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http://dx.doi.org/10.13103/JFHS.2017.32.5.418

Development of an Analytical Method for the Determination of Dexamethasone in Bovine Milk Using Liquid Chromatography Coupled to Tandem Mass Spectrometry  

Cha, Chun-Nam (Engineering Research Institute and Department of Industrial Systems Engineering, Gyeongsang National University)
Park, Eun-Kee (Medical Humanities and Social Medicine, College of Medicine, Kosin University)
Yoo, Chang-Yeul (Department of Smart Information Convergence, Gyeongnam Provincial Namhae College)
Lee, Sung Joong (Department of Chemistry and Research Institute of Live Science, Gyeongsang National University)
Son, Song-Ee (Institute of Animal Medicine and College of Veterinary Medicine, Gyeongsang National University)
Kim, Suk (Institute of Animal Medicine and College of Veterinary Medicine, Gyeongsang National University)
Lee, Hu-Jang (Institute of Animal Medicine and College of Veterinary Medicine, Gyeongsang National University)
Publication Information
Journal of Food Hygiene and Safety / v.32, no.5, 2017 , pp. 418-423 More about this Journal
Abstract
An analytical method for the determination of dexamethasone (DM) in bovine milk samples was developed and validated using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Milk samples were extracted by the liquid-liquid extraction based on acetonitrile. The chromatographic separation was achieved on a reverse phase $C_{18}$ column with gradient elution using a mobile phase of 0.1% formic acid in 95% acetonitrile. The procedure was validated according to the Ministry of Food and Drug Safety guideline determining accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ). Mean recoveries of DM from spiked milk samples (25, 125, and 1,250 ng/mL) were 98.9-109.6%, and the relative standard deviation was between 1.7 and 4.4%. Linearity in concentration range of 12.5-1,250 ng/mL was obtained with the correlation coefficient ($r^2$) of 0.9997. LOD and LOQ for the investigated DM were 0.15 and 0.5 ng/mL depending on milk samples, respectively. This method was reliable, sensitive, economical and suitable for routine monitoring of DM residues in bovine milk.
Keywords
Dexamethasone; Bovine Milk; LC-MS/MS;
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Times Cited By KSCI : 2  (Citation Analysis)
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