Preparation of Ginseng Concentrate with High Content of Acidic Polysaccharide from White Tail Ginseng Marc

Preparation of ginseng concentrate with high content of acidic polysaccharide from white tail ginseng marc that was obtained after preparation of white tail ginseng extract. As a result of extraction of white tail ginseng under various concentrations of ethanol (0∼90%), both amount of acidic polysaccharide and extraction yield decreased by increasing the ethanol concentration. However, acidic polysaccharide extracted by water from white tail ginseng marc was increased in accordance with the increase of ethanol concentration. The optimal condition for the extraction of acidic polysaccharide from the marc was treatment of $\alpha$-amylase in 390∼650 unit/g residue/15 mL of distilled water for 5 min at 4$0^{\circ}C$. The amount of acidic polysaccharide in water extract of the marc was increased from 8.3% to 10.5% by the treatment of $\alpha$-amylase. A new ginseng extract mixture was manufactured by mixing 50% ethanol extract of white tail ginseng and water extract of alcoholic residue in the ratio of 8:2 (w/w). Crude saponin content and acidic polysaccharide content were 10.5% and 17%, respectively. The mixture had a same crude saponin content and twice acidic polysaccharide content comparing to 50% ethanol extract of white tail ginseng. It suggests that preparation of new ginseng concentrate with high content of acidic polysaccharide from white tail ginseng marc has high potencies in the utilization of waste material.

백삼 알코올 추출박을 이용한 산성다당체 다량 함유 백삼 농축액 제조

50% 에탄올 추출박으로부터 기존의 백삼농축액보다 산성다당체가 다량 함유된 백삼 농축액 제조 방법에 대해서 연구하였다. 50% 에탄올을 사용하여 제조한 백삼농축액의 추출 수율과 산성다당체 함량은 에탄올의 농도가 증가할수록 감소하였다. 반면에 백삼을 0∼90% 에탄올로 추출하고 남은 백삼박 내의 산성다당체 함량은 에탄올 농도가 증가할수록 증가하였다. 백삼박으로부터 산성다당체를 얻을 수 있는 최적의 조건은 $\alpha$-amylase를 390∼650 unit/g residue/15 mL 증류수의 농도로 4$0^{\circ}C$에서 5분 동안 처리하는 것으로 확인되었다. 50% 에탄을 추출 후 남은 백삼박에 $\alpha$-amylase를 처리함으로써 백삼박의 물추출액 중의 산성다당체 함량은 무처리구의 8.3%에서 10.5%로 증가하였다. 기존의 50% 에탄올 백삼추출농축액과 본 실험을 통하여 얻어진 백삼박 추출액을 8:2(w/w)로 혼합한 새로운 흔합백삼농축액을 제조한 결과 기존의 인삼농축액에 비해 조사포닌 함량은 비슷하며 약2배 정도의 산성다당체를 함유하고 색도가 짙은 농축액을 제조할 수 있었다.