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http://dx.doi.org/10.3746/jkfn.2004.33.7.1092

Effect of the Crude Polysaccharide of Pleurotus eryngii on the Activation of Immune Cells  

강혜인 (순천대학교 식품영양학과)
김재용 (경북대학교 식품공학)
문광덕 (경북대학교 식품공학)
서권일 (순천대학교 식품영양학)
조영숙 (순천대학교 식품영양학)
이상대 (경남농업기술)
이성태 (순천대학교 생물학과)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.33, no.7, 2004 , pp. 1092-1097 More about this Journal
Abstract
The objective of the current study was to determine the effects of the crude polysaccharide isolated from fruit body of Pleurotus eryngii on mouse splenocytes, B cells, and macrophages in vitro. The crude polysaccharides directly induced the proliferation of spleen cells in a dose-dependent manner and increased IL-6 and IFN-${\gamma}$ synthesis. The crude polysaccharides also increased the proliferation of B cells in a dose-dependent manner. The production of immunoglobulin Gl, G2a and IgG3 in the presence of the crude polysaccharides was increased progressively in the culture supernatant. When the crude polysaccharide were used in macrophage cell line (RA W264.7) stimulation, there were marked induction of NO synthesis in a dose-dependent manner and IL-6, TNF- r and GM-CSF synthesis. These results suggest that the crude polysaccharide isolated from fruit body of Pleurotus eryngii seem to act as a potent immunomodulator causing augmentation of immune cell activity, and thus could be used as a biological response modifier having possible therapeutic effects against immunological disorders, without any side effects.
Keywords
Pleurotus eryngii; B cells; cytokines; nitric oxide; immunoglobulins;
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