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http://dx.doi.org/10.3746/jkfn.2004.33.2.381

Determination of Pantothenic acid in Fortified Foods by HPLC  

최윤주 (식품의약품안전청 식품평가부)
장재희 (식품의약품안전청 식품평가부)
박혜경 (식품의약품안전청 식품평가부)
박건상 (식품의약품안전청 식품평가부)
구용의 (식품의약품안전청 식품평가부)
황인경 (서울대학교 식품영양학과)
김대병 (식품의약품안전청 식품평가부)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.33, no.2, 2004 , pp. 381-385 More about this Journal
Abstract
This study was conducted to develop an HPLC method for determining pantothenic acid in fortified foods which has typically been determined by microbiological assay (MBA) according to AOAC and Korean Food Code approved methods. Pantothenic acid was determined by reversed-phase ion-pair HPLC using UV absorption (200 nm) after extraction with 20 mM potassium phosphate solution by sonication. The recovery of spiked samples and detection limit (LOD) by HPLC were 83.5∼109.6% and 0.5 ppm (mg/kg), respectively. The LOD of the microbiological assay (MBA) was much lower than that of HPLC. The concentrations of pantothenic acid analyzed in all tested samples (n=13) confirmed compliance with declared label claims. The range of recovery ratio by the HPLC method when compared to the microbiological assay was 91.9∼117.6%. There was not significant difference (p<0.01) between the HPLC and MBA methods and the equation of the regression curve was y=1.1428x-0.2269 (r=0.9842). This proposed HPLC method for determining pantothenic acid appears to be suitable for determining pantothenic acid concentrations above 0.25 mg/100 g in fortified foods.
Keywords
pantothenic acid; HPLC method; microbiological assay;
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