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http://dx.doi.org/10.13103/JFHS.2022.37.3.143

Modification and Validation of an Analytical Method for Dieckol in Ecklonia Stolonifera Extract  

Han, Xionggao (Department of Food Biotechnology and Environmental Science, Kangwon National University)
Choi, Sun-Il (Department of Food Biotechnology and Environmental Science, Kangwon National University)
Men, Xiao (Department of Food Biotechnology and Environmental Science, Kangwon National University)
Lee, Se-jeong (Department of Food Biotechnology and Environmental Science, Kangwon National University)
Oh, Geon (Department of Food Biotechnology and Environmental Science, Kangwon National University)
Jin, Heegu (Department of Food Science and Biotechnology, College of Life Science, CHA University)
Oh, Hyun-Ji (Department of Food Science and Biotechnology, College of Life Science, CHA University)
Kim, Eunjin (Naturalway Co. Ltd)
Kim, Jongwook (Naturalway Co. Ltd)
Lee, Boo-Yong (Department of Food Science and Biotechnology, College of Life Science, CHA University)
Lee, Ok-Hwan (Department of Food Biotechnology and Environmental Science, Kangwon National University)
Publication Information
Journal of Food Hygiene and Safety / v.37, no.3, 2022 , pp. 143-148 More about this Journal
Abstract
This study was to investigate an analytical method for determining dieckol content in Ecklonia stolonifera extract. According to the guidelines of International Conference on Harmonization. Method validation was performed by measuring the specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ) of dieckol using high-performance liquid chromatography-photodiode array. The results showed that the correlation coefficient of calibration curve (R2) for dieckol was 0.9997. The LOD and LOQ for dieckol were 0.18 and 0.56 ㎍/mL, respectively. The intra- and inter-day precision values of dieckol were approximately 1.58-4.39% and 1.37-4.64%, respectively. Moreover, intra- and inter-day accuracies of dieckol were approximately 96.91-102.33% and 98.41-105.71%, respectively. Thus, we successfully validated the analytical method for estimating dieckol content in E. stolonifera extract.
Keywords
Ecklonia stolonifera; Dieckol; HPLC-PDA; Method validation;
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