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Comparison of Macroscopic Inspection and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) for the Detection of Anisakis simplex complex  

Kang, Ju-Hee (Department of Food and Nutrition, College of Human Ecology, Chung-Ang University)
Lee, Min-Hwa (Department of Food and Nutrition, College of Human Ecology, Chung-Ang University)
Lee, Kang-Bum (Department of Food and Nutrition, College of Human Ecology, Chung-Ang University)
Choi, Chang-Sun (Department of Food and Nutrition, College of Human Ecology, Chung-Ang University)
Publication Information
Journal of Food Hygiene and Safety / v.23, no.4, 2008 , pp. 314-318 More about this Journal
Abstract
This research aimed to compare the detection methods of Anisakis simplex in Sea fish by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and macroscopic inspection. We examined 18 Trichiurus lepturus, 11 Scomber japonicus, and 65 Todarodes pacificus collected from the retail markets in the areas of Uljin, Kyuonggi province and Seoul. As the result of examinations, we found that detection rate of Anisakis simplex by macroscopic observation was 89% in Trichiurus lepturus, 90.9% in Scomber japonicus, 32.3% in Todarodes pacificus. The detection rate of Anisakis simplex by PCR-RFLP was 77.7% in Trichiurus lepturus, 81.8% in Scomber japonicus, 26.1% in Todarodes pacificus. We could conclude that PCR-RFLP method of Anisakis simplex was more specific rather than macroscopic observation.
Keywords
Anisakis simplex complex; Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP); Trichiurus lepturus; Scomber japonicus; Todarodes pacificus;
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1 Van Thiel, P. H., Kuipers, F. C., Raskam, R. T.: A nematode parasitic to herring, causing acute abdominal syndroms in man. Trophic Geographic Medicine, 2, 97-113, (1960)
2 Yokogawa, M., Yoshimura, H.: Clinicopathologic studies on larval anisakiasis in Japan. Am. J. Trop. Med. Hyg. 16, 723- 728 (1967)   DOI
3 Cho, S. Y., Chi, J. G., Kim, I. S.: A case human anisakiasis in Korea. Seoul J Med. 21, 203-208 (1980)
4 Lee, A. H., Kim, S. K., Choi, K. Y.: A case of human infection with the larva of Terranova type A. Korean J. Parasitol. 19, 463-467 (1985)
5 Sohn, W. M., Seoul, S. Y.: A human case of gastric anisakiasis by Pseudoterranova decipiens larva. Korean J. Parasitol. 32, 53-56 (1994)   DOI
6 D'Amelio, S., Mathiopoulos, K.D., Brandonisio, O., Lucarelli, G., Doronzo, F., Paggi, L. Diagnosis of a case of gastric anisakidosis by PCR-based restriction fragment length polymorphism analysis. Parassitologia, 41, 591-593 (1999)
7 Umehara, A., Kwakami, Y., Matsui, T., Araki, J., Uchida, A. Molecular identification of Anisakis simplex sensu stricto and Anisakis pegreffii (nematoda: Anisakidae) from fish and cetacean in Japanese waters. Parasitology Int. 55, 267-271 (2006)   DOI   ScienceOn
8 Woo, H. C., Kim, J. A.: The Infection Status and Identification of Anisakid Larvae in Marine Fish Caught from the sea Near Cheju Island, Kor. J. Vet. Publ. Hlth 24, 690-696 (2000)
9 Zhu, X., Gasser, R. B., Podolska, M, Chilton, N. B. Characterisation of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. Int. J. Parasitol. 28. 1911-1921 (1998)   DOI   ScienceOn
10 Umehara, A., Kawakami, Y., Araki, J., Uchida, A. Molecular identification of the etiological agent of the human anisakiasis in Japan. Parasitology Int. 56, 211-215 (2007)   DOI   ScienceOn
11 Sakanari, J. A., McKerrow, J. H. Anisakiasis. Clin. Microbiol. Rev. 2, 278-284 (1989)   DOI
12 Szostakowska, B., Myjak, P., Kur, J. Identification of aniskid nematodes from the Southern Baltic Sea using PCR-based methods. Mol. Cell. Probes. 16, 111-118 (2002)   DOI   ScienceOn
13 Im, K. I., Shin, H. J., Kim, B. H., Moon, S. I.: Gastric anisakiasis cases in cheju-do, Korea. Korean J. Parasitol. 33, 179-186 (1995)   DOI   ScienceOn
14 Kim, C. H., Chung, B. S., Moon, Y. I., Chun, S. H. : A case report on human infection with Anisakis sp. in Korea. Korean J. Parasitol., 9, 39 (1971)   DOI