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http://dx.doi.org/10.13103/JFHS.2020.35.3.271

Prevalence and Characterization of Virulence Genes in Escherichia coli Isolated from Diarrheic Piglets in Korea  

Kim, Sung Jae (Department of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University)
Jung, Woo Kyung (Department of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University)
Hong, Joonbae (Food & Microbiology Team, Test & Research Department, Consumer Safety Center, Korea consumer Agency)
Yang, Soo-Jin (Department of Animal Science and Technology, Chung-Ang University)
Park, Yong Ho (Department of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University)
Park, Kun Taek (Department of Biotechnology, Inje University)
Publication Information
Journal of Food Hygiene and Safety / v.35, no.3, 2020 , pp. 271-278 More about this Journal
Abstract
Enterotoxigenic Escherichia coli is one of the major causative infectious agents of diarrhea in newborn and post-weaning pigs and leads to a large economic loss worldwide. However, there is limited information on the distribution and characterization of virulence genes in E. coli isolated from diarrheic piglets, which also applies to the current status of pig farms in Korea. To investigate the prevalence and characterization of virulence genes in E. coli related to diarrhea in piglets, the rectal swab samples of diarrheic piglets (aged 2 d to 6 w) were collected from 163 farms between 2013 and 2016. Five to 10 individual swab samples from the same farm were pooled and cultured on MacConkey agar plates, and E. coli were identified using the API 32E system. Three sets of multiplex PCRs were used to detect 13 E. coli virulence genes. As a result, a total of 172 E. coli isolates encoding one or more of the virulence genes were identified. Among them, the prevalence of individual virulence gene was as follows, (1) fimbrial adhesins (43.0%): F4 (16.9%), F5 (4.1%), F6 (1.7%), F18 (21.5%), and F41 (3.5%); (2) toxins (90.1%): LT (19.2%), STa (20.9%), STb (25.6%), Stx2e (15.1%), EAST1 (48.3%); and (3) non-fimbrial adhesin (19.6%): EAE (14.0%), AIDA-1 (11.6%) and PAA (8.7%), respectively. Taken together, various pathotypes and virotypes of E. coli were identified in diarrheic piglets. These results suggest a broad array of virulence genes is associated with coliform diarrhea in piglets in Korea.
Keywords
Diarrheic piglet; E. coli: Prevalence; Virulence factor;
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