger activity was measured by DPPH method, it was shown higher in methanol extract (81.5％) than in water extract (66.3％) of Cham-Dang-Gui. We observed the preventive effects of Cham-Dang-Gui on lipid oxidation of liver and protein oxidation of plasma. Hepatic SOD and catalase activities were significantly higher in CYP-injected group (ANS) than CON group, but SOD activity was slightly lowered in Cham-Dang-Gui treated group than CYP-injected group (ANS). These results suggest that extract of Cham-Dang-Gui could be useful for functional materials to reduce the oxidation of lipids and protein induced by free radicals.
Keywords
Cham-Dang-Gui; antioxidant effect; DPPH radical scavenger activity; MDA;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Ghosh D, Das UB, Misro M. 2002. Protective role of alphatocopherol- succinate (provitamin E) in cyclophosphamideinduced testicular gametogenic and steroidogenic disorders: a correlative approach to oxidative stress. Free Radic Res 36: 1209-1218.   DOI   ScienceOn
2 Kim SJ, Song BG, Lee EJ, Kim HG, Kim JG. 2000. The effect of Angellica gigas on the relaxation of vascular smooth muscle and the expression of iNOS. Korean J Immunol 20: 60-67.
3 Kang SY, Lee KY, Park MJ, Kim YC, Markelonis GJ, Oh TH, Kim YC. 2003. Decursin from Angelica gigas mitigates amnesia induced by scopolamine in mice. Neurobiol Learn Mem 79: 11-18.   DOI   ScienceOn
4 Hur SK, Kim SS, Heo YH, Ahn SM, Lee BG, Lee SK. 2001. Effect of the grapevine shoot extract on free radical scavenging activity and inhibition of pro-inflammatory mediator production in raw 264.7 macrophages. J Applied Pharmacology 9: 188-193.
5 Roberforid M, Calderon PB. 1995. Free radicals and oxidation phenomena in biological system. Marcel Dekker, Inc, New York.
6 Chang NS, Ryu SM. 2001. Antioxidative effects of green tea powder diet against ethanol-induced oxidative damage in rat brain region. Kor J Nutr 34: 525-531.   과학기술학회마을
7 Rekha PS, Kuttan G, Kuttan R. 2001. Effect of Brahma Rasayana on antioxidant systems and cytokine levels in mice during cyclophosphamide administration. J Exp Clin Cancer Res 20: 219-223.
8 Cha BH, Oh SH, Yu CJ, Yang CY, Kim GY. 1992. Recombinant human granulocyte colony stimulation factor treatment of apriority agranulocytosis: 2 Case Report. Korean J Hematology 27: 325-329.
9 Ma JY, Ha CS, Sung HJ, Zee OP. 2000. Hemopoietic effects of Rhizoma Rehmanniae Preparata on Cyclophosphamideinduced pernicious anemia in rats. Kor J Pharmacogn 31: 325-334.
10 Kim DU, Jin JL, Lee JU, Han CH, Min SS, Kim HG, Park JW, Kim CC, Kim DI. 1992. Remedy of mixed immune in aplastic anemia patient. Korean J Hematology 27: 233-237.
11 Feusner J, Hastings C. 2002. Recombinant human erythropoietin in pediatric oncology: a review. Med Pediatr Oncol 39: 463-468.   DOI   ScienceOn
12 Valley AW. 2002. Overview of cancer-related anemia: focus on the potential role of Darbepoetin alfa. Pharmacotherapy 22 (9 Pt 2): 150S-159S.   DOI
13 Park KJ, Hong SB, Kim EH, Ma JY, Eun YA, Kim HS. 1998. Hemopoietic effects of deer blood on cyclophosphamide induced pernicious anemia. Kor J Pharmacogn 29: 283-292.   과학기술학회마을
14 Han DS. 1988. Herbology. Dong-Myoung publicating company, Seoul. p 201-202.
15 Han JA, Jang KH, Kang SA, Choue R. 2003. Hematological Effects of water extracts of Cham-Dang-Gui on cyclophosphamide induced anemic rat. Korean J Nutrition 36: 1013-1021.   과학기술학회마을
16 Tas F, Eralp Y, Basaran M, Sakar B, Alici S, Argon A, Bulutlar G, Camlica H, Aydiner A, Topuz E. 2002. Anemia in oncology practice: relation to diseases and their therapies. Am J Clin Oncol 25: 371-379.   DOI   ScienceOn
17 Das UB, Mallick M, Debnath JM, Ghosh D. 2002. Protective effect of ascorbic acid on cyclophosphamide-induced testicular gametogenic and androgenic disorders in male rats. Asian J Androl 4: 201-207.
18 Kaya H, Oral B, Ozguner F, Tahan V, Babar Y, Delibas N. 1999. The effect of melatonin application on lipid peroxidation during cyclophosphamide therapy in female rats. Zentralbl Gynakol 121: 499-502.
19 Claiborne A. 1984. Catalase activity. In CRC hanbook of methods for oxygen radical research. CRC Press, New York. p 283.
20 Harman D. 1987. The free radical theory of aging. In Mordern biological theories of aging. Warner HR, Butler RN, Sprott RL, Schneider EL, eds. Raven Press, NY. p 89.
21 Lee SI, Ahn DG, Shin MG, No SH, Lee YJ, Kim KH. 1982. Clinical Applied of Herb. Seung-Bo publishing company, Seoul. p 266-384.
22 Liu XL. 1984. Twelve cases of aplastic anemia treated mainly by ready Chinese drugs. Cung/ TSA Chih 25: 759-760.
23 Buege JA, Aust SD. 1978. Microsomal lipid peroxidation. Method Enzymol 52: 302-306.   DOI
24 Bradley RR, Cunniff PJ, Pereira BJ, Jaber BL. 1999. Hematopoietic effect of Radix angelicae sinensis in a hemodialysis patient. Am J Kidney Dis 34: 349-354.   DOI   ScienceOn
25 Colvin M. 1982. The alkylating agents. In Pharmacological principles of cancer treatment. Chabner B, ed. Saunders, Philadelphia. p 276.
26 Lim JP, Song YC, Kim JW, Ku CH, Eun JS, Leem KH, Kim DK. 2002. Free radical scavengers from the heartwood of Juniperus chinensis. Arch Pharm Res 25: 449-452.   DOI   ScienceOn
27 Shinnburber RO, Yu TC. 1958. Characterization of the red pigment formed in 2-thiobarbituric acid determination of oxidation rancibiliry. Food Res 23: 620-625.
28 Lee YK. 2000. Effects of dietary fatty acids and protein sources on lipid metabolism and DMBA induced mammary tumors in rats. PhD Dissertation. HanYang University.
29 Oliver CN, Ahn B, Moerman EJ, Goldstein S, Stadtman ER. 1987. Age-related changes in oxidized proteins. J Biol Chem 262: 5483-5492.
30 Marklund S. 1984. Pyrogallol antioxidant. In CRC hanbook of methods for oxygen radical research. CRC Press, New York. p 243.
31 Marklund S, Marklund G. 1974. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 468-471.
32 Kim HS, Heu IH, Lee SJ, Ahn HS. 1989. Immunologic characteristic on extract of old anter. Kor J Pharmacogn 38: 806-813.
33 Kim SM, Cho YS, Kim EJ, Bae MJ, Han JP, Lee SH, Sung SK. 1998. Effect of hot water extracts of Salvia miltiorrhiza Bge., Prunus persica Stokes, Angelica gigas Nakai and Pinus strobus on lipid oxidation. J Korean Soc Food Sci Nutr 27: 399-405.   과학기술학회마을
34 Choi Y, Kim M, Shin JJ, Park JM, Lee J. 2003. The antioxidant activities of the some commercial teas. J Korean Soc Food Sci Nutr 32: 723-727.   과학기술학회마을   DOI   ScienceOn
35 Kaya H, Oral B, Ozguner F, Tahan V, Babar Y, Delibas N. 1999. The effect of melatonin application on lipid peroxidation during cyclophosphamide therapy in female rats. Zentralbl Gynakol 121: 499-502.
36 Mortensen SR, Chanda SM, Hooper MJ, Padilla S. 1996. Maturational differences inchlorphyrifos-oxonase activity may contribute to age-related sensitivity to chlorpyrifos. J Biochem Toxicol 11: 279-287.   DOI   ScienceOn
37 Aebi HE. 1984. Catalase. In Methods in enzymatic analysis. 3rd ed. Verlag Chemie, Weinheim NY. Vol 3, p 273-285.
38 Kim SH, Lim JS. 1999. Effect of Korean traditional medicine on murine hematopoiesis (I) - Regulation of hematopoietic cytokine expression. Korean J Immunol 21: 165-174.
39 Medical college of Seoul University. 1993. Hematology. Seoul National Univ Publish., Seoul.