[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.13103/JFHS.2012.27.4.332

Development and Validation of Analytical Methods for Picoxystrobin Determination in Agricultural Products by GC-ECD and GC-MS

Do, Jung-Ah (Food Chemical Residues Division, National Institute of Food and Drug Safety Evaluation, KFDA)
Lee, Min-Hye (Food Chemical Residues Division, National Institute of Food and Drug Safety Evaluation, KFDA)
Park, Hyejin (Food Chemical Residues Division, National Institute of Food and Drug Safety Evaluation, KFDA)
Kang, Il-Hyun (Food Chemical Residues Division, National Institute of Food and Drug Safety Evaluation, KFDA)
Kwon, Kisung (Food Chemical Residues Division, National Institute of Food and Drug Safety Evaluation, KFDA)
Oh, Jae-Ho (Food Chemical Residues Division, National Institute of Food and Drug Safety Evaluation, KFDA)

Publication Information

Journal of Food Hygiene and Safety / v.27, no.4, 2012 , pp. 332-338 More about this Journal

Abstract

A simple and sensitive analytical method was developed using gas chromatography with electron capture detector (GC-ECD) and gas chromatography-mass spectrometry (GC-MS) for determination of Picoxystrobin in agricultural products (apple, hulled rice, mushroom, pepper, soybean, and mandarin). Picoxystrobin residues were extracted with acetonitrile, partitioned with saline water, and then they were cleaned up on a florisil solid-phase extraction (SPE) cartridge to obtain an extract suitable for analysis by GC-ECD and GC-MS. The method was validated using 6 agricultural product samples spiked with Picoxystrobin at different concentration levels (0.02, 0.05 and 0.5 mg/L). Average recoveries of Picoxystrobin (using each concentration three replicates) ranged 64.0~98.3% with relative standard deviations less than 10%, calibration solutions concentration in the range 0.1~5 mg/L, and limit of detection (LOD) and limit of quantification (LOQ) were 0.005 and 0.02 mg/L, respectively. The result showed that the developed analytical method is suitable for Picoxystrobin determination in agricultural products.

Keywords

Picoxystrobin; acetonitrile extraction; GC-ECD; GC-MS;

Citations & Related Records

Reference

1	Barlett, D.W., Clough, J.M., J.R. Godwin, J.R., Hall, A.A., Hamer, M., and Parr-Dobrzanski, B.: The strobilurin fungicides (review). Pest. Manag. Sci. 58, 649-662 (2002). DOI ScienceOn
2	Genay, J.P., Morand, P., and Dieleman, P.: Picoxystrobin: a fungicide for use on cereals.Phytoma. La Defense des Vegetaux 555, 56-58 (2002).
3	Syngenta Crop (UK): Acanto Product Use, Product Guide. Available at www.syngenta-crop.co.uk (2005).
4	Godwin, J.R., Bartlett, D.W., Clough, J.M., Godfrey, C.R.A., Harrison, E.G., and Maund, S.: The BCPC Conference: Pests and diseases, Volume 2. Proceedings of an international conference held at the Brighton Hilton Metropole Hotel, Brighton, UK pp. 533-540 (2000).
5	Bartlett, D.W., Clough, J.M., Godfrey, C.R.A., Godwin, J.R., Hall, A.A., Heaney, S.P., and Maund, S.J.: Understanding the strobilurin. R. Soc. Chem. (2001).
6	European commission: Commission Directive 2003/84/EC of 25 September 2003 amending Council Directive 91/414/EEC to include flurtamone, flufenacet, iodosulfuron, dimethenamid- P, Picoxystrobin, fosthiazate, and silthiofam as active substances, Off. J. Eur. Union L-247 20-25 (2003)
7	US Environmental Protection Agency: Pesticides: Regulating Pesticides, Multi-Year Work plan for Registration of Conventional Pesticides - New Chemicals, EPA, USA. Available at http://www.epa.gov/opprd001/workplan/newchem.html (2012).
8	Vinas, P., Campillo, N., Martinez-Castillo, N., Hernandez-cordoba, M.: Method development and validation for strobilurin fungicides in baby foods by solid-phase microextraction gas chromatography-mass spectrometry. J. Chromatogr. A 1216, 140-146 (2009). DOI
9	Rural Development Administration: RDA Notice 2012-15 (2012).
10	Korea Crop Protection Association: Pesticide Use Guideline (2011).
11	Korean Food and Drug Administration: KFDA Notice 2011- 23 (2011).
12	Korean Food and Drug Administration: KFDA Notice 2012-100 (2012).
13	Ho, W., and Hsieh, S.J.: Solid phase micro extraction associated with microwave assisted extraction of organ chlorine pesticides in medicinal plants. Anal. Chim. Acta. 428, 111 (2001). DOI ScienceOn
14	Zuin, V.G., Yariwake, J., and Langas, .M.: Analysis of esicide residues in Brazilian plans. Braz. Hem. Soc. 14, 304-09 (2003). DOI ScienceOn
15	Rao, T.N., Ramesh, A., Parnathamma, P., and Suresh, G.: Development and validation of a HPLC-UV method for simultaneous determination of strobilurin fungicde esidues m tomato fruits followed by matrixs solid-phase dispersion (MSPD). Indian. Sci Res. 113-118 (2012).
16	Gonzalez-Rodriguez, R.M., Cancho-Grande, B., and Simal- Gandara, J.:Multi-residue determination of 11 new fungicides in grapes and wines by liquid-liquid extraction/cleanup and programmable temperature vaporization injection with analyte protectants/gas chromatography/ion trap mass spectrometry. J. Chromatogr. A 1216, 6033-6042 (2009). DOI
17	Tekel, J. and Hatrik, S.: Pesticide residue analyses in plant material by chromatographic methods-clean-up procedures and selective detectors. J. Chromatogr. A 754, 397-410 (1996). DOI
18	Topuz, S. Ozhan, G., and Alpertunga, B.: Simultaneous determination of various pesticides in fruit juices by HPLCDAD. Food Control 16, 87-92 (2005). DOI ScienceOn
19	Mou, R.X., Chen, M.X., and Zhi, J.L.: Simultaneous determination of 15 phenylurea herbicides in rice and corn using HPLC with fluorescence detection combined with UV decomposition and post-column derivatization. J. Chromatogr. B 875, 437-443 (2008). DOI ScienceOn
20	Korean Food and Drug Administration: Korean Food Standards Codex (2011).