Browse > Article

Protective Effect of Selenium on Experimental Colon Carcinogenesis in Mice Fed a Low Iron Diet  

Park, Hyun-Ji (Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
Kim, Jun-Hyeong (Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
Kang, Bong-Su (Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
Nam, Sang-Yoon (Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
Kim, Jong-Soo (Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
Jeong, Jae-Hwang (Department of Biotechnology and Biomedicine, Chungbuk Province College)
Kim, Eun-Young (Department of Hotel Food Service & Culinary Arts, Youngdong University)
Lee, Beom-Jun (Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
Yun, Young-Won (Department of Veterinary Medicine, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
Publication Information
Journal of Food Hygiene and Safety / v.26, no.4, 2011 , pp. 388-397 More about this Journal
Abstract
Selenium (Se) is known to prevent from several cancers, while iron (Fe) is known to be associated with high risk of cancers. The role of Se on colon carcinogenesis was investigated in an animal model induced by azoxymethane (AOM) and dextran sodium sulfate (DSS) in low Fe mice. Six-week old ICR mice fed on a low Fe diet (4.5 ppm Fe; generally 10 times lower than normal Fe) with three different Se (0.02, 0.1 or 0.5 ppm) levels for 24 weeks. The animals received weekly three ($0{\sim}2^{nd}$ weeks) i.p. injections of AOM (10 mg/kg RW), followed by 2% DSS with drinking water for 1 week to induce the colon cancer. There were five experimental groups including vehicle, positive control (normal Fe level, AOM/DSS), Low Fe (LFe) + AOM/DSS+Low Se (LSe), LFe + AOM/DSS + medium Se (MSe) and LFe + AOM/DSS + high Se (HSe) groups. HSe group showed a 66.7% colonic tumor incidence, MSe group showed a 69.2% tumor incidence, and LSe group showed a 80.0% tumor incidence. The tumor incidence was negatively associated with Se levels of diets. Tumor multiplicity in Hse group was significantly low compared to the other groups (p < 0.05). With increasing Se levels of diets, the primary anti-proliferating cell nuclear antigen (PCNA)-positive cells were decreased and apoptotic bodies were increased in a dose-dependent manner. Se-dependent glutathione peroxidase activity and its protein level were dependent on the levels of Se of diets. Malondialdehyde level in liver was lowest in Hse group among experimental groups. These findings indicate that dietary Se is chemopreventive for colon cancer by increasing antioxidant activity and decreasing cell proliferation in Fe-deficient mice.
Keywords
Selenium; Iron; Colon cancer; Azoxymethane; Dextran sodium sulfate;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Connelly-Frost, A., Poole, C., Satia, J.A., Kupper, L.L., Millikan, R.C. and Sandler, R.S.: Selenium, apoptosis, and colorectal adenomas. Cancer Epidemiol. Biomarkers Prev. 15, 486-493 (2006).   DOI   ScienceOn
2 Combs, G.F. Jr., Clark, L.C. and Turnbull, B.W.: An analysis of cancer prevention by selenium. Biofactors, 14,153-159 (2001).   DOI
3 Evans, P. and Halliwell, B.: Micronutrients: oxidant/antioxidant status. Br. J. Nutr., 85 (Suppl. 2), S67-74 (2001).
4 Beckman, K.B. and Ames, B.N.: The free radical theory of aging matures. Physio.l Rev., 78, 547-581 (1998).
5 Larsson, S.C. and Wolk, A.: Meat consumption and risk of colorectal cancer: a meta-analysis of prospective studies. Int. J. Cancer, 119, 2657-2664 (2006).   DOI   ScienceOn
6 Norat, T. and Riboli, E.: Meat consumption and colorectal cancer: a review of epidemiologic evidence. Nutr. Rev., 59, 37-47 (2001).
7 Jemal, A., Siegel, R., Ward, E., Hao, Y., Xu, J. and Thun, M.J.: Cancer statistics. Ca-Cancer J. Clin., 2009, 59, 225-249 (2009).   DOI   ScienceOn
8 Okayasu, I., Hatakeyama, S., Yamada, M., Ohkusa, .T, Inagaki, Y. and Nakaya, R.: A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology, 98, 694-702 (1990).
9 Rosenberg, D.W., Giardina, C. and Tanaka, T.: Mouse models for the study of colon carcinogenesis. Carcinogenesis, 30, 183-196 (2009).
10 Bruce, W.R., Giacca, A. and Medline, A.: Possible mechanisms relating diet and risk of colon cancer. Cancer Epidemiol. Biomarkers Prev., 9, 1271-1279 (2000).
11 Rudolf, E., Kralova, V. and Cervinka, M.: Selenium and colon cancer--from chemoprevention to new treatment modality. Anti-Cancer Agents Med. Chem., 8, 598-602 (2008).
12 Feng, Y., Finley, J.W., Davis, C.D., Becker, W.K., Fretland, A.J. and Hein, D.W.: Dietary selenium reduces the formation of aberrant crypts in rats administered 3,2'-dimethyl-4-aminobiphenyl. Toxicol. Appl. Pharmacol., 157, 36-42 (1999).   DOI   ScienceOn
13 Finley, J.W. and Davis, C.D.: Selenium (Se) from high-selenium broccoli is utilized differently than selenite, selenate and selenomethionine, but is more effective in inhibiting colon carcinogenesis. Biofactors, 14, 191-196 (2001).   DOI
14 Combs, G.F. Jr. and Combs, S.B.: The nutritional biochemistry of selenium. Annu. Rev. Nutr., 4, 257-280 (1984).   DOI   ScienceOn
15 Patrick, L.: Selenium biochemistry and cancer: a review of the literature. Altern. Med. Rev., 9, 239-258 (2004).
16 Schrauzer, G.N.: Anticarcinogenic effects of selenium. Cell Mol. Life Sci., 57, 1864-1873 (2000).   DOI   ScienceOn
17 Butler, J.A., Thomson, C.D., Whanger, P.D. and Robinson, M.F.: Selenium distribution in blood fractions of New Zealand women taking organic or inorganic selenium. Am. J. Clin. Nutr., 53, 748-754 (1991).
18 Stevens, R.G. and Kalkwarf, D.R.: Iron, radiation, and cancer. Environ Health Perspect., 87, 291-300 (1990).
19 Ravn-Haren, G., Bugel, S., Krath, B.N., Hoac, T., Stagsted, J., Jorgensen, K., Bresson, J.R., Larsen, E.H. and Dragsted, L.O.: A short-term intervention trial with selenate, seleniumenriched yeast and selenium-enriched milk: effects on oxidative defence regulation. Br. J. Nutr., 99, 883-892 (2008).
20 Tanaka, T., Kohno, H., Suzuki, R., Yamada, Y., Sugie, S. and Mori H.: A novel inflammation-related mouse colon carcinogenesis model induced by azoxymethane and dextran sodium sulfate. Cancer Sci., 94, 965-973 (2003).   DOI   ScienceOn
21 Murawaki, Y., Tsuchiya, H., Kanbe, T., Harada, K., Yashima, K., Nozaka, K., Tanida, O., Kohno, M., Mukoyam,a T., Nishimuki, E., Kojo, H., Matsura, T., Takahashi, K., Osaki, M., Ito, H., Yodoi, J. and Shiota, G.: Aberrant expression of selenoproteins in the progression of colorectal cancer. Cancer Lett., 259, 218-230 (2008).   DOI   ScienceOn
22 Ashokkumar, P. and Sudhandiran, G.: Protective role of luteolin on the status of lipid peroxidation and antioxidant defense against azoxymethane-induced experimental colon carcinogenesis. Biomed. Pharmacother, 62, 590-597 (2008).   DOI   ScienceOn
23 Beno, I., Klvanova, J., Magalova, T. and Brtkova, A.: Blood levels of natural antioxidants in gastric and colorectal precancerous lesions and cancers in Slovakia. Neoplasma, 47, 37-40 (2000).
24 Tanaka, T., de Azevedo, M.B., Duran, N., Alderete, J.B., Epifano, F., Genovese, S., Tanaka, M. and Curini, M.: Colorectal cancer chemoprevention by 2 beta-cyclodextrin inclusion compounds of auraptene and 4'-geranyloxyferulic acid. Int. J. Cancer, 126, 830-840 (2010).
25 Youn, B.W., Fiala, E.S. and Sohn, O.S.: Mechanisms of organoselenium compounds in chemoprevention: effects on transcription factor-DNA binding. Nutr. Cancer, 40, 28-33 (2001).   DOI   ScienceOn
26 Ilsley, J.N., Belinsky, G.S., Guda, K., Zhang, Q., Huang, X., Blumberg, J.B., Milbury, P.E., Roberts, L.J., 2nd, Stevens, R.G. and Rosenberg, D.W.: Dietary iron promotes azoxymethane-induced colon tumors in mice. Nutr. Cancer, 49, 162-169 (2004).   DOI   ScienceOn
27 Lund, E.K., Wharf, S.G., Fairweather-Tait, S.J. and Johnson, I.T.: Increases in the concentrations of available iron in response to dietary iron supplementation are associated with changes in crypt cell proliferation in rat large intestine. J. Nutr., 128, 175-179 (1998).
28 Soyars, K.E. and Fischer, J.G.: Iron supplementation does not affect cell proliferation or aberrant crypt foci development in the colon of sprague-dawley rats. J. Nutr., 128, 764-770 (1998).
29 Clark, L.C., Combs, G.F., Jr., Turnbull, B.W., Slat,e E.H., Chalker, D.K., Chow, J., Davis, L.S., Glover, R.A., Graham, G.F., Gross, E.G., Krongrad, A., Lesher, J.L., Jr., Park, H.K., Sanders, B.B., Jr., Smith, C.L. and Taylor, J.R.: Effects of selenium supplementation for cancer prevention in patients with carcinoma of the skin. A randomized controlled trial. Nutritional Prevention of Cancer Study Group. J. Am. Med. Assoc., 276, 1957-1963 (1996).   DOI   ScienceOn
30 Britton, R.S.: Metal-induced hepatotoxicity. Semin. Liver Dis, 16, 3-12 (1996).   DOI   ScienceOn