Browse > Article
http://dx.doi.org/10.3746/jkfn.2002.31.4.672

Inhibitory Effects of Epigallocatechin Gallate on Apoptosis in Human Vascular Endothelial Cells  

Choi, Yean-Jung (Division of Life Sciences, Hallym University)
Choi, Jung-Suk (Division of Life Sciences, Hallym University)
Lee, Se-Hee (Division of Life Sciences, Hallym University)
Lee, Yong-Jin (Division of Life Sciences, Hallym University)
Kang, Jung-Sook (Dept. of Food and Nutrition, Cheju National University)
Kang, Young-Hee (Division of Life Sciences, Hallym University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.31, no.4, 2002 , pp. 672-678 More about this Journal
Abstract
Oxidative stress contributes to cellular injury following clinical and experimental ischemia/reperfusion scenarios. Oxidative injury can induce cellular and nuclear damages that result in apoptotic cell death. We tested the hypothesis that the catechin flavonoid of (-)epigallocatechin gallate, a green tea polyphenol, inhibits hydrogen peroxide ($H_2O$$_2$)-induced apoptosis in human umbilical vein endothelial cells. The effect of apigenin, a flavone found in citrus fruits, on apoptosis parameters was also examined. A 30 min pulse treatment with 0.25 mM $H_2O$$_2$ decreased endothelial cell viability within 24 hrs by > 30% ; this was associated with nuclear condensation and biochemical DNA damage consistent with programmed cell death. In the 0.25 mM $H_2O$$_2$apoptosis model, 50${\mu}{\textrm}{m}$ (-)epigallocatechin gallate markedly increased cell viability with a reduction in the nuclear condensation and DNA fragmentation. In contrast, equimicromolar apigenin increased cell loss with intense DNA laddering, positive nick-end labeling and Hoechst 33258 staining. Thus, polyphenolic (-)epigallocatechin gallate, but not apigenin flavone, qualify as an antioxidant in apoptosis models caused by oxidative stress. Further work is necessary for elucidating the anti-apoptotic mechanisms of polyphenolic catechins.
Keywords
(-)epigallocatechin gallate; catechin; apigenin; flavone; antioxidant; apoptosis; hydrogen peroxide; endothelial cells;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Miller MJ, Angeles FM, Reuter BK, Bobrowski P, Sandoval M. 2001. Dietary antioxidants protect gut epithelial cells from oxidant-induced apoptosis. BMC Complement Altern Med 1: 1-11.   DOI
2 Wang ZY, Huang MT, Lou YR, Xie JG, Reuhl KR, Newmark HL, Ho CT, Yang CS, Conney AH. 1994. Inhibitory effects of black tea, green tea, decaffeinated black tea, and decaffeinated green tea on ultraviolet light-induced skin carcinogenesis in 7,12-dimethylbenz[aJanthracene-initiated SKH-1 mice. Cancer Res 54: 3428-3435.
3 Spencer JP, Schroeter H, Kuhnle G, Srai SK, Tyrrell RM, Hahn U, Rice-Evans C. 2001. Epicatechin and its in vivo metabolite, 3'-O-methyl epicatechin, protect human fibroblasts from oxidative-stress-induced cell death involving caspase-3 activation. Biochem J 354: 493-500.   DOI   ScienceOn
4 Searle J, Kerr JFR, Bishop CJ. 1982. Necrosis and apoptosis: distinct modes of cell death with fundamentally different significance. Pathol Ann 17: 229-259.
5 Choi YT, Jung CH, Lee SR, Bae JH, Baek WK, Suh MH, Park J, Park CW, Suh SI. 2001. The green tea polyphenol (-)-epigallocatechin gallate attenuates beta-amyloid-induced neurotoxicity in cultured hippocampal neurons. Life Sci 70: 603-614.   DOI   ScienceOn
6 Sarafian TA, Bredesen DE. 1994. Is apoptosis mediated by reactive oxygen species? Free Radic Res 21: 1-8.   DOI   ScienceOn
7 Voyta JC, Via DP, Butterfield CE, Zetter BR. 1984. Identification and isolation of endothelial cells based on their increased uptake of acetyl-low density lipoprotein. J Cell Biol 99: 2034-2040.   DOI   ScienceOn
8 Buttke TM, Sandstrom PA. 1993. Oxidative stress as a mediator of apoptosis. Immunol Today 15: 7-13.   DOI   ScienceOn
9 Gardner AM, Xu FH, Fady C, Jacoby FJ, Duffey DC, Tu Y, Lichtenstein A. 1997. Apoptotic vs nonapoptotic cytotoxicity induced by hydrogen peroxide. Free Rad Biol Med 22: 73-83.   DOI   ScienceOn
10 Yang GY, Liao J, Li C, Chung J, Yurkow EJ, Ho CT, Yang CS. 2000. Effect of black and green tea polyphenols on c-jun phosphorylation and $H_2O_2$ production in transformed and nontransformed human bronchial cell lines: possible mechanisms of cell growth inhibition and apoptosis induction. Carcinogenesis 21: 2035-2039.   DOI   ScienceOn
11 Denizot F, Lang R. 1986. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immuno Methods 89: 271-277.   DOI   ScienceOn
12 Yang CS, Maliakal P, Meng X. 2002. Inhibition of carcinogenesis by tea. Annu Rev Pharmacol Toxicol 42: 25-54.   DOI   ScienceOn
13 Lizard G, Petit P, Zamzami N, Vayssiere JL, Mignote B. 1995. The biochemistry of programmed cell death. FASEB J 9: 1277-1287.   DOI
14 Kris-Etherton PM, Keen CL. 2002. Evidence that the antioxidant flavonoids in tea and cocoa are beneficial for cardiovascular health. Curr Opin Lipidol 13: 41-49   DOI   ScienceOn
15 Hockenbery DM, Olivai ZN, Yin XM, Milliman CL, Korsmeyer SJ. 1993. Bcl-2 functions in an antixoidant pathway to prevent apoptosis. Cell 75: 241-251.   DOI   ScienceOn
16 Braca A, Sortino C, Politi M, Morelli I, Mendez J. 2002. Antioxidant activity of flavonoids from Licania licaniaeflora. J Ethnopharmacol 79: 379-381.   DOI   ScienceOn
17 Gavrieli Y, Sherman Y, Ben-Sasson SA. 1992. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 119: 493-501.   DOI   ScienceOn
18 Nijveldt RJ, van Nood E, van Hoorn DE, Boelens PG, van Norren K, van Leeuwen PA. 2001. Flavonoids: a review of probable mechanisms of action and potential applications. Am. J Clin Nutr 74: 418-425.   DOI
19 Wyllie AH, Morris RG, Smith AL, Dunlop D. 1984. Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis. J Pathol 142: 67-77.   DOI
20 Jaffe EA, Nachman RL, Becker CG, Minick CR. 1973. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 52: 2745-2756.   DOI   ScienceOn
21 Arends MJ, Moris RG, Wyllie AH. 1990. Apoptosis: role of the endonuclease. Am J Pathol 136: 593-608.
22 Cotelle N. 2001. Role of flavonoids in oxidative stress. Curr Top Med Chem 1: 569-590.   DOI   ScienceOn
23 Hirano R, Sasamoto W, Matsumoto A, Itakura H, Igarashi O, Kondo K. 2001. Antioxidant ability of various flavonoids against DPPH radicals and LDL oxidation. J Nutr Sci Vitaminol (Tokyo) 47: 357-362.   DOI   ScienceOn
24 Thompson CB. 1995. Apoptosis in the pathogenisis and treatment of disease. Science 267: 1456-1462.   DOI   ScienceOn
25 Sakagami H, Arakawa H, Maeda M, Satoh K, Kadofuku T, Fukuchi K, Gomi K. 2001. Production of hydrogen peroxide and methionine sulfoxide by epigallocatechin gallate and antioxidants. Anticancer Res 21: 2633-2641.
26 Chen NY, Ma WY, Yang CS, Dong Z. 2000. Inhibition of arsenite-induced apoptosis and AP-1 activity by epigallocatechin-3-gallate and theaflavins. J Environ Pathol Toxicol Oncol 19: 287-295.
27 Carson DA, Ribeiro JM. 1993. Apoptosis and disease. Lancet 341: 1251-1254.   DOI   ScienceOn