Browse > Article
http://dx.doi.org/10.3746/jkfn.2003.32.7.1082

Effects of Pine Needle Butanol Fraction on Membrane Fluidity and Oxidative Stress in Liver Membranes of Rats  

최진호 (부경대학교 생화학교실)
김대의 (부경대학교 생화학교실)
최민경 (최민경요리아카데미)
조원기 (조아제약㈜)
김창목 (한국산업정보기술연구원 생화학실)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.32, no.7, 2003 , pp. 1082-1087 More about this Journal
Abstract
This study was designed to investigate the effects of butanol (BuOH) fraction of pine (Pinus densiflora Sieb et Zucc) needle extract on membrane fluidity (MF), basal and induced oxygen radicals (BOR and IOR), lipid peroxide (LPO) and oxidized protein (OP) as an oxidative stress, and lipofuscin (LF) in liver membranes of Sprague-Dawley male rats. The rats were fed basic diets (control group) and experimental diets (BuOH-25, BuOH-50 and BuOH-100) prepared with 25, 50 and 100 mg added to basic diet for 45 days. MFs were significantly increased (about 16∼22%) in mitochondria of BuOH-50 and BuOH-100 groups compared with control group (p<0.01∼0.001) BOR and IOR formations in mitochondria were significantly decreased (11∼17% and 11∼28%, respectively) in these three BuOH groups (p<0.05∼0.001), while BOR and IOR formations in microsomes were significantly decreased (11∼24%) in BuOH-50 and BuOH-100 groups, and (15∼24%) in these three BuOH groups compared with control group (p<0.05∼0.001; p<0.01-0.001). LPO levels were significantly decreased (9% and 9∼13%, respectively) in mitochondria of BuOH-100 and microsomes of BuOH-50 and BuOH-100 groups (p<0.05∼0.01), whereas OP levels were significantly decreased (10∼12%) in mitochondria of BuOH-50 and BuOH-100 groups compared with control group (p<0.05). LF formations were significantly decreased (8∼9%) in BuOH-50 and BuOH-100 groups (p<0.05). These results suggest that butanol fraction of pine needle extract may playa effective role in an attenuating an oxidative stress and increasing a membrane fluidity.
Keywords
pine (Pinus densiflora Sieb et Zucc); lipid peroxide (LPO); oxidized protein (OP); carbonyl group; membrane fluidity (MF);
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Fletcher BL, Dillard CJ, Tappel SAL. 1973. Measurement of fluorescent lipid peroxidation products in biological systems and tissues. Anal Biochem 52: 1-9.   DOI   ScienceOn
2 Yagi H, Katoh S, Akiguchi I, Takeda T. 1988. Age-related deterioration of ability of acquisition in memory and learning in SAMP-8 as an animal model of disturbances in recent memory. Brain Research 474: 86-93.   DOI   ScienceOn
3 Choi JH, Kim DW, Kim JH, Kim KS, Lee JS. 1997. Effect of pine needle extract (PNE) on physiological activity of SD rats I. Feeding effect of PNE on lipid and oxygen radical metabolisms in serum of SD rats. Korean J Life Sci 7:371-376.   과학기술학회마을
4 Yu BP. 1996a. Aging and oxidative stress: Modulation by dietary restriction. Free Rad Biol Med 21: 651-668.   DOI   ScienceOn
5 Kong Z, Liu Z, Ding B. 1995. Study on the antimutagenic effect of pine needle extract. Mutat Res Aug, 347: 101-104.   DOI   ScienceOn
6 Namba T. 1980. Colored Illustrations of WAKAN-YAKU. Hoikusha Publishing Co., LTD, Osaka. Vol II, p 82-83.
7 Choi JH, Yu BP. 1990. Unsuitability of TBA test as a lipid peroxidation marker due to prostaglandin synthesis in the aging kidney. Age 13: 61-64.   DOI
8 Lowry OH, Roseborough NJ, Farr LA, Randall RJ. 1951. Protein measurement with the Folin-Phenol reagent. J Biol Chem 193: 265-275.
9 Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn B, Shaltiel S, Stadtman ER. 1990. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol 186: 464-478.   DOI
10 Steel RGD, Torrie JH. 1960. Principles and procedures of stastistics. McGrawhill, New York.
11 Kang YH, Park YK, Ha TY, Moon KD. 1996b. Effects of pine needle extracts on enzyme activities of serum and liver, and liver morphology in rats fed high fat diet. J Korean Soc Food Nutr 25: 374-378.   과학기술학회마을
12 Heron DS, Shinitzky M, Hershkowitz M, Samuel D. 1980. Lipid fluidity markedly modulates the binding of serotonin to mouse brain membranes. Proc Natl Acad Sci 12: 7463-7467.
13 Choi JH, Kim DI, Park SH, Baek SJ, Cho WK, Lee JH, Kim HS. 2002. Effects of butanol fraction of pine needle (Pinus densiflora) on oxygen radicals and their scavenger enzymes in liver of SD rats. Korean J Nutr 35: 291-295.
14 Kim EJ, Jung SW, Choi KP, Ham SS, Kang HY. 1998. Cytotoxic effect of the pine needle extract. Korean J Food Sci Technol 30: 213-217.   과학기술학회마을
15 Yu BP, Lee DW, Marler CG, Choi JH. 1990. Mechanism of food restriction: Protection of cellular homeostasis. Soc Exp Biol Med 193: 13-15.   DOI
16 Choi JH, Kim JH, Kim DW, Kim KS, Lee JS, Baek YH. 1998a. Effect of pine needle extract (PNE) on physiological activity of SD rats II. Feeding effect of PNE on oxygen radicals and their scavenger enzymes in brain membranes of SD rats. Korean J Life Sci 8: 91-96.   과학기술학회마을
17 Kim JD, Yoon TH, Choi M, Im KJ, Ju JS, Lee SY. 1990. Effect of dietary supplementation with pine leaf on lipid parameters in rats. Kor J Gerontol 1: 47-50.
18 Kang YH, Park YK, Ha TY, Moon KD. 1996a. Effects of pine needle extracts on serum and liver lipid contents in rats fed high fat diet. J Korean Soc Food Nutr 25: 367-373.   과학기술학회마을
19 Choi JH, Kim DI, Park SH, Kim DW, Lee JS, Kim HS. 1999. Effects of pine needle (Pinus densiflora) extract on membrane fluidity and oxidative stress in liver membranes of rats. Korean J Life Science 9: 473-480.   과학기술학회마을
20 Lebel CP, Odunze IN, Bondy A Jr, Bondy SC. 1989. Perturbations in cerebral oxygen radical formation and membrane order following vitamin E deficiency. Biochem Biophysic Res Com 163: 860-866.   DOI   ScienceOn
21 Choi JH, Yu BP. 1995. Brain synaptosomal aging: Free radicals and membrane fluidity. Free Rad Biol & Med 18: 133-139.   DOI   ScienceOn
22 Choi JH, Kim JH, Kim DW, Hwang CH, Kim DI, Lee JS. 1998b. Effect of pine needle extract (PNE) on physiological activity of SD rats III. Feeding effect of PNE on fluidity and neurotransmitter-related enzymes in brain membranes of SD rats. Korean J Life Sci 8: 167-172.   과학기술학회마을