Browse > Article
http://dx.doi.org/10.3746/jkfn.2002.31.5.826

Effects of YK-209 Mulberry Leaves on HMG-CoA Reductase and Lipid Composition of Liver in Streptozotocin-Induced Diabetic Rats  

홍정희 (대구가톨릭대학교 식품영양학과)
이순재 (대구가톨릭대학교 식품영양학과)
박모라 (상주대학교 식품영양학과)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.31, no.5, 2002 , pp. 826-833 More about this Journal
Abstract
This study was conducted to investigate the effects of YK-209 mulberry leaves on HMG-CoA reductase activity and lipid composition of liver in streptozotocin-induced diabetic rats. Sprague-Dawley male rats weighing 100 $\pm$ 10 B were randomly assigned as a normal group and four STZ-induced diabetic groups according to the level of dietary mulberry leaves supplement. The experimental diets were fed ad libidum, so that diabetes was experimentally induced by intravenous injection of STZ 55 mg/kg of body weight after feeding for 3 weeks. Animals were sacrificed on the 9th day of diabetic states. The levels of serum triglyceride, total-cholesterol and LDL-cholesterol in DM group were higher than mulberry leaves supplemented groups and normal group, but those of the mulberry leaves supplemented groups were significantly decreased to normal level. In contrast, the leavels of serum HDL-cholesterol in DM group was significantly reduced than that of normal group, but mulberry leaves supplemented groups were increased to normal level. Atherogenic index in DM group was higher about 3 fold than the normal group but the DM-0.1Y and DM-0.2Y groups were maintained the normal level. Contents of total lipid and triglyceride of liver in DM group were significantly lower than that of normal group, but the mulberry Leaves supplemented groups increased than that of DM group. The contents of hepatic cholesterol in DM group was 160% higher than that of normal group, but the mulberry leaves supplementation groups maintained the normal level. The activity of hepatic 3-hydroxy -3-methylglutaryl Coenzyme A (HMG-CoA) reductase in DM group was 43% lower than that of normal group, but had no significant difference between DM-0.1Y, DM-0.2Y and normal groups. In conclusion, YK-209 mulberry loaves has improving effect of the lipid metabolism in STZ-induced diabetic rats through hepatic HMG-CoA reductase activity, and the change of lipids contents in serum and liver.
Keywords
YK-209 mulberry leaves; diabetes mellitus; cholesterol; HMG-CoA reductase;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Yen GC, Wu SC, Duh PD. 1996. Extraction and identification of anti-oxidant components from the leaves of mulberry (Morus alba L.). J Biol Chem 261: 12879-12882.
2 Sheng J, Yuji M, Kenji M, Shinji K, Tadashi N, Katsuto T, Masharu K, Seiichiro T. 1988. Increased activity of intestinal Acyl-Co A : cholesterol acyltransferase in rats with streptozotocin- induced diabetes and restoration by insulin supplement. Diabetes 37: 342-346.   DOI   ScienceOn
3 Pain VM, Garlick P. 1974. Effect of streptozotocin diabetes and insulin treatment on the rate of protein synthesis in tissues of the rat in vivo. J Biol Chem 249: 4510-4
4 Courtiere A, Molard F, Reyband J. 1995. Differential effects of in vitro peroxidation on peripheral- and central-type benzodiazepine receptors. Protection by diverse antioxidants. Biochem Pharmacol 50: 1815-1822.   DOI   ScienceOn
5 Hulcher FH, Oleson WH. 1973. Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl CoA reductase by measurement of coenzyme A. J Lipid Res 14: 625-631.
6 Ganda OP. 1985. Pathogenesis of macrovascular disease including in the influence of lipid. In Joslins' Diabetes Mellitus. 12th ed. Lea and Febiger, Philadelphia. p 217.
7 Li SZ. 1978. Compendium of materia medica. People's Medical Publishing House, Beijing. p 2067.
8 Yun SJ, Lee WC. 1994. Characteristics of superoxide dismutase of mulberry leaf. Korean J Breed 26: 389-393
9 Sale FD, Marchesini S, Fishman PH, Berra B. 1984. A sensitive enzymatic assay for determination of cholesterol in lipid extracts. Academic Press Inc., New York. p 347-350.
10 Fujimoto T, Nomura T. 1985. Components of root bark of cudrania tricuspidata 3. Isolation and structure studies on the flavonoids. Planta Med 51: 190-196.   DOI
11 Kim SH, Kim NJ, Choi JS, Park JC. 1993. Determination of flavonoids by HPLC and biological activities from the leaves of cudrania tricuspidata bureau. J Kor Soc Food Sci Nutr 22: 68-72.
12 Yun SJ, Lee WC. 1995. Studies on the utilization of phamacologically active constituents in mulberry: 1. varietal and seasonal variations of flavonol glycoside content in leaves. RDA J Arig Sci 37: 201-205.
13 Lakshmanan MR, Nepokroeff CM, Ness GC, Dugan RE, Potter JW. 1973. Stimulation of insulin of rat liver hydroxymethylglutaryl CoA reductase and cholesterol synthesizing activities. Biochem Biophys Res Commun 50: 704-10.   DOI   ScienceOn
14 Edwards PA, Lan SF, Fogelman AM. 1984. High density lipoproteins and lecithin dispersions increase the activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase by increasing the rate of synthesis and decreasing the rate of degradation of the enzyme. J Biol Chem 259: 8190-8194.
15 Cho MR, Choue RW, Chung SH, Ryu JW. 1998. Effects of silkworm powder on blood glucose and lipid levels in NIDDM (Type II) patients. Kor J Nutr 31: 1139-1150.   과학기술학회마을
16 Purusotam B, Shigetoshi K, Satoshi T, Mineo S, Tsuneo N. 1993. Two new 2-arylbenzofuran derivatives from hypoglycemic activity-bearing fractions of morus insignis. Chem Pharm Bull 41: 1238-1243.   DOI   ScienceOn
17 Feingold KR, Arthur M, Saleh A, Mounzer S, Carl G. 1990. Small intestinal fatty acid synthesis is increased in diabetic rats. Endocrinology 127: 2247-2252.   DOI   ScienceOn
18 Lau AL, Failla ML. 1984. Urinary excretion of zinc, copper and iron in the streptozotocin-diabetic rat. J Nutr 114: 224-223
19 O'Meara NMG, Devery RAM, Owens D, Collins PB, Johnson AH, Tomkin GH. 1990. Cholesterol metabolism in alloxaninduced diabetic rabbits. Diabetes 39: 626-633.   DOI   ScienceOn
20 Choi YS, Lee SY. 1992. Serum cholesterol and 3-hydroxy-3-methylglutaryl coenzyme A reductase. J Korean Soc Food Nutr 21: 580-593.   과학기술학회마을
21 Yun SJ, Lee WC. 1995. Studies on the utilization of pharmacologically active constituents in mulberry 1. Verietal and seasonal variations of flavonol glycoside content in leaves. RDA J Agri Sci 37: 201-205.
22 Goldstein JL, Brown MS. 1997. The low-density lipoprotein pathway and its relation to atherosclerosis. Annu Rev Biochem 46: 897-930.   DOI   ScienceOn
23 Shin DH. 1998. Antioxidative substances in mulberry leaves. J Korean Oil Chemists Soc 16(3): 27-31.   과학기술학회마을
24 Lee JS, Son HS, Maeng YS, Chang YK, Ju JS. 1994. Effects of buckwheat on organ weight, glucose and lipid metabolism in streptozotocin induced diabetic rats. J Kor Nutr 27: 819-827.   과학기술학회마을
25 Huber J, Guder W, Latzin S, Hamprecht B. 1973. The influence of insulin and glucagon on HMG CoA reductase activity in rat liver. Hoppe-Seylers Z Physiol Chem 354: 795-798.
26 Folch JM, Lees M, Stanley GHS. 1957. A simple method for the isolation and purification of total lipids from animal tissue. J Biol Chem 26: 497-509.
27 Kim MH, Kim HY, Kim WK, Kim JY, Kim SH. 2001. Effects of soy oligosaccharides on blood glucose and lipid metabolism in streptozotocin-induced diabetic rats. Kor J Nutr 34: 3-13.
28 Fiordaliso M, Kok N, Desager JP, Goethals F, Deboyser D. Roberfoid M, Delzenne N. 1977. Dietary oligofructose lowers triglycerides, phospholipids and cholesterol in serum and very low density lipoproteins of rats. Lipids 30: 163-167.   DOI   ScienceOn
29 Cha JY, Kim DJ, Cho YS. 2001. Effect of stem bark extract from cudrania tricuspidata on the concentrations of lipid and lipid peroxidation in rats fed a cholesterol diet. Kor J Life Sci 11: 328-334.   과학기술학회마을
30 Chen F, Nakashima N, Kimura I, Kimura M, Asano N, Koya S. 1995. Potentiating effects on pilocarpine-induced saliva secretion by extracts and N-containing sugars derived from mulberry leaves in streptozotocin-diabetic mice. Bio Pharm Bull 18: 1676-1680.   DOI   ScienceOn
31 Bhathena SJ, Avigan J, Schreiner ME. 1974. Effect of insulin on sterol and fatty acid synthesis and hydroxy-methylglutaryl CoA reductase activity in mammalian cells grown in culture. USA Proc Natl Acad Sci 71: 2174-2178.   DOI   ScienceOn
32 Forman S, Estilow ML, Vailenko P. 1996. STZ diabetes alters immunoreactive $\beta$-endorphin levels and pain perception after 8 wk in female rats. Diabetes 35: 1309-1313   DOI   ScienceOn
33 Choi JW, Sohn KH, Kim SH. 1991. The effects of nicotinamide on the serum lipid composition in streptozotocin-induced diabetic rats. J Korean Soc Food Nutr 20: 306-311   과학기술학회마을
34 Lim SJ, Kim YS. 1998. The effect of butanol fraction of polygonatum odoratum with vitamin E on blood glucose levels and lipid peroxidations in streptozotocin-induced diabetic rats. Kore J Nutr 31: 1385-1393.   과학기술학회마을
35 Kim YS, Chung SH, Suh HJ, Chung ST, Cho JS. 1994. Rutin and mineral contents on improved kinds of Korean buckwheat at growing stage. Korean J Food Sci Technol 26: 759-763.   과학기술학회마을
36 Massayasu K, Fu-jun Chen, Noboru N, Ikuho K, Naoki A, Sakuji K. 1995. Anti hyperglycemic effect of N-containing sugars delived from mulberry leaves in streptozotocin-induced diabetic mice. J Traditional Medicine 12: 214-219.
37 Nakayama H, Nakagawa S. 1977. Influence of streptozotocin diabetes on intestinal 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in the rat. Diabetes 26: 439-444.   DOI   ScienceOn
38 Markham KR. 1989. Flavones, flavonols and their glycosides. Methods in Plant Biochemistry 1: 197-235.   DOI
39 Francois B. 1996. Non-insulin dependent diabetes and reverse cholesterol transport. Athersclerosis 124: 39S-42S.   DOI   ScienceOn
40 Sexton WS. 1994. Skeletal muscle vascular transport capacity diabetic rats. Diabetes 43: 225-231   DOI   ScienceOn
41 Kim SY, Lee WC, Kim HB, Kim AJ, Kim SK. 1998. Antihyperlipidemic effects of methanol extracts from mulberry leaves in cholesterol induced hyperlipidemia in rats. J Kor Soc Food Sci Nutr 27: 1217-1222.   과학기술학회마을
42 Tang W, Eisenbrand G. 1992. Chinese drugs of plant origin. Springer-Verlag, Beijing. p 669-696.
43 Steiner G, Chow A, Little JA. 1978. Hypertriglyceridemia associated with deficiency of apolipoprotein C-II. N Eng G Med 298: 1256-1273.
44 Kim SY, Lee WC, Kim HB, Kim AJ, Kim SK. 1998. Antihyperlipidemic effects of methanol extracts from mulberry leaves in cholesterol induced hyperlipidemia in rats. J Kor Soc Food Sci Nutr 27: 1217-1222.   과학기술학회마을
45 Friedwald WT, Levy RI, Fedreicson DS. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without was of the preparative Ultracentrifuge. Clin Chem 18: 499-506.
46 Lee HS, Chung KS, Kim SY, Ryu KS, Lee WC. 1998. Effect of severa sericultural products on blood glucose lowering for alloxan-induced hyperglycemic mice. Korean J Seric Sci 40: 38-42.   과학기술학회마을
47 Sung GB. 1998. Recent mulberry research trend and direction for the improvement. Korean J Seric Sci 40: 180-184.