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http://dx.doi.org/10.13103/JFHS.2016.31.5.342

Comparative Study of Change in Salmonella Enteritidis and Salmonella Typhimurium Populations in Egg white and Yolk  

Moon, Hye Jin (Department of Food and Nutrition, Kyung Hee University)
Lim, Jeong Gyu (Department of Food and Nutrition, Kyung Hee University)
Yoon, Ki Sun (Department of Food and Nutrition, Kyung Hee University)
Publication Information
Journal of Food Hygiene and Safety / v.31, no.5, 2016 , pp. 342-348 More about this Journal
Abstract
The objective of this study was to compare the change of S. Enteritidis with S. Typhimurium populations in liquid egg products. S. Enteritidis or S. Typhimurium was inoculated into egg white and egg yolk and stored at 8, 10, 15, 25, and $35^{\circ}C$, respectively. In egg white, no growth of S. Enteritidis and S. Typhimurium was observed at 8, 10, 15, and $35^{\circ}C$, while both S. Enteritidis and S. Typhimurium in egg white stored grew more than 1 log CFU/ml after 50 hours storage at $25^{\circ}C$. In egg yolk, there was no growth of S. Enteritidis and S. Typhimurium at $8^{\circ}C$ but growth of both strains was observed at 10, 15, 25, and $35^{\circ}C$. Since growth of S. Enteritidis and S. Typhimurium was only observed in egg yolk, primary growth models for both strains were developed using modified Gompertz equation and then secondary models for lag time (LT), specific growth rate (SGR), and maximum population density (MPD) were developed as a function of temperature. At all temperatures, more rapid growth of S. Enteritidis than S. Typhimurium was observed in egg yolk, indicating the greater risk of S. Enteritidis than S. Typhimurium in egg products. In conclusion, the results indicate that temperature control less than $8^{\circ}C$ is very important to ensure safety of liquid egg products, especially liquid egg yolk.
Keywords
Salmonella Enteritidis; Salmonella Typhimurium; egg white; egg yolk;
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1 Kim, Y.J., Chon, J.W., Lim, J.S., Song, B.R., Seo, K.H., Heo, E.J., Moon, J.S.: Contamination at Egg Processing Plants in South Korea:Prevalence, Antibiotic Resistance, and Epidemiological Tracing by Rep-PCR Fingerprinting. J. Food Sci., 80, 759-764 (2015).   DOI
2 Na, Y.J.: Risk assessment and control of Salmonella Enteritidis on ready-to-eat(RTE) quail eggs products. M.S. Thesis, Kyunghee University, (2014).
3 Seo, K.Y.: Development of Predictive Model for Growth of Salmonella Typhimurium in Kimbab. M.S. Thesis, Chungang University, (2008).
4 Lee, H.J.: Predictive Model for growth of salmonella Enteritidis in fresh-cut-cabbage. M.S. Thesis, Hanyang University, (2008).
5 Gibson, A.M., Bratchell, N., Roberts, T.A.: The effect of sodium chloride and temperature on the rate and extent of growth of Clostridium botulinum type A in pasteurized pork slurry. J. Appl. Bacteriol., 62, 479-490 (1987).   DOI
6 Davey, K. R.: A predictive model for combined temperature and water activity on microbial growth during the growth phase. J. Appl. Bacteriol., 67, 483-488 (1989).   DOI
7 Ratkowsky, D. A., Olley, J., McMeekin, T. A., Ball, A.: Relationship between temperature and growth rate of bacterial cultures. J. Bacteriol., 149, 1-5 (1982).
8 McMeekin, T. A., Olley, J., Ross, T.: Predictive microbiology: theory and application (1993).
9 Ross, T.: Indices for performance evaluation of predictive models in food microbiology. J. Appl. Bacteriol., 81, 501-508 (1996).
10 McDonald, K., Sun, D.W.: Predictive food microbiology for the meat industry: a review. Int. J. Food Microbiol., 52, 1-27 (1999).   DOI
11 Geeraerd, A.H., Valdramidis, V.P., Van Impe, J.F.: GInaFiT, a freeware tool to assess non-log-linear microbial survivor curves. Int. J. Food Microbiol., 102, 95-105 (2005).   DOI
12 Abou-Zeid, K. A., Oscar, T. P., Schwarz, J. G., Hashem, F. M., Whiting, R. C., Yoon, K.: Development and validation of a predictive model for Listeria monocytogenes Scott A as a function of temperature, pH, and commercial mixture of potassium lactate and sodium diacetate. J. Microbiol. Biotechnol., 19, 718-726 (2009).
13 Oscar, T. P.: Development and validation of primary, secondary, and tertiary models for growth of Salmonella Typhimurium on sterile chicken. J. Food Prot., 68, 2606-2613 (2005).   DOI
14 Ratkowsky, D.A.: Model fitting and uncertainty. In: McKellar, R., Lu, X. (Eds.), Modeling Microbial Responses in Foods. CRC Press, Boca Raton, ISBN: 0-8493-1237-X, 151-196 (2003).
15 McQuestin, O. J., Musgrove, M. T., Tamplin, M. L.: Kinetics of growth and inactivation of Salmonella enterica serotype Typhimurium DT104 in pasteurised liquid egg products. J. Food Microbiol., 27, 396-402 (2010).   DOI
16 Jan, S.: Scientific Opinion on the public health risks of table eggs due to deterioration and development of pathogens1. EFSA J., 12, 1-147 (2014).
17 Mukhopadhyay, S., Ukuku, D., Phillips, J. G., Juneja, V. K.: Survival and growth of Salmonella enterica serovar Enteritidis in membrane-processed liquid egg white with pH, temperature, and storage conditions as controlling factors. J. Food Prot., 75, 1219-1226 (2012).   DOI
18 Moreau, M.R., Wijetunge, D.S.S., Bailey, M.L., Gongati, S.R., Goodfield, L.L., Hewage, E.M.K.K., Jayarao, B. M.: Growth in Egg Yolk Enhances Salmonella Enteritidis Colonization and Virulence in a Mouse Model of Human Colitis. PLoS ONE, 11, e0150258 (2016).   DOI
19 Gumudavelli, V., Subbiah, J., Thippareddi, H., Velugoti, P. R., Froning, G.: Dynamic predictive model for growth of Salmonella Enteritidis in egg yolk. J. Food Sci., 72, M254-M262 (2007).   DOI
20 Jo, H.J., Choi, B.G., Wu Yan, Moon, J.S., Kim, Y.J., Yoon, K.S.: Microbiological Quality and growth and survival of foodborne pathogens in ready-to-eat egg products. J. Fd Hyg. Safety, 30, 178-188 (2015).   DOI
21 Threlfall, E.J., Wain. J., Peters. T., Lane. C., De Pinna. E., Little. C.L., Wales. A.D., Davies. R.H.: Egg-borne infections of humans with Salmonella: not only an S. enteritidis problem. Worlds Poult Sci J., 70, 15-26 (2014).   DOI
22 eKAPEpia: The per captia annual consumption of egg. Available from: http://www.ekapepia.com/user/distribution/distDetail.do?nd94541. Accessed on Dec. 29, 2014 (2014).
23 MFDS (Ministry of Food and Drug Safety): Standards for Processing and Ingredients Specifications of Livestock Products. Available from: http://www.lawnb.com/data/Focuslawdata/lawnbfocusB00047515248.pdf. Accessed on Nov. 12, 2015 (2015).
24 Kang, G.H., Cho, S.H., Seong, P.N., Park, B.Y., Ham, J.S., Jeong, S.G., Kim, D.H., Chae, H.S.: Microbial and Physicochemical Properties of Liquid Egg during Cold Storage. Korean J. Food Sci. An., 31, 557-562 (2011).   DOI
25 Yang, S.Y., Hong, Y.H., Lee, H.J., Song, C.S.: Hygienic management for Salmonella-free chicken Meat. Korean J. Poult. Sci., 37, 289-295 (2010).   DOI
26 Chun, M.S., Hong, S.H.: Identification of Microorganisms from Eggs in Hypermarket in the Northern Gyeonggi Area. Korean J. Food & Nutr., 22, 396-401(2009).
27 Harriet, W., Kirstin, R.: Salmonella and eggs: From Production to Plate. Int. J. Environ. Res. Public Health., 12, 2543-2556 (2015).   DOI
28 Helwigh, B., Korsgaard, H.: The community summary report on trends and sources of zoonoses, zoonotic agents, antimicrobial resistance and foodborne outbreaks in the European Union in 2006. EFSA J. 223, 312 (2007).
29 Gantois, I., Ducatelle, R., Pasmans, F., Haesebrouck, F., Gast, R., Humphrey, T.J., Van Immerseel, F.: Mechanisms of egg contamination by Salmonella Enteritidis. FEMS Microbiol. Rev, 33, 718-738 (2009).   DOI
30 FSIS (Food Safety and Inspection Serviece).: Risk Assessments of Salmonella Enteritidis in Shell Eggs and Salmonella spp. in Egg Products. Available from: www.fsis.usda.gov/shared/PDF/SE_Risk_Assess_Oct2005.pdf. Accessed on Oct. 1, 2005 (2005).
31 Singh, A., Korasapati, N.R., Juneja, V.K., Subbiah, J., Froning, G., Thippareddi, H.: Dynamic predictive Model for the Growth of Salmonella spp. in Liquid Whole Egg. J. Food Sci., 76, 225-232 (2011).
32 MFAFF (Ministry for Food, Agriculture, Forestry and Fisheries): Comprehensive countermeasures of hygiene management of egg product. Available from: https://www.poultry.or.kr:456/_rb_m/_view.html?Ncode=notice&number=35673 &page=. Accessed on Mar. 18, 2010 (2010).
33 Ministry of Food and Drug Safety:Statistical system of food poisoning.(2015). Available from https://www.foodsafetykorea.go.kr/portal/healthyfoodlife/foodPoisoningStat.do?menu_no=519&menu_grp=MENU_GRP02.