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http://dx.doi.org/10.3746/jkfn.2002.31.2.333

Antimicrobial Effects of Pinus densiflora Sieb. et Zucc. Ethanol Extract on Listeria monocytogenes  

임용숙 (대구가톨릭대학교 식품공학과)
배만종 (경산대학교 식품과학과)
이신호 (대구가톨릭대학교 식품공학과)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.31, no.2, 2002 , pp. 333-337 More about this Journal
Abstract
To develope food preservative, antimicrobial activities of Pinus densiflora (PD) ethanol extract against Listeria monocytogenes Scott A. Listeria monocytogenes Brie I and Listeria monocytogenes ATCC 19111 were investigated. The ethanol extracts of PD showed strong antimicrobial activities on Listeria monocytogenes. The crude ethanol extracts of PD were further fractionated by ether, ethyl acetate and butanol. The ether fraction from ethanol extract showed the strongest antimicrobial effects on Listeria monocytogenes in tryptic soy broth containing 40 mg/mL ether fractions compared with other fractions. The effect of ethanol extract of pinus densiflora against Listeria monocytogenes culture for growth stage in tryptic soy broth at 35$^{\circ}C$ showed the strongest antimicrobial activites for lag phase. The morphological changes of the cells were observed with transmission electron microscope (TEM) and scanning electron microscope (SEM) and the cells were injured by treatment of 40 mg/mL ethanol extract of Pinus densiflora.
Keywords
Pinus densiflora Sieb. et Zucc; (PD) extract; antimicrobial effects; Listeria monocytogenes;
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