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http://dx.doi.org/10.13103/JFHS.2019.34.6.583

Effect of Light-Induced ROS Generation Unit on Inactivation of Foodborne Pathogenic Bacteria in Water  

Choi, Jaehyuk (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institute of Agricultural Sciences (NIAS), Rural Development Administration (RDA))
Kim, Dawoon (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institute of Agricultural Sciences (NIAS), Rural Development Administration (RDA))
Jung, Kyu-Seok (Agricultural Resource Research Institute, Gyeonggi-do Agricultural Research and Extension Services)
Roh, Eunjung (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institute of Agricultural Sciences (NIAS), Rural Development Administration (RDA))
Ryu, Kyoung-Yul (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institute of Agricultural Sciences (NIAS), Rural Development Administration (RDA))
Ryu, Jae-Gee (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institute of Agricultural Sciences (NIAS), Rural Development Administration (RDA))
Publication Information
Journal of Food Hygiene and Safety / v.34, no.6, 2019 , pp. 583-590 More about this Journal
Abstract
As the consumption of fresh fruits and vegetables increases, food poisoning caused by foodborne pathogen contamination is not decreasing. To prevent the contamination of produce, a quick and easy, low-cost, environmentally-safe disinfection method that does not affect produce freshness or quality is needed. This study demonstrates a new-concept, circulating-water disinfection system that purifies water by using newly developed 'LED-PS (photosensitizer)-induced ROS generation unit'. Using various types of LED-PS induced ROS generation units, we investigated the conditions for reducing the density of various pathogenic bacteria by more than 3 log CFU / mL in 1 hour. The major operational factors affecting the density reduction of the LED-PS-induced ROS generation unit were analyzed. Depending on bacteria species, the density reduction rate was varied. The effect of the units on reducing the density of Bacillus cereus and Pectobacterium carotovorum subsp. carotovorum was high, but the effect on foodborne bacteria such as Escherichia coli was relatively low. In this circulating water disinfection system, the density reduction effect tended to increase as the flow rate increased and the initial bacterial density decreased. As the amount of PS absorbed beads increased, the density reduction effect increased exponentially in some bacteria. Model 3280, a double cylindrical unit connecting two single cylindrical units, could completely sterilize more than 3 log CFU/mL of B. cereus and P. carotovorum subsp. carotovorum in 30 minutes of LED irradiation.
Keywords
Reactive oxygen species; Foodborne pathogens; Photosensitizer; Disinfection; Light induced ROS generator;
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