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http://dx.doi.org/10.13103/JFHS.2020.35.3.284

Immunostimulatory and Bone Health-Promoting Activities of Litsea japonica Fruit Extract  

Park, Sung-Jin (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University)
Kang, Jun-Chul (Haram Central Research Institute Co., Ltd)
Lee, Da-young (Haram Central Research Institute Co., Ltd)
Cho, Ju-Hyun (Haram Central Research Institute Co., Ltd)
Yoon, Min-Ho (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University)
Publication Information
Journal of Food Hygiene and Safety / v.35, no.3, 2020 , pp. 284-289 More about this Journal
Abstract
This study was performed with hot water extract (LJF-W) and 70% ethanol extract (LJF-70E) of Litsea japonica fruit to investigate the immunostimulatory activity and bone health-promoting effect of L. japonica fruit. The production of pro-inflammatory mediator (nitric oxide) and pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) in murine macrophage RAW264.7 cells were estimated to examine the immunostimulatory activity of the fruit extracts. The immunostimulatory activity of LJF-W was higher than that of positive control (geinsenosides). However, there was no effect in LJF-70E. Futhermore, both LJF-W and LJF-70E appeared to stimulate the proliferation of MC3T3-E1 cells, proving the effect as a bone health agent. From this result it could be presumed that L. japonica fruit extracts not only stimulate the immune system, but also the ability to promote bone health.
Keywords
Litsea japonica fruit extract; Immuno-stimulation; Bone-health;
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1 Celeste, A.J, Iannazzi, J.A., Taylor, R.C., Hewick, R.M., Rosen, V., Wang, E.A., Wozney, J.M., Identification of transforming growth factor beta family members present in boneinductive protein purified from bovine bone. Proc. Natl. Acad. Sci. USA, 87, 9843-9847 (1990).   DOI
2 Suda, T., Takahashi, N., Martin, T.J., Modulation of osteoclast differentiation. Endocr. Rev., 13(1), 66-80 (1992).
3 Mitsunaga, T., Doi, T., Kondo, Y., Abe, I., Color development of proanthocyanidins in vanillin-hydrochloric acid reaction. J. Wood Sci., 44, 125-130 (1998).   DOI
4 Zhang, L., Cheng, Y.X., Liu, A.L., Wang, H.D., Wang, Y.L., Du, G.H., Antioxidant, anti-inflammatory and anti-influenza properties of components from Chaenomeles speciosa. Molecules, 15(11), 8507-8517 (2010).   DOI
5 Choung, M.G., Lim, J.D., Antioxidant, anticancer and immune activation of anthocyanin fraction from Rubus coreanus Miquel fruits (Bokbunja). Korean J. Medicinal Crop. Sci., 20(4), 259-269 (2012).   DOI
6 Kim, H.S., Kang, J.S., Preparation and characteristics of bread by medicinal herb composites with immunostimulation activity. J. Korean Soc. Food Sci. Nutr., 37, 109-116 (2008).   DOI
7 Cho, C.W., Han, C.J., Rhee, Y.K., Lee, Y.C., Shin, K.S., Shin, J.S., Lee, K.T., Hong, H.D., Cheonggukjang polysaccharides enhance immune activities and prevent cyclophosphamide- induced immunosuppression. Int. J. Biol. Macromol., 72, 519-525 (2015).   DOI
8 Yu, A.R., Park, H.Y., Kim, Y.S., Ha, S.K., Hong, H.D., Choi, H.D., Immuno-enhancing effect of seed extracts on a RAW 264.7 macrophage cell line. J. Korean Soc. Food Sci. Nutr., 41, 1671-1676 (2012).   DOI
9 Ducy, P., Zhang, R., Geoffroy, V., Ridall, A.L., Karsenty, G., Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell, 89(5), 747-754 (1997).   DOI
10 Yoon, W.J., Composition for immunity stimulatory activity using a fruit extract of Litsea japonica. Korea Patent NO. 1020170084331 (2017).
11 Cha, J.H., Kim, Y.S., Lee, E.M., Effects of Prunellae spica water extract on immune response in macrophage cells. J. Korean Obstet. Gynecol., 23, 91-100 (2010).   DOI
12 Aloia, J.F., Cohr, S.H., Vaswani, A., Yeh, J.K., Yuen, K., Ellis, K., Risk factors for postmenopausal osteoporosis. Am. J. Med., 78, 95-100 (1985).
13 Jimenez-Perez, N.C., Lorea-Hernandez, F.G., Jankowski, C.K., Reyes-Chilpa, R., Essential oils in mexican bays (Litsea spp., Lauraceae): Taxonomic assortment and ethnobotanical implications. Econ. Bot., 65, 178-189 (2011).   DOI
14 Min, B.S., Lee, S.Y., Kim, J.H., Kwon, O.K., Park, B.Y., An, R.B., Lee J.K., Moon, H.I., Kim, T.J., Kim, Y.H., Joung, H., Lee, H.K., Lactones from the leaves of the Litsea japonica and their anti-complement activity. J. Nat. Prod., 66, 1388-1390 (2003).   DOI
15 Takeda, K., Sakurawi, K. Ishii, H., Components of the Lauraceae family-l. New lactonic compounds from Litsea japonica. Tetrahedron, 28, 3757-3766 (1972).   DOI
16 Kandulski, A., Selgrad, M., Malfertheiner, P., Helicobacter pylori infection: a clinical overview. Dig Liver Dis., 40, 619-626 (2008).   DOI
17 Jang, M., Lim, T.G., Hong, H.D., Rhee, Y.K., Kim, K.T., Lee, E.J., Lee, J.H., Lee, Y.J., Kim, Y.B., Cho, C.W., Immunostimulatory activities of a high molecular weight fraction from Cynanchum wilfordii Radix obtained by ultrafiltration. Korean J. Food Sci. Technol., 48(3), 268-274 (2016).   DOI
18 Johnston, R.B. Jr., Keele, B.B. Jr., Misra, H.P., Lehmeyer, J.E., Webb, L.S., Baehner, R.L., RaJagopalan, K.V., The role of superoxide anion generation in phagocytic bactericidal activity. Studies with normal and chronic granulomatous disease leukocytes. J. Clin. Invest., 55(6), 1357-1372 (1975).   DOI
19 Abo, T., Kawamura, T., Watanabe, H., Immunologic states of autoimmune diseases. Immunol. Res., 33(1), 23-34 (2005).   DOI
20 Nathan, C.F., Secretory products of macrophages. J. Clin. Invest., 79, 319-326 (1987).   DOI
21 Langermans, J.A., Van der Hulst, M.E., Nibbering, P.H., Hiemstra, P.S., Fransen, L., Van Furth, R., IFN-gammainduced L-arginine-dependent toxoplasmastatic activity in murine peritoneal macrophages is mediated by endogenous tumor necrosis factor-alpha. J. Immunol., 148(2), 568-574 (1992).
22 Henry, Y., Ducrocq, C., Drapier, J.C., Servent, D., Pellat, .C., Guissani, A., Nitric oxide, a biological effector. Electron paramagnetic resonance detection of nitrosyl-iron-protein complexes in whole cells. Eur. Biophys. J., 20(1), 1-15 (1991).   DOI
23 Jin, M., Chung, K.S., Enhancement of immune responses by a water soluble proteoglycan, lepidan from Lentinus lepideus. Yakhak Hoeji, 43(5), 635-641 (1999).
24 Herberman, R.B., Ortaldo, J.R., Natural killer cell: Their roles in defenses against disease. Science, 214, 24-30 (1981).   DOI
25 Boisvert, C.A., The pelvic fin and girdle of Panderichthys and the origin of tetrapod locomotion. Nature, 438, 1145-1147 (2005).   DOI
26 Hibbs, J.B. Jr., Taintor, R.R., Vavrin, Z., Rachlin, E.M., Nitric oxide: A cytotoxic activated macrophage effector molecule. Biochem. Bioph. Res. Co., 157, 87-94 (1998).   DOI
27 Teitelbaum, S.L., Bone resorption by osteoclasts. Science, 289, 1504-1508 (2000).   DOI
28 Knothe Tate, M.L., Adamson, J.R., Tami, A.E., Bauer, T.W., The osteocyte. Int. J. Biochem. Cell Biol., 36(1), 1-8 (2004).   DOI