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Role of p53-dependent PI3K in Radioresistance of Colon Cancer Cells  

Lee, Heui-Kwan (Department of Radiation Oncology, Presbyterian Medical Center)
Kim, Jong-Suk (Department of Biochemistry, Chonbuk National University Medical School)
Kwon, Hyoung-Cheol (Department of Radiation Oncology & Research Institute of Clinical Medicine, Chonbuk National University Hospital)
Publication Information
Journal of Food Hygiene and Safety / v.25, no.3, 2010 , pp. 258-262 More about this Journal
Abstract
Radiotherapy is one of the major therapies for cancer treatment. p53 acts as a central mediator of the cellular response to stressful stimuli, such as radiation. Recently it has been known that activation of the phosphatidylinositol-3-kinase (PI3K) pathway is associated with radioresistance. In this study, we investigated whether X-irradiation up-regulates PI3K in a p53-dependent manner in human colon cancer cells. In order to study this phenomenon, we have treated p53-wild type and p53-mutant type HCT116 cells with X-ray. Treatment of wild type HCT116 cells with 8 Gy resulted in a marked increase in PI3K (p85), which paralleled an increase in PTEN, a counterpart of PI3K. However, these effects of X-rays in the p53-mutant cells were not observed. These results suggest that the X-irradiation-induced up-regulation of PI3K/PTEN pathway is p53-dependent.
Keywords
X-irradiation; p53; PI3K; PTEN; Colon cancer;
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