[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.13103/JFHS.2019.34.3.277

Comparison of Loop-mediated Isothermal Amplification and Korea Standard Food Codex (KFSC) Method for Detection of Salmonella Typhimurium, Listeria monocytogenes Artificially Inoculated in Yuk-hwe and Yuk-sashimi

Gwak, Seung-Hae (Department of Food and Nutrition, Kookmin University)
Lee, So-Young (Department of Food and Nutrition, Kookmin University)
Kim, Jin-Hee (Department of Food and Nutrition, Kookmin University)
Oh, Se-Wook (Department of Food and Nutrition, Kookmin University)

Publication Information

Journal of Food Hygiene and Safety / v.34, no.3, 2019 , pp. 277-282 More about this Journal

Abstract

The object of this study is to compare the performance of the 3M Molecular Detection Assay 2 (3M MDA 2) and the Korea Standard Food Codex (KSFC) Method (i.e., isolation media and real-time PCR) in detecting Salmonella Typhimurium and Listeria monocytogenes in traditional Korean foods. Yuk-hwe and Yuk-sashimi (types of raw beef dishes) were artificially inoculated with $10^0-10^4CFU/25g$ of L. monocytogenes and S. Typhimurium. Citrobacter freundii and Listeria innocua were used as competitive microflora. After enrichment, the samples were analyzed using 3M MDA 2 and real-time PCR. All samples inoculated at concentrations of $10^0-10^4CFU/25g$ without competitive microflora were positive for S. Typhimurium and L. monocytogenes, as detected by 3M MDA 2 and Korea Standard Food Codex (KFSC) Method. In addition, part of the samples were positive for the presence of C. freundii and L. innocua. The 3M MDA 2 - Salmonella and Korea Standard Food Codex (KFSC) Method showed similar detection performances in Yuk-hwe and Yuk-sashimi. The 3M MDA 2 method for Salmonella and Listeria, which is a LAMP-based technology, can be used for rapid detection of S. Typhimurium and L. monocytogenes in raw beef. LAMP bioluminescence assays provide results on the subsequent day and are simple to use compared with the Korea Standard Food Codex (KFSC) Method, particularly in terms of DNA preparation.

Keywords

3M Molecular Detection Assay 2; Korea Standard Food Codex; Rapid detection; Validation; S. Typhimurium; L. monocytogenes;

Citations & Related Records

Times Cited By KSCI : 1 (Citation Analysis)

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