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http://dx.doi.org/10.13103/JFHS.2016.31.5.319

Development of Analytical Method for Colistin in Fish and Shrimp using Liquid Chromatography Mass Spectrometry  

Shin, Dasom (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Kang, Hui-Seung (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Lee, Soo-Bin (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Cho, Yoon-Jae (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Cheon, So-Young (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Jeong, Jiyoon (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Rhee, Gyu-Seek (Pesticide and Veterinary Drug Residues Division, National Institute of Food & Drug Safety Evaluation)
Publication Information
Journal of Food Hygiene and Safety / v.31, no.5, 2016 , pp. 319-326 More about this Journal
Abstract
Colistin is a last resort antimicrobial agent against multi-drug resistant Gram-negative bacteria. This study was conducted to develop an analytical method to determine colistin in fish and shrimp. The analytes were confirmed and quantified via liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the positive ion mode using multiple reaction monitoring (MRM). The sample was extracted with acidified 5% methanol (containing 0.5% formic acid). Then, solid phase extraction (SPE) was used for cleanup. Matrix-matched calibration curves were linear over the calibration ranges (0.05-1.2 mg/kg) for all the analytes into blank sample with $r^2$ > 0.99. All the values fulfilled the criteria requested by the Codex guidelines. Average recoveries ranged from 85.9% to 107.9%. The repeatability of measurements, expressed as the coefficient of variation (CV, %), was less than 15%. The limit of detection (LOD) was 0.02 mg/kg, and the limit of quantitation (LOQ) was 0.05 mg/kg. This improved method showed higher accuracy and acceptable sensitivity to meet the CAC guideline requirements and is applicable for the analysis of residual colistin (A+B) in fish and shrimp.
Keywords
colistin; fish; shrimp; analytical method; LC-MS/MS;
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Times Cited By KSCI : 1  (Citation Analysis)
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