Browse > Article
http://dx.doi.org/10.3746/jkfn.2003.32.4.586

Pharmacological Effect of Hawangyeonhaedoktang on Experimental Triglyceride Accumulated HepG2 Cells  

차재영 (동아대학교 식품과학부)
김대진 (동아대학교 식품과학부)
김석환 (동아대학교 식품과학부)
김영길 (동아대학교 식품과학부)
조영수 (동아대학교 응용생명공학부)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.32, no.4, 2003 , pp. 586-590 More about this Journal
Abstract
The pharmacological effect of Korean-Chinese traditional herbal medicine, Hawangyeonhaedoktang (HT) on experimentally induced-triglyceride accumulation in cultured human hepatocyte HepG2 cells was studied. HePG2 cells were cultured in the Dulbecco's modified Eagle's (DME) medium without (Control medium) or with HT (0.5 mg/mL and 5.0 mg/mL) containing 1 mM oleate, 0.2% bovine serum albumin (BSA), and glucose 4.5 mg/mL for 6 and 24 hours in experiment I and 2 mM oleate, 0.5% BSA, and glucose 4.5 mg/mL for 6, 24 and in hours in Experiment II or 1 and 3 hours in Experiment III. Oleate ［$^{14}$ C］(0.5 $\mu$Ci/mL medium) added as a radioactive lipid precursor in the experiment I. In the experiment I, the intracellular triglyceride concentration was decreased remarkably during incubation for 6 and 24 hours, in a dose-dependent manner. At the same time, HT caused a decrease in the incorporation of ［$^{14}$ C］ oleate into intracellular triglyceride fraction and the secretion of triglyceride labeled with ［$^{14}$ C］ oleate into medium. In the experiment II and III compared to experiment I, the triglyceride accumulation in HepG2 cells was occurred, and HT prevented the accumulation of triglyceride during incubation for 24 and 48 hours. This result suggest that HT prevent the triglyceride accumulation in human hepatocytes by its inhibiting action on the intercellular triglyceride biosynthesis.
Keywords
Korean-Chinese herbal medicines; hawnagyeonhaedoktang; HepG2 cells; triglyceride;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Saku K, Hirata K, Zhang B, Liu R, Ying H, Okura Y, Yoshinaga K, Arakawa K. 1992. Effects of Chinese herbal drugs on serum lipids, lipoproteins and apolipoproteins in mild to moderate essential hypertensive patients. J Hum Hypertens 6: 393-395.
2 Yanagita T, Hara E, Yotsumoto H, Rahman SM, Han SY, Cha JY, Yamamoto K. 1999. NK-104, a potent new 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, enhances posttranslational catabolism of apolipoprotein B-100 and inhibits secretion of apolipoprotein B-100 and triacylglycerols from HepG2 cells. Current Therapeutic Res 60: 423-434.   DOI   ScienceOn
3 Cha JY, Cho YS, Yanagita T. 1999. Effects of linoleic acid and serum albumin concentrations on lipid metabolism in HepG2 cells. J Korean Agric Chem Biotechnol 42: 229-234.
4 Lowry OH, Rosenbrough NJ, Farr AL, Randall RT. 1951. Protein measurement with the folin phenol reagent. J Biol Chem 193: 265-275.
5 Ohta Y, Sasaki E, Nishida K, Kongo M, Hayashi T, Nagata M, Ishiguro I. 1998. Inhibitory effect of Oren-gedoku-to (Huanglian-Jie-Du-Tang) extract on hepatic triglyceride accumulation with the progression of carbon tetrachlorideinduced acute liver injury in rats. J Ethnopharmacol 61: 75-80.   DOI   ScienceOn
6 Sorenson TIA, Orholm M, Bentsen KD, Hoybye G, Eghoje K, Christoffersen P. 1984. Prospective evaluation of alcohol abuse and alcoholic liver in men as predictors of development of cirrhosis. Lancet 2: 241-244.   DOI   ScienceOn
7 Wildi SM, Reich J, Flury R, Lauper U, Risti B, Mullhaupt B, Meyenberger C. 2002. Acute fatty liver in pregnancy: clinical and histopathological course. Case report. Schweiz Rundsch Med Prax 91: 267-73.
8 Teramoto T, Kato T, Kinoshita M. 1986. Antilipidemic action of Dai-saiko-to on experimental hyperlipidemia rats. The Clinical Report (Kiso To Rinsho) 20: 105-109.
9 Cha JY, Mameda Y, Oogami K, Yamamoto K, Yanagita T. 1998. Association between hepatic triacylglycerol accumulation induced by administering orotic acid and enhanced phophatidate phosphohydrolase activity in rat. Biosci Biotechnol Biochem 62: 508-513.   DOI   ScienceOn
10 Hwang JM, Wang CJ, Chou FP, Tseng TH, Hsieh YS, Lin WL, Chu CY. 2002. Inhibitory effect of berberine on tertbutyl hydroperoxide-induced oxidative damage in rat liver. Arch Toxicol 76: 664-670.   DOI   ScienceOn
11 Bligh EG, Dyer WJ. 1959. A rapid method of extraction and purification. Can J Biochem Physiol 37: 911-917.   DOI
12 Dashti N. 1992. The effect of low density lipoproteins, cholesterol and 25-hydroxycholesterol on apolipoprotein B gene expression in HepG2 cells. J Biol Chem 267: 7160-7169.
13 Duncan DB. 1957. Mutiple range test for correlated and heteroscedastic means. Biometrics 13: 164-176.   DOI   ScienceOn
14 Yamamoto K, Ogawa Y, Yanagita T, Morito F, Fukushima N, Ozaki I, Mizuta T, Setoguchi Y, Sakai T. 1995. Phamacological effects of Dai-saiko-to on lipid biosynthesis in cultured human hepatocyte HepG2 cells. J Ethnopharmacol 46: 49-54.   DOI   ScienceOn
15 Okuda H. 1986. Effect of Dai-saiko-to and Saiko-karyuktaubosei-to on hyperoxidation lipid rats and intestinal absorption of lipid. Kampo-Igaku 10: 16-21.
16 Yano H, Mizoguchi A, Fukuda K, Haramaki M, Ogasawara S, Monosaki S, Kojiro M. 1994. The herbal medicine Shosaiko-to inhibits proliferation of cancer cell lines by inducing apotosis and arrest at the Go/G1 phase. Cancer Reseach 54: 448-454.
17 Yotsumoto H, Yanagita T, Yamamoto K, Ogawa Y, Cha JY, Mori Y. 1997. Inhibitory effects of Oren-Gedoku-to and its components on cholesteryl ester synthesis in cultured human hepatocyte HepG2 cells: Evidence from the cultured HepG2 cells and in vitro assay ACAT. Planta Med 63: 141-145.   DOI   ScienceOn
18 Umeda M, Amagaya S, Ogihara Y. 1989. Effect of shosaikoto, daisaikoto and sannoshashinto (traditional Japanese and Chinese medicines) on experimental hyperlipidemia in rats. J Ethnopharmacol 26: 255-269.   DOI   ScienceOn