Browse > Article
http://dx.doi.org/10.3746/jkfn.2004.33.2.271

Effect of Bu-Zhong-Yi-Qi-Tang on B Cell Development  

신성해 (순천대학교 생물학과)
채수연 (순천대학교 생물학과)
하미혜 (유용천연자원연구소)
조성기 (원자력연구소 방사선 식품생명공학팀)
김성호 (전남대학교 수의학과)
변명우 (원자력연구소 방사선 식품생명공학팀)
이성태 (순천대학교 생물학과)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.33, no.2, 2004 , pp. 271-277 More about this Journal
Abstract
This study was designed to evaluate the effect of Bu -Zhong-Yi-Qi-Tang extracts, a prescription of traditional oriental medicine, on development of the B cells. In the bone marrow cell cultures, progenitors viability, expressions of particular cell- surface proteins and production of immunoglobulins were investigated in the presence of Bu-Zhong-Yi-Qi-Tang extracts. The administration of Bu-Zhong -Yi-Qi-Tang polysaccharide fraction increased the viable cell numbers of the precursor B cells, and elevated expression levels of CD19/CD40 specific for pre-B cells after 10 days culture were demonstrated by flow cytometry analysis. The production of immunoglobulin M in the presence of polysaccharide fraction increased progressively in the culture supernatant, and preferentially induced class switching to IgG1, IgG2a and IgG3. These results indicated that Bu -Zhong -Yi-Qi -Tang strong1y correlated with the development of precursor B cells in the bone marrow cell culture. Therefore the polysaccharide fraction of Bu-Zhong-Yi -Qi-Tang might be a useful radioprotector, especially since it is a relatively non-toxic natural product. Further studies are needed to better characterize the protective nature of Bu-Zhong-Yi -Qi -Tang extract.
Keywords
Bu-Zhong-Yi-Qi-Tang; polysaccharide fraction; radioprotector; Pre-B cells; immunoglobulin class switching;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kim SH, Lee SE, Oh H, Kim SR. 2002. The radioprotective effects of Bu-Zhong-Yi-Qi-Tang. A prescription of traditional Chinese medicine. Amer J Chinese Med 30: 127-137.   DOI   ScienceOn
2 Kligerman MM, Shaw MT, Slavid M, Yudas JM. 1980. Phase I clinical studies with WR2721. Cancer Clin Trials 3: 217-221.
3 Zneg XL, Li XA, Zhang BY. 1992. Immunological and hematopoietic effect of Codonopsis pilosula on cancer patients during radiotherapy. Chung Kuo Chung Hsi I Chieh Ho Tsa Chih 12: 607-608.
4 Wang Y, Zhu B. 1996. The effect of angelica polysaccharide on proliferation and differentiation of hematopoietic progenitor cell. Chung Hua I Hsueh Tsa Chih 76: 363-366.
5 Hsu HY, Hau DM, Lin CC. 1993. Effects of kuei-pi-tang on cellular immunocompetence of gamma-irradiated mice. Am J Chin Med 21: 151-158.   DOI   ScienceOn
6 Hosokawa Y. 1986. Radioprotective effect of Chinese medicinal prescriptoins in mice. J Med Pharm Soc Wakan-Yaku 3: 164-169.
7 Mei QB, Tao TY, Cui B. 1991. Advances in the pharmacological studies of radix Angelica sinensis (Oliv) Diels (Chinese Danggui). Chin Med J Engl 104: 776-781.
8 Resenberg N, Kincade PW. 1994. B-lineage differentiation in normal and transformed-cells and the microenvironment that supports it. Curr Opin Immunol 6: 203-211.   DOI   ScienceOn
9 Li NQ. 1992. Clinical and experimental study on shen-qi injection with chemotherapy in the treatment of malignant tumor of digestive tract. Chung Kuo Chung Hsi I Chieh Ho Tsa Chih 12: 588-592.
10 이경화. 1974. 廣濟秘級. 계축문화사, 서울. p 47.
11 Yuan Y, Hou S, Lian T, Han Y. 1992. Studies of Rehmannia glutionosa Libosch. f. hueichingensis as a blood tonic. Chung Kuo Chung Yao Tsa Chih 17: 366-368.
12 Patt H, Tyee M, Straube RL. 1949. Cystein protects against x-irradiation. Science 110: 213-214.   DOI
13 Cairnie AB. 1983. Adverse effect of radioprotector WR2721. Radiat Res 94: 221-226.   DOI   ScienceOn
14 Miyanomae T, Frindel E. 1988. Radioprotection of hemopoiesis conferred by Acanthopanax senticosus Harms (Shigoka) administered before or after irradiation. Exp Hematol 16: 801-806.
15 Li XY, Takimoto H, Miura S, Yoshikai Y, Matsuzaki G, Nomoto K. 1992. Effect of the tradiational Chinese medicine, bu-zhong-yi-qi-tang (Japanese name: Hochu-ekki-to) on the protection against Listeria monocytogens infection in mice. Immunophamacol Immunotoxicol 14: 383-402.   DOI   ScienceOn
16 Ishikawa H, Manabe F, Zhongtao H, Yoshii S, Koiso K. 1992. The hormonal response to HCG stimulation on patients with male infertility before and after treatment with hochuekkito. Am J Chin Med 20: 157-165.   DOI   ScienceOn
17 黃甫中. 1992. 名醫指. 人民衛生出版社, 北京. p 88-90.
18 Kee BL, Paige CJ. 1995. Murine B-cell development-commitment and progression from multipotential progenitors mature B-lymphocytes. Intl Rev Cytol 157: 129-179.   DOI   ScienceOn
19 Kim SH, Cho CK, Yoo SY, Koh KH, Yun HG, Kim TH. 1993. In vivo radioprotective activity of Panax ginseng and diethyldithiocarbamate. IN VIVO 7: 467-470.
20 주진형. 1992. 丹溪心法附餘. 대성문화사, 서울. p 189-190.
21 Spangrude GJ. 1994. Biological and clinical aspects of hematopoietic stem cells. Annu Rev Med 45: 93-104.   DOI   ScienceOn
22 Ikeda S, Kaneko M, Kumazawa Y, Nishimura C. 1990. Protective activities of a Chinese medicine, Hochu-ekki-to, to impairment of hematopoietic organs and to microbial infection. Yakugaku Zasshi 110: 682-687.
23 Koshikawa N, Imal T, Takahashi I, Yamauchi M, Sawada S, Kansaku A. 1998. Effect of Hochu-ekki-to, Yoku-kansan and Saiko-ka-ryukotsuborei-to on behavioral despair and acetic acid-induced writhing in mice. Methods Find Exp Clin Pharmacol 20: 47-51.   DOI   ScienceOn
24 Stavnezer J. 1996. Immunoglobulin class switching. Curr Opin Immunol 8: 199-205.   DOI   ScienceOn
25 可得道. 1978. 中國醫學史略. 山西人民出版社, 山西城. p 92-97, 186.
26 Lu G, Yang M, Shen Y, Meng J. 1991. The absorption of Fe, Zn, Cu in siwu, sijunzi, and Liuwei dihuang decoction by small intestine in rats. Chung Kuo Chung Yao Tsa Chih 16: 297-298.
27 Lee SE, Oh H, Yang JA, Jo SK, Gyun MW, Yee ST, Kim SH. 1996. Radioprotective effects of two traditional Chinese medicine prescriptions. Si-Wu-Tang and Si-Jun-Zi-Tang. Am J Chin Med 27: 387-396.
28 Hsu HY, Ho YH, Lian SL, Lin CC. 1993. Preliminary study on the anti-radiation effect of jen-sheng-yang-yang-tang. Am J Chin Med 21: 187-195.   DOI   ScienceOn
29 Hsu HY, Ho YH, Lian SL, Lin CC. 1991. Preliminary study on antiradiation effect of kuei-pi-tang. Am J Chin Med 19: 275-284.   DOI   ScienceOn
30 Ito H, Shimura K. 1985. Studies on the antitumor activity of traditional Chinese medicine. Gan To Kagaku Ryoho 12: 2145-2148.
31 Murakami Y. 1998. Clinical effect of hotyuekkito (buzhongyiqitang) on symptoms due to renal ptosis and stress incontinence. Hinyokika Kiyo 34: 1841-1843.
32 Milas L, Murray D, Brock WA, Meyn RE. 1988. Radioprotectors in tumor radiotherapy: Factors and settings determining therapeutic ratio. Pharmacol Ther 39: 179-189.   DOI   ScienceOn
33 Hsu HY, Lian SL, Lin CC. 1990. Radioprotective effect of Ganoderma iucidum (Leyss. ex. Fr.) Karst after X-ray irradiation in mice. Am J Chin Med 18: 61-69.   DOI   ScienceOn
34 Funk PE, Kincade PW, Witte PL. 1994. Native associations of early hematopoietic stem-cells and stromal cells isolated in bone-marrow cell aggregates. Blood 83: 361-369.
35 Milas L, Hunter M, Reid BO, Thames Jr HD. 1982. Protective effects of S-2-(3-aminopropylamino) ethylphosphorothioic acid against radiation damage of normal tissues and a fibrosarcoma in mice. Cancer Res 42: 1888-1987.
36 Ohta S, Sakurai N, Sato Y, Inoue T, Shinoda M. 1990. Studies on chemical protectors against radiation. XXX. Radioprotective substances of cnidii rhizoma. Yakugaku Zassh 110: 746-754.
37 Washburn LC, Carlton JE, Hayes RL. 1974. Distribution of WR-2721 in normal and malignant tissue of mice and rats bearing solid tumors: dependence on tumor type, drug does and species. Radiat Res 59: 483-575.
38 Wang HB, Zheng QY, Ju DW, Fang J. 1993. Effects of Phytolacca acinosa polysaccharides II on lymphocyte proliferation and colony stimulating factor production from mice splenocytes in vitro. Yao Hsueh Hsueh Pao 28: 490-493.