Browse > Article
http://dx.doi.org/10.13103/JFHS.2020.35.3.219

Distribution and Toxin Gene Characteristic of Bacillus cereus Isolated from Foods in Busan  

Park, So-hyun (Microbiology Team, Busan Metropolitan City Institute of Health & Environment)
Gwon, Wi-Gyeong (Microbiology Team, Busan Metropolitan City Institute of Health & Environment)
Lee, In-sook (Microbiology Team, Busan Metropolitan City Institute of Health & Environment)
Kim, Eun-ju (Microbiology Team, Busan Metropolitan City Institute of Health & Environment)
Hwang, Su-jeong (Microbiology Team, Busan Metropolitan City Institute of Health & Environment)
Koo, Hee-soo (Microbiology Team, Busan Metropolitan City Institute of Health & Environment)
Na, Young-ran (Microbiology Team, Busan Metropolitan City Institute of Health & Environment)
Kim, Byung-jun (Microbiology Team, Busan Metropolitan City Institute of Health & Environment)
Park, Eun-hee (Microbiology Team, Busan Metropolitan City Institute of Health & Environment)
Lee, Mee-ok (Microbiology Team, Busan Metropolitan City Institute of Health & Environment)
Publication Information
Journal of Food Hygiene and Safety / v.35, no.3, 2020 , pp. 219-224 More about this Journal
Abstract
The purpose of this study was to provide basic data necessary for the prevention of food poisoning and safe food management. We examined 872 food samples for B. cereus in accordance with the MFDS Food Code and investigated characteristics of their harboring toxin genes. We detected and isolated 113 strains of B. cereus from 78 food samples (8.9%), and the average detection level was 48 CFU/g. B. cereus isolates carried at least 1 toxin gene among the emetic toxins and 5 enterotoxin genes. The toxin gene profiles of B. cereus were classified into 18 different types of isolates showing genetic diversity. Among the strains, 34 (30.1%) had all 5 enterotoxin genes (Cytk-nheA-entFM-bceT-hblC), accounting for the highest percentage. The entFM and nheA genes were major enterotoxin genes, while the emetic toxin gene, CER, was the least detected in B. cereus isolated from food samples.
Keywords
Bacillus cereus; Enterotoxin; Emetic toxin; Toxin gene profile;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Kim, J.Y., Kwon, K.I., Ha, S.Y., Hong, C.H., Changes of contamination level of Listeria spp. during the processing environment in kimbab restaurants. J. Food Hyg. Saf., 20, 232-236 (2005).
2 Bahk, G.J., Chun, S.J., Park, K.H., Hong, C.H., Kim, J.W., Survey on the foodborne illness experience and awareness of food safety practice among Korean consumers. J. Food Hyg. Saf., 18, 139-145 (2003).
3 Choi, J.W., Park, S.Y., Yeon, J.H., Lee, M.J., Chung, D.H., Lee, K.H., Kim, M.G., Lee, D.H., Kim, K.S., Ha, S.D., Microbial contamination levels of fresh vegetables distributed in markets. J. Food Hyg. Saf., 20, 43-47 (2005).
4 Ministry of food and drug safety, (2019, October 31). Available from, http://www.mfds.go.kr/e-stat/index.do.
5 Cartwright, E.J., Jackson, K.A., Johnson, S.D., Graves, L.M., Silk, B.J., Mahon, B.E., Listeriosis outbreaks and associated food vehicles, United States, 1998-2008. Emerg Infect Dis., 19, 1-9 (2013).   DOI
6 Kim, J.B., Kim, J.M., Cho, S.H., Oh, H.S., Choi, N.J., Oh, D.H., Toxin genes profiles and toxin production ability of Bacillus cereus isolated from clinical and food samples. J. Food Sci., 76, 25-29 (2011).
7 Kim, T.S., Kim, M.J., Kang, Y.M., Molecular characterization and toxin profile of Bacillus cereus strains isolated from ready-to-eat foods. Korean J. Food Sci. Technol., 46, 334-340 (2014).   DOI
8 Choi, S.K., Lee, M.S., Lee, K.H., Lim, D.S., Lee, K.H., Kim, C.H., Changes in quality of hamburger and sandwich during storage under simulated temperature and time. Korean J. Food Sci. Ani. Resour., 18, 27-34 (1998).
9 Kim, S.J., Jung, J.H., Tahk, H.M., Baek, S.Y., Lee, S.Y., Effect of factors on the sporulation of Bacillus cereus and their thermal resistance. J. Food Hyg. Saf., 24, 256-261 (2009).
10 Ankolekar, C., Rahmati, T., Labbe, R.G., Detection of toxigenic Bacillus cereus and Bacillus thuringiensis spore in U.S. rice. Int. J. Food Microbiol., 128, 460-466 (2008).   DOI
11 Anwarul, H., Nicholas, J.R., Phenotypic and genotypic characterisation of Bacillus cereus isolates Bangladeshi rice. Int. J. Food Microbiol., 98, 23-24 (2005).   DOI
12 Valero, M., Hernandez-Herrero L.A., Fernandez, P.S., Salmeron, M.C., Characterization of Bacillus cereus isolates from fresh vegetables and refrigerated minimally processed foods by biochemical and physiological tests. Int. J. Food Microbiol., 19, 491-499 (2002).   DOI
13 Ministry of Food and Drug Safety, (2019, October 31). Food Poisonning Statistics, https://www.foodsafetykorea.go.kr/main.do.
14 EFSA. Opinion of the scientific panel on biological hazards on Bacillus cereus and other Bacillus spp. in foodstuffs. EFSA J., 75, 1-48 (2005).
15 Kim, J.B., Kim, J.M., Cho, S.H., Oh, H.S., Choi, N.J., Oh, D.H., Toxin genes profiles and toxin production ability of Bacillus cereus isolated from clinical and food samples. J. Food Sci., 76, 25-29 (2011).
16 Kotiranta, A., Lounatmaa, K., Haapasalo, M., Epidemiology and pathogenesis of Bacillus cereus infections. Microbes Infect., 2, 189-198 (2000).   DOI
17 Drobniewski, F.A., Bacillus cereus and related species. J. Clin. Microbiol., 6, 324-338 (1993).   DOI
18 Korea Centers for Disease Control and Prevention, National Research Institute of health, 2017, Infection Disease Laboratory Diagnosis: Disease-Specific Protocol, Cheongju, Korea, pp. 45-47.
19 Ehling-Schulz, M., Guinebretiere, M.H., Monthan, A., Berge, O., Fricker, M., Svensson, B., Toxin gene profiling of enterotoxin and emetic Bacillus cereus. FEMS Microbiol. Lett., 260, 232-240 (2006).   DOI
20 Korea Centers for Disease Control and Prevention, National Research Institute of health, 2019, Guidelines for the management of waterborne and food poisoning infections, Cheongju, Korea, pp.273-279.
21 Ministry of Food and Drug Safety, 2019, Food Code, Cheongju, Korea.
22 Sung, G.H., Hwang, I.Y., Park, S.H., Park, S.H., Kim, H.J., Lee, J.H., Min, S.K., Study on microbiological safety of simple processed agricultural products. Korean J. Food Sci. Technol., 49, 599-604 (2017).   DOI
23 Kang, T.M., Cho, S.K., Park, J.Y., Song, K.B., Chung, M.S., Park, J.H., Analysis of microbial contamination of sprouts and fresh-cut salads in a market. Korean J. Food Sci. Technol., 43, 490-494 (2011).   DOI
24 Choi, J.H., Park, J.Y., Lim, E.G., Choi, M.K., Kim, J.S., Choi, G.B., Jeong, S.G., Hahm, Y.S., An investigation of microbial contamination of side dishes sold at traditional market and supermarket in Ulsan. J. Food Hyg. Saf., 27, 87-95 (2012).   DOI
25 Hwang, I.S., Jang, M.R., Kim, O.H., Lee, S.D., Park, Y.A., Choi, B.C., Lee, K.A., Kim, L.L., Kim, D.K., Jung, A.H., Oh, Y.H., Kim, J.H., Jung, K., Contents of sodium, potassium and food-borne pathogens contamination of side dishes distributed in Seoul area. J. Food Hyg. Saf., 32, 35-41 (2017).   DOI
26 Chon, J.W., Kim, J.H., Lee, S.J., Hyeon, J.Y., Song, K.Y., Park, C., Seo, K.H., Prevalence, phenotypic traits and molecular characterization of emetic toxin-producing Bacillus cereus strains isolated from human stools in Korea. J. Appl. Microbiol., 112, 1042-1049 (2012).   DOI
27 Gilbert, R.J., Humphrey, T.J., 1998. Food-borne bacterial gastroenteritis. In : Hausler W. J., Sussman M(eds), Topley and Wilson's Microbiology and microbial infection 9th edn. vol. 3. Bacterial. Hodder Arnond, London, UK.
28 Granum, P.E., Lund, T., Bacillus cereus and its food poisoning toxins. FEMS Microbiol. Lett., 157, 223-228 (1997).   DOI
29 Vassileva, M., Torii, K., Oshimoto, M., Okamoto, A., Agata, N., Yamada, K., Hasegawa, T., Ohta, M., A new phylogenetic cluster of cereulide-producing Bacillus cereus strains. J. Clin Microbiol., 45, 1274-1277 (2007).   DOI
30 Stenfors Arnesen, L.P., Fagerlund, A., Granum, P.E., From soil to gut: Bacillus cereus and its food poisoning toxins. FEMS Microbiol., 32, 579-606 (2008).   DOI
31 Ngamwongsatit, P., Buasri, W., Pianariyanon, P., Pulsrikarn, C., Ohba M., Assavanig, A., Panbangred, W., Broad distribution of enterotoxin genes (hblCDA, nheABC, cytK, and entFM) among Bacillus thuringiensis and Bacillus cereus as shown by novel primers. Int. J. Food Microbiol., 121, 352-356 (2008).   DOI