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http://dx.doi.org/10.3746/jkfn.2004.33.7.1085

Effect of Bu-Zhong-Yi-Qi-Tang on Proliferation of T Cells  

채수연 (순천대학교 생물학과)
신성해 (순천대학교 생물학)
하미혜 (유용천연자원연구)
조성기 (원자력연구소 방사선식품생명공학)
김성호 (전남대 수의학)
변명우 (원자력연구소 방사선식품생명공학)
이성태 (순천대학교 생물학과)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.33, no.7, 2004 , pp. 1085-1091 More about this Journal
Abstract
Bu-Zhong-Yi-Qi- Tang extracts is a traditional oriental medicine in a mixture type exhibiting strong anti-bacterial, analgesic, and chemopreventive activities. In this study, we have evaluated effects of the total and polysaccharide fraction of Bu-Zhong-Yi-Qi- Tang extracts on the T cell proliferation, cytokine production, and induction of IL-2 receptor and MHC class n. For this experiment, we established CD4$^{＋}$ CD8$^{[-10]}$ T cell line producing IL-2 and IFN-${\gamma}$ when stimulated with ovalbumin antigen in the presence of antigen presenting cells. The significant effect of Bu-Zhong-Yi-Qi-Tang on antigen-induced T cell proliferation in the presence of antigen presenting cells was observed. The proliferation and IFN-${\gamma}$ production of T cells was increased in a dose dependent manner, and expression of IL-2 receptor on T cells and MHC class n molecule on antigen presenting cells was also induced in the presence of Bu-Zhong-Yi-Qi-Tang polysaccharide fraction. It was demonstrated that polysaccharide fraction of Bu-Zhong-Yi-Qi-Tang stimulates the antigen-induced T cell proliferation and the production of IFN-${\gamma}$ possibly through the increase of IL-2 receptor and MHC class n expression. Therefore Bu-Zhong-Yi-Qi-Tang can be regarded as a natural and useful immunomodulator having a relatively nonotoxic property. Further studies are needed to better characterize the nature of Bu-Zhong- Yi-Qi-Tang extract.
Keywords
Bu-Zhong-Yi-Qi-Tang; polysaccharide fraction; IL-2 receptor; IFN-${\gamma}$; MHC class II;
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