Browse > Article
http://dx.doi.org/10.3746/jkfn.2002.31.6.1065

Effects of YK-209 Mulberry Leaves on Antioxidative Defense System of Liver in Streptozotocin-Induced Diabetic Rats  

유수경 (대구카톨릭대학교 식품영양학과)
이순재 (대구카톨릭대학교 식품영양학과)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.31, no.6, 2002 , pp. 1065-1070 More about this Journal
Abstract
The purpose of this study was investigated the effects of YK-209 mulberry leaves on antioxidative defense system of liver in diabetic rats induced with streptozotocin (STZ). Male Sprague-Dawley rats weighing 100$\pm$10 g were randomly assigned to one normal and four STZ-induced diabetic groups; YK-209 mulberry leaves free diet (DM group),0.1% YK-209 mulberry leaves diet (DM-0.1Y group),0.2% YK-209 mulberry leaves diet (DM-0.2Y group) and 0.4% YK-209 mulberry leaves diet (DM-0.4Y group). Diabetes was induced by intravenous Injection of 55 mg/kg body weight of STZ in sodium citrate buffer (pH 4.3) via tail vein after 4 weeks feeding of experimental diets. Rats were sacrificed at the 9th day of diabetic states. Liver weight in all four diabetic groups were higher than normal group, but YK-209 mulberry supplementation groups were lower than DM group. Hepatic superoxide dismutase (SOD) activity was significantly decreased in all diabetic groups, compared with normal group. Hepatic glutathione peroxidase (GSHpx) activity was 7.3% decreased in DM group, compared with normal group, but those of DM-0.1Y and DM-0.2Y groups were maintained the normal level. The hepatic thiobarbituric acid reactive substances was markedly increased by 144% in DM group, compared with normal group, but those of DM-0. 1Y, DM-0.2Y groups were maintained the normal level. The contents of lipofuscin in liver were increased by 100% in DM group compared with normal group, but those of DM-0. 1Y, DM-0.2Y and DM-0.4Y groups were decreased to 42% 43% and 44%, respectively, compared with DM group. The hepatic superoxide radical (0$^2$-) contents in DM group were increased to 81%, compared with normal group, but those of DM-0.1Y and DM-0.4Y groups were similar to those of normal group. The present result indicate that YK-209 mulberry leaves regarded to suppress lipid peroxidation as an free radical scavenger system by the inhibition of oxidative stress.
Keywords
YK-209 mulberry leaves; diabetes antioxidative system;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Azzi A, Montecucco C, Richter C. 1975. The use of acetylated ferricytochrome c for the detection of superoxide radicals produced in biological membranes. Biochem Biophys Res Commun 65: 597-603.   DOI   ScienceOn
2 Lawrence RA, Burk RF. 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 71: 952-958.   DOI   ScienceOn
3 Fletcher BL, Dillard CJ, Tappel AL. 1973. Measurement of fluorescent lipid peroxidation products in biological systems and tissues. Anal Biochem 52: 1-9.   DOI   ScienceOn
4 Kim SY, Lee WC, Kim HB, Kim SK. 1998. Antihyperlipidemic effects of methanol extracts from mulberry leaves in cholesterol induced hyperlipidemia in rats. J Kor Soc Food Sci Nutr 27: 1217-1222.   과학기술학회마을
5 Satoh K. 1978. Serum lipid peroxide in cerebrovascular disorders determined by a new colormetric method. Clin Chim Acta 90: 37-43.   DOI   ScienceOn
6 Lalla E, Lamster IB, Drury S, Fu C, Schmidt AM. 2000. Hyperglycemia, glycoxidation and receptor for advanced glycation endproducts: potential mechanisms underlying diabetic complications, including diabetes-associated periodontitis. Periodontol 23: 50-62.   DOI   ScienceOn
7 West IC. 2000. Radicals and oxidative stress in diabetes. Diabet Med 17: 171-180.   DOI   ScienceOn
8 Baynes JW. 1991. Role of oxidative stress in development of complications in diabetes. Diabetes 40: 405-412.   DOI   ScienceOn
9 Jain SK, McVie R, Jaramillo JJ, Palme M, Smith T, Meachum ZD, Little RL. 1996. The effect of modest vitamin E supplementation on lipid peroxidation products and other cardiovascular risk factors in diabetic patients. Lipids 31: S87-S90.   DOI
10 Sato Y, Hotta N, Sakamoto N, Matsuoka S, Ohishi N, Yagi K. 1979. Lipid peroxide level in plasma of diabetic patients. Biochem Med 21: 104-107.   DOI   ScienceOn
11 Griesmacher A, Kindhauser M, Andert SE, Schreiner W, Toma C, Knoebl P, Pietschmann P, Prager R, Schnack C, Schernthaner G. 1995. Enhanced serum levels of thiobarbituricacid-reactive substances in diabetes mellitus. Am J Med 98: 469-475.   DOI   ScienceOn
12 Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the antioxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474.   DOI   ScienceOn
13 Naitoh K. 1968. Studies on the micro constituent in mulberry leaves part 2. Isolation of rutin and quercetin from mulberry leaves. Nippon Nogei Kagaku Kaishi 42: 422-425.
14 Bus JS, Aust SD, Gibson JE. 1975. Lipid peroxidation: a possible mechanism for paraquat toxicity. Res Commun Chem Pathol Pharmacol 11: 31-38.
15 Onogi A, Osawa K, Yasuda H, Sakai A, Morita H, Tokawa H. 1993. Flavonol glycosides from the leaves of Morus alba. Shoyakugaku Zasshi 47: 423-425.
16 Haper MJK, Catherine J, Norris WE. 1981. Friedrichs and A Moreno Poiy I Caccelerates ovum transport in the rabbit by a prostaglandin-mediated mechanism. J Reprod Fert 63: 81-89.   DOI   ScienceOn
17 Kwag OG, Yang JA, Rhee SJ. 1999. Effects of vitamin E on the antioxidative defense system of kidney in streptozotocin -induced diabetic rats. J Korean Soc Food Sci Nutr 28: 654-662.   과학기술학회마을
18 Chai YM, Park MR, Rhee SJ. 1998. Effects of green tea catechins on the antioxidative defense system and lipofuscin levels heart in streptozotocin-induced diabetic rats. Kor J Gerontol 8: 96-103.
19 Maytin M, Leopold J, Leopold J, Lascalzo J. 1999. Oxidant stress in the vasculature. Curr Atheroscler Rep 1: 156-164.   DOI   ScienceOn
20 Chance B, Sies H, Boveris A. 1979. Hydroperoxide metabolism in mammalian organs. Physiol Rev 59: 527-605.   DOI
21 Kim JS, Kang SS, Lee MW, Kim OK. 1995. Isolation offlavonoids from the leaves of Aralia continentails. Kor J Phamacogn 26: 239-243.
22 Awasthi YC, Beutler E, Srivastava SK. 1975. Purification and properties of human erythrocyte glutathione peroxidase. J Biol Chem 250: 5144-5149.
23 Bompart GJ, Prevot DS, Bascands JL. 1990. Rapid automated analysis ofglutathion reductase, peroxidase and S-transferase activity: application to cisplatin-induced toxicity. Clin Biochem 23: 501-504.   DOI   ScienceOn
24 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. J Biol Chem 193: 265-275.
25 Kim SY, GA JJ, Lee WC, Ryu KS, Lee KR, Kim YC. 1999. Antioxidative flavonoids from the leaves of Morus alba. Arch Pharm Res 22: 81-85.   과학기술학회마을   DOI   ScienceOn
26 Kang SS, Woo WS. 1984. Flavonol glycosides from the leaves of Zizyphus jujuba. Kor J Pharmacogn 15: 170-178.   과학기술학회마을
27 Obrosova IG, Fathallah L, Green DA. 2000. Early changes in lipid peroxidation and antioxdative defense in diabetic rat retina: effect of DL-alpha-lipoic acid. Eur J Pharmacol 398: 139-146.   DOI   ScienceOn
28 Robak J, Gryglewski RI. 1988. Flavonoids are scavengers of superoxide anions. Biochem Pharmacol 37: 837-841.   DOI   ScienceOn
29 Wolff SP, Jiang ZY, Hunt JV. 1991. Protein glycation and oxidative stress in diabetes mellitus and aging. Free Radic Biol Med 10: 339-352.   DOI   ScienceOn
30 Shin KH, Young HS, Lee TW, Choi JS. 1995. Studies on the chemical component and antioxidative effects of solanum lyratum. Kor J Pharmacogn 26: 130-138.