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http://dx.doi.org/10.3746/jkfn.2002.31.1.143

Enhancement of Anticarcinogenic Effect by Combination of Lycii fructus with Vitamin C  

박윤자 (신라대학교 식품영양학과)
김미향 (신라대학교 식품영양학과)
배송자 (신라대학교 식품영양학과)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.31, no.1, 2002 , pp. 143-148 More about this Journal
Abstract
We investigated the cytotoxicity effects of Lycii fructus (LF) on HePG2, MCF7 and C6 cell lines by the MTT assay. We extracted the methanol (LFM) and fractionated to five partition layers. Among partition layers, the ethylether partition layer (LFMEE) was showed the strongest cytotoxic effects on all cancer cell lines. The hexane partition layer (LFMH) also was showed significant cytotoxic activities on Hela and MCF-7 cell lines. We also determined the induction of intracellular quinone reductase (QR) activity on HepG2 cells. Among various partition layers of Lycii fructus; LFMH was showed the most effective such induced effect such as 1.85 to the control value of 1.0. And we also determined the enhancement of anticarcinogenic effect by combination of Lycii fructus with vitamin C on all cell lines. These results suggest that potentially useful anticarcinegenic chemicals could be isolated from LFMEE and LFMH of the Lycii frutus and also we found the enhanced effect by the combination of various partition layers of LFM with vitamin C.
Keywords
cytotoxicity; quinone reductase(QR); Lycii fructus; cancer cell lines;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Park, H.J. : Induction of quinone reductase and its regulatory mechanism at the transcriptional level by Scutellaria baicalensis. Ph.D. Dissertation, Yonsei University (1998)
2 Haung, W.I and Oh, S.K. : Effects of petroleum ether extract of ginseng root on same enzyme activity in guman colon cancer cells. Korean J. Ginseng Sci., 15, 153-166 (1984)
3 Doll, R. and Peto, R. : The causes of cancer-Quantitative estimate of aboidable risks of cancer in the united states today. J. Natl. Cancer Inst., 66, 1191-1196 (1981)
4 The Korean society of nutrition : Recommended dietary allowances for Koreans. 7th revision (2000)
5 Giampieti, A. : Drug-mediated increase of tumor immunogenicity in vitro fro a new approach to experimental cancer immunotherapy. Cancer Res., 41, 681-687 (1981)
6 Suzuki, H., Ishigaki, A. and Hara, Y. : Long-term effect of a trace amount of tea catechins with perilla oil on the plasma lipids in mice. Int. J. Vitam. Nutr. Res., 68, 272-274 (1998)
7 Wakabayashi, K., Nagao, M., Esumi, H. and Sugimura, T. : Food-derived mutagens and carcinogens. Cancer Res., 52, 2092-2096 (1992)
8 Choi, O.J. : Component and using of medical plants. Ilwoulseogak, Seoul, p.632-634 (1999)
9 Carmichael, J., De Graff, W.G., Gazder, A.F., Minna, J.D. and Mitchell, J.B. : Evalution of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res., 47, 936-942 (1987)
10 Han, E.J., Roh, S.B. and Bae, S.J. : Cytotoxicity of Daucus carota L. on various cancer cells. J. Korean Soc. Food Sci. Nutr., 29, 153-160 (2000)   과학기술학회마을
11 Sohn, H.E., Lee, J.Y., Kim, D.C. and Hwang, W.I. : Enhancement of anticancer activity by combination of garlic (Allium sativum) extract and vitamin C. J. Korean Soc. Food Sci. Nutr., 30, 372-376 (2001)   과학기술학회마을
12 Cooper, G.M. : Elements of human cancer. Jones and Bantlett publishers, Inc., Boston, p.31 (1992)
13 Hwang, W.I., Lee, S.D. and Oh, S.K. : A study on the pharmacological activites of Lorean medicinal herbs, mainly on the antitumor activities (in Korean). Korean Biochem. J., 15, 205-219 (1982)
14 Han, E.J., Roh, S.B. and Bae, S.J. : Effects of quinone reductase induction and cytotoxicity of the Angelica radix extracts. J. Korean Soc. Food Sci. Nutr., 29, 147-152 (2000)   과학기술학회마을
15 Michael, C.A., Dominic, A.S., Anne, M., Miriam, L.H., Maciej,J.C., Donald, L.F., Betty, J.A., Joseph, G.M., Robert, H.S. and Michael, R.B. : Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res., 48, 589-601 (1988)
16 Shim, S.M., Choi, S.W. and Bae, S.J. : Effects of Punica grantum L. fractions on quinone reductase induction and growth inhibition on several cancer cells. J. Korean Soc. Food Sci. Nutr., 30, 80-85 (2001)   과학기술학회마을
17 Weinstein, I.B. : Cancer prevention : Recent progress and future opoportunities. Cancer Res., 51, 5080-5085 (1991)
18 Carmichael, J., De Graff, W.G., Gazder, A.F., Minna, J.D. and Mitchell, J.B. : Evalution of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res., 47, 936-942 (1987)   DOI   ScienceOn
19 Rew, T.H. : Relation of food, nutrition and cancer. J. Korean Soc. Food Sci. Nutr., 14, 305-401 (1985)
20 Kim, Y.S., Lee, B.E., Kim, K.J., Lee, Y.T., Cho, K.B. and Chung, Y.C. : Antitumor and immunomodulatory activities of the P. grandiflorum cultivated for more than 20 years. Yakhak Hoeji, 42, 382-387 (1998)   과학기술학회마을