Browse > Article
http://dx.doi.org/10.3746/jkfn.2004.33.3.487

Cytotoxicity and Chemosensitizing Effect of Camellia(Camellia japonica) Tea Extracts  

황은주 (동신대학교 생물자원산업화지원센터)
차영주 (동신대학교 산업용가속기 이용 생물연구센)
박민희 (동신대학교 산업용가속기 이용 생물연구센)
이장원 (동신대학교 산업용가속기 이용 생물연구센)
이숙영 (동신대학교 산업용가속기 이용 생물연구센터)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.33, no.3, 2004 , pp. 487-493 More about this Journal
Abstract
This study has been undertaken to increase availability of native camellia in Jeonnam as a medicinal resource and to isolate the effective components from them. Fresh leaf and flower of camellia, single camellia tea and camellia tea mixed with green tea, herbs were screened for cytotoxicity on MCF -7 (human breast adenocarcinoma pleual effusion), Calu-6 (human pulmonary carcinoma), SNU-601 (human gastric carcinoma) cells. Also their multidrug-resistance reversing activity were evaluated using drug sensitive AML-2/WT and multidrug-resistant AML-2/D100 cells. Among the camellia extracts, young leaf and camellia tea mixed with green tea had strong growth inhibitory effects in below 100 $\mu\textrm{g}$/mL against human cancer cells. In result, young leaf showed the strongest inhibitory effects on MCF -7 ($IC_{50}$/ = 100 $\mu\textrm{g}$/mL ↑), Calu-6 ($IC_{50}$/ = 79 $\mu\textrm{g}$/mL), and SNU -601 ($IC_{50}$/ = 39 $\mu\textrm{g}$/mL), and AML-2/WT ($IC_{50}$/ = 64 $\mu\textrm{g}$/mL). Chemosensitizing effect was the extracts of mature leaf ($IC_{50}$/ = 97 $\mu\textrm{g}$/mL, RF=3.0), roasted tea ($IC_{50}$/ = 76 $\mu\textrm{g}$/mL, RF = 2.6 ↑) and steam tea ($IC_{50}$/ = 70 $\mu\textrm{g}$/mL, RF=2.8 ↑) strongly potentiate vincristine cytotoxicity in AML-2/D100 cells. But their cytotoxicities to both sensitive AML-2/WT and resistant AML-2/D100 cells were in the same order of magnitude. This results indicate that crude extracts of camellia mature leaves would contain some principles which have chemosensitizing activity.
Keywords
Camellia japonica; cytotoxicity; chemosensitizing effect; multidrug-resistant (MDR);
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Kim JH, Lee SY, Cho SI. 2003. Anti-proliferative effect of Camellia japonica leaves on human leukemia cell line. Kor J Herbology 18: 93-98.   과학기술학회마을   DOI
2 Kim JH, Im WC, Park MH, Lee JH, Lee SY. 2003. Optimized conditions for making tea from camellia (Camellia japonica) leaf and flower and sensory evaluation. The Plant Resources Society of Korea. The 10th International Symposium. No. 16, Korean Supplement 2, p 34-35.   과학기술학회마을
3 Chang IL, Chi HJ. 1982. Toxicological evaluation of medicinal plants used for herbal drugs (III). Kor J Pharmacog 13: 55-61.   과학기술학회마을
4 Hwang EJ, Lee SY, Jeon WB, Pyo BS, Kim SM. 2003. Inhibitory effect of camellia (Camellia japonica) extract on growth of various cancer cells. 2003 Annual Meeting of The Korea Society of Medicinal Crop Science. Vol 11, Supplement No 2, p 220-221.
5 Cha YJ, Lee JW, Park MH, Hwang EJ, Lee SY. 2003. Major composition of leaf tea and flower tea using native camellia (Camellia japonica) in Korea, The Plant Resources Society of Korea. The 10th International Symposium. No.16, Korean Supplement 2, p 33.
6 송규택. 1978. 한국자원식물. 한국자원식물연구소. p 650.
7 Fujita Y, Fujita S, Yoshikawa H. 1973. Comparative biochemical and chemotaxonomical studies of the plants of Theaceae (I). Essential oils of Camellia sasanqua Thunb., C. japonica Linn., and Thea sinensis Linn. Osaka Kogyo Gijutsu Shidensho Kigo 25: 198-203.
8 Bhakuni DS, Goel AK, Jain S, Mehrotra BN, Patnaik GK. Prakash V. 1988. Screening of Indian plants for biological activity (Part III). J Exp Biol 26: 883-886.
9 Cha YJ, Park MH, Cho SI, Kim JH, Lee SY. 2002. The development of nutraceutical tea by native camellia in Korea. Annual Meeting and Seminar of the Korean Camellia Research Society.
10 송규택, 정현배, 봉희성. 1984. 한국자원식물, 미도문화사. p 650.
11 Kim HJ, Kim MK. 2003. Anticancer effect of persimmon leaf extracts on Korean gastric cancer cell. J Kor Soc F Nutr 36: 133-146.   과학기술학회마을
12 문교부. 1974. 한국동식물도감. 삼화출판사, 서울. 제15권, p 665.
13 Lee SY, Cha YJ, Lee JW, Hwang EJ, Kwon SJ, Cho SI. 2003. Physiological and pharmacological activities of nutraceutical tea by leaves and flowers of domestic camellia (Camellia japonica) The Plant Resources Society of Korea. The 10th International Symposium. No.16, Korean Supplement 2, p 48-49.
14 Itokawa H, Nakajima H, Ikuta A, Iitaka Y. 1981. Two teiterpenes from the flowers of Camellia japonica. Phytochemistry 20: 2539-2542.   DOI   ScienceOn
15 Ahmad N, Feyes DK, Nieminen AL, Agarwal R, Mukhtar H. 1997. Green tea constituent epigallocatechin-3-gallate and induction of apoptosis and cell cycle arrest in human carcinoma cells. J Natl Cancer Inst 89: 1881-1886.   과학기술학회마을   DOI   ScienceOn
16 Yoshikawa M, Harada E, Murakami T, Matsuda H, Yamahara J, Murakami N. 1994. Camelliasa ponins B1, B2, C1 and C2, new type inhibitors of ethanol absorption in rats from the seeds of Camellia japonica L. Chem Pharm Bull 42: 742-746.   DOI   ScienceOn
17 Kim SE, Hwang BY, Kim YH, Kim YC, Lee KS, Lee JJ. 1997. Multidrug-resistance reversing activity of medicinal plants. Kor J Pharmacogn 28: 174-178.   과학기술학회마을
18 이창복. 1982. 대한식물도감. 향문사, 서울. p 543.
19 Hwang EJ, Lee SY. 2003. Chemosensitizing activity against human leukemia cell of crude extracts of native camellia (Camellia japonica) in Jeonnam. 2003 Annual Meeting of the Korean Society of Applied Pharmacology. PB-11, p 85.   과학기술학회마을
20 Hwang EJ, Park MH, Pyo BS, Cha YJ, Lee SY. 2003. The cytotoxicity and chemosensitizing effects of native camellia (Camellia japonica) and nutraceutical camellia teas. 2003 Annual Meeting of The Plant Resources Society of Korea. No. 16, Korean Supplement 1, p 102.   과학기술학회마을
21 Tanaka H, Hirose M, Kawabe M, Sano M, Takesada Y, Hagisara A, Shirai T. 1997. Post-initiation inhibitory effects of green tea catechins on 7, 10-dimethylbenz[a]anthraceneinduced mammary gland carcinogenesis in female Sprague-Dawley rats. Cancer Lett 116: 47-52.   DOI   ScienceOn
22 Kim JR, Kim SY, Kim JH. 1995. Reversal effect of antihistamines on multidrug resistance in adriamycin- and vincristine-resistant L1210 variants. J Kor Cancer Assoc 27: 130-137.
23 Fischer SM, Leyton LJ, Lee ML, Locnislcar M, Belury MA, Maldve RE. 1992. Differential effects of dietary linoleic acid on mouse skin-tumor promotion and mammary carcinogenesis. Caner Res (Suppl) 52: 2049-2056.
24 최옥자, 김용두, 강성구. 1996. 동백종실 및 유박의 활용방안, 고유 농수산품목 세계화 대상품목의 연구조사, 동백나무편. 전라남도. p 331-425.