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http://dx.doi.org/10.3746/jkfn.2004.33.6.1017

Isolation of Trp, Thr Overproducing Strain of Saccharomyces cerevisiae  

염형준 (충남대학교 식품공학과)
이승현 (충남대학교 식품공학)
김선혜 (충남대학교 식품공학)
선남규 (충남대학교 식품공학)
안길환 (충남대학교 식품공학)
이봉덕 (충남대학교 축산학)
원미선 (한국생명공학연구)
송경빈 (충남대학교 식품공학과)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.33, no.6, 2004 , pp. 1017-1021 More about this Journal
Abstract
To isolate a mutant which overproduces threonine and tryptophan, mutants of Saccharomyces cerevisiae were screened after UV and EMS mutagenesis. Hydroxynorvaline, a Thr analogue was used for selection of a Thr-overproducing mutant after UV mutagenesis. Among 31 mutants, TC 5-1 was selected as the strain candidate, based on amino acid analysis. TC 5-1 was then treated by EMS mutagenesis for Trp overproduction. Eight mutants were selected using fluorotryptophan for Thr and Trp overproducing strains. Amino acid analysis results showed that TC 6-1 was the best strain since it had the highest amount of Thr and Trp among mutants.
Keywords
amino acid analogue; Saccharomyces cerevisiae; threonine; tryptophan;
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