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http://dx.doi.org/10.13103/JFHS.2013.28.1.041

Apoptotic Activity of Methanol Extract of Tongcao in HEp-2 Human Cervical Cancer Cells

Choi, Eun-Sun (Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience, Brain Korea 21, Chonbuk National University)
Jung, Ji-Youn (Department of Companion and Laboratory Animal Science, Kongju National University)
Lee, Hang-Eun (Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience, Brain Korea 21, Chonbuk National University)
Cho, Sung-Dae (Department of Oral Pathology, School of Dentistry, Institute of Oral Bioscience, Brain Korea 21, Chonbuk National University)

Publication Information

Journal of Food Hygiene and Safety / v.28, no.1, 2013 , pp. 41-44 More about this Journal

Abstract

Although tongcao has been reported to have anti-oxidative, anti-inflammatory and antipyretic effects, there is no report of the chemopreventive effect of tongcao in cancer cells. In the present study, we investigated the anti-proliferative activity of methanol extract of tongcao (MET) and its molecular target in HEp-2 human cervical cancer cells using MTS assay, western blot analysis, and DAPI staining. MET significantly decreases cell viability and induces apoptotic cell death. It affects Bid protein to be truncated resulting in the release of cytochrome c from mitochondria to cytosol whereas it did not affect other Bcl-2 family members. Thus, we clearly suggest that tongcao can be a potential naturally occurring plants having chemopreventive activity in cervical cancer.

Keywords

Tongcao; Chemoprevention; Apoptosis; Cervical cancer;

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