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Effect of Hot Water and Microwave Heating on the Inactivation of Enterobacter sakazakii in Reconstituted Powdered Infant formula and Sunsik  

Kim, Jung-Beom (Health Research Department, Gyeonggi-do Institute of Health & Environment)
Park, Yong-Bae (Health Research Department, Gyeonggi-do Institute of Health & Environment)
Lee, Myung-Jin (Health Research Department, Gyeonggi-do Institute of Health & Environment)
Kim, Ki-Cheol (Health Research Department, Gyeonggi-do Institute of Health & Environment)
Huh, Jeong-Weon (Health Research Department, Gyeonggi-do Institute of Health & Environment)
Kim, Dae-Hwan (Health Research Department, Gyeonggi-do Institute of Health & Environment)
Lee, Jong-Bok (Health Research Department, Gyeonggi-do Institute of Health & Environment)
Kim, Jong-Chan (Health Research Department, Gyeonggi-do Institute of Health & Environment)
Choi, Jae-Ho (Division of Food and Biotechnology, Kangwon National University)
Oh, Deog-Hwan (Division of Food and Biotechnology, Kangwon National University)
Publication Information
Journal of Food Hygiene and Safety / v.23, no.2, 2008 , pp. 157-162 More about this Journal
Abstract
Enterobacter sakazakii was initially referred to as yellow-pigmented Enterobacter cloacae and reclassified in 1980. E. sakazakii infection cause life-threatening meningitis, septicemia, and necrotizing enterocolitis in infants. Powdered infant formula (PIF) and baby foods may be the important vehicle of E. sakazakii infection. It has been reported that E. sakazakii was isolated from PIF and sunsik ingredients produced in Korea. Some infants have been fed sunsik as a weaning diet. Therefore, it is necessary that this organism should be inactivated on preparing PIF and sunsik at homes and in hospitals. The cocktail of three Korean E. sakazakii strains (human, sunsik and soil isolates) were used to investigate the inactivation of this organism with hot water at 50, 60, 65, 70 and $80^{\circ}C$ and microwave heating for 60, 75, 90, 105 and 120 sec. Reconstituted PIF and sunsikwere inoculated with cocktailed vegetative cells of E. sakazakii at 6 log CFU/mL. Thermal inactivation of vegetative cells of E. sakazakii were achieved by reconstituted PIF and sunsik with hot water at $60^{\circ}C$ or greater and with microwave heating at 2,450 MHz for 75 sec or longer. Considering that biofilm formation of E. sakazakii was adapted to survive the dry environment that is PIF and sunsik and thermal resistance increased, it is suggested that inactivation of E. sakazakii was used by hot water at $70^{\circ}C$ or greater and microwave heating for 90 sec or longer. Reconstituted PIF and sunsik were inoculated with cocktailed vegetative cells of E. sakazakii at 2 to 3 log CFU/mL to investigate the growth curve of this organism and stored at 5, 10, 15, 20, 25, 30 and $35^{\circ}C$. Viable counts slightly changed at 5, $10^{\circ}C$ during 48 h but grew at $15^{\circ}C$ or greater. Considering that E. sakazakii is able to grow in infant formula milk at refrigerator temperature, reconstituted PIF and sunsik that are not immediately consumed should be discarded or stored at refrigeration temperatures within 24 h.
Keywords
Enterobacter sakazakii; Inactivation; Hot water; Microwave heating;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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1 Iversen, C., Druggan, P., and Forsythe, S. : A selective differential medium for Enterobacter sakazakii, a preliminary study. Int. J. Food Microbiol., 96, 133-139 (2004)   DOI   ScienceOn
2 Biering, G., Karlsson, S., Clark, N.V.C., Jonsdottir, K.E., Ludvigsson, P., and Steingrimsson, O. : Three cases of neonatal meningitis caused by Enterobacter sakazakii in powdered milk. J. Clin. Microbiol., 27, 2054-2056 (1989)
3 Choi, J.W., Kim, Y.J., Lee, J.K., Kim, Y.H., Kwon, K.S., Hwang, I.G., and Oh, S.W. : Multiple confirmation and RAPD-genotyping of Enyerobacter sakazakii isolation from Sunsik. Korean J. Food Sci. Technol., 40(1), 101-105 (2008)   과학기술학회마을
4 Doyle, M.P. and Roman, D.J. : Growth and survival of Campylobacter fetus subsp. jejuni as a function of temperature and pH. J. Food Prot., 44, 596-601 (1981)   DOI
5 Edelson-Mammel, S.G. and Buchanan, R.L. : Thermal inactivation of Enterobacter sakazakii in rehydrated infant formular. J. Food Prot., 67, 60-63 (2004)   DOI
6 Goldblith, S.A., Wang, D.I.C. : Effect of microwaves on Escherichia coli and Bacillus subtilis. Appl. Microbiol., 15, 1371-1375 (1967)
7 Nazarowec-White, M. and Farber, J.M. : Thermal resistance of Enterobacter sakazakii in reconstituted dried-infant formular. Lett. Appl. Microbiol., 24, 9-13 (1997)   DOI   ScienceOn
8 Durdy, D., Mullane, N.R., Quinn, T., Wall, P.G., and Fanning, S. : Enterobacter sakazakii: An emerging pathogen in powdered infant formula. Clin. Infect. Dis., 42, 996-1002 (2006)   DOI   ScienceOn
9 Pagotto, F.J., Nazarowec-White, M., Bidawid, S., and Farber, J.M. : Enterobacter sakazakii: infectivity and enterotoxin production in vitro and in vivo. J. Food Prot., 66, 370-375 (2003)   DOI
10 Burkhard, M. and Martin, W. : Detection of Enterobacter sakazakii strains by real-time PCR. J. Food Prot., 68(8), 1623-1627 (2005)   DOI
11 Kim,S., Yi, H.C., Kim, E.M., Lee, M.A., Park, J.A., and Kim, J.W. : Assessment of microbial contamination levels of elementary school classrooms as foodservice environments. Korean J. Food Cookery Sci., 23(3), 321-326 (2007)   과학기술학회마을
12 Iversen, C., Lane, M., and Forsythe, S. : The growth profile, thermotolerance and biofilm formation of Enterobacter sakazakii growth in infant formula milk. Lett. Appl. Microbiol., 38, 378-382 (2004)   DOI   ScienceOn
13 Yoo, M.K., Kim, S.S., and Oh, S. : Isolation and genotyping of Enterobacter sakazakii from powdered infant formula manufactured in Korea. Food Sci. Biotechnol., 16(6), 875-877 (2005)
14 Farmer, J.J., Asbury, M.A., Hickman, F.W., and Don, J.B. : Enterobacter sakazakii: A new species of "Enterobacteriaceae" isolation from clinical specimens. Int. J. Syst. Bacteriol., 569-584 (1980)
15 Barbara, J.S., Nellie, H., Avroy, A.F., and James, A.L.: Enterobacter sakazakii is a rare cause of neonatal septicemia or meninggitis in VLBW infants. J. Pediatrics, 821-823 (2004)
16 Centers for Disease Control and Prevention : Enterobacter sakazakii infections associated with the use of powdered infant formula-Tennessee 2001. Morb. Mortal. Wkly. Rep., 51, 297-300 (2002)
17 Gurtler, J.B., Kornacki, J.L., and Beuchat, L.R. : Enterobacter sakazakii, a coliform of increased concern to infant health. Int. J. Food Microbiol., 104, 1-34 (2005)   DOI   ScienceOn
18 Nazarowec-white, M. and Farber, J.M. : Phenotypic and genotypic typing of food and clinical isolates of Enterobacter sakazakii. J. Med. Microbiol., 48, 559-567 (1999)   DOI   ScienceOn
19 Kindle, G., Busse, A., Kampa, D., Meyer-Koenig, U., and Daschner, F.D. : Killing activity of microwaves in milk. J. Hosp. Infect., 33, 273-278 (1996)   DOI   ScienceOn
20 Bardshaw, J.G., Peeler, J.T., Corwin, J.J., Barnett, J.E., and Twedt, R. M. : Thermal resistance of discase-associated Salmonella typhimurium in milk. J. Food Prot., 50, 95-96 (1987)   DOI
21 Iversen, C., Hargreaves, A., and Forsythe S. : Growth rates and D-values of Enterobacter sakazakii in 5 suspending media. ASM conference, Washington, 17-22 May 2003
22 Delphine, G., Laurent, P., Amornrut, L., Amal, K., Chanwitt, T., Michael, F. and Patrice, N. : Molecular epidemiology of the integron-located VEB-1 extended-spectrum $\beta$-lactamase in nosocomial enterobacterial isolates in BangKok, Thailand. J. Clin. Microbiol., 175-182 (2001)
23 Jung, C.W., Kim, O.J., and Min, H.S. : A study of effect of the evaluative accreditation system of daycare center. J. Child Edu., 17, 269-279 (2008)
24 Nazarowec-White, M. and Farber, J.M. : Enterobacter sakazakii: a review. Int. J. Food Microbiol., 34, 103-113 (1997)   DOI   ScienceOn
25 Iversen, C. and Forsythe, S. : Isolation of Enterobacter sakazakii and other enterobacteriaceae from powdered infant formular milk and related products. J. Food Microbiol., 21, 771-777 (2004)   DOI   ScienceOn
26 Knabel, S.J., Walker, H.W., Hartman, P.A., and Mendonea, A.F. : Effect of growth temperature and strictly anaerobic recovery on survival of Listeria monocytogenes during pasteurization. Appl. Environ. Microbiol., 56, 370-376 (1990)
27 Kim, S.H., and Park, J.H. : Thermal resistance and inactivation of Enterobacter sakazakii isolation during rehydration of powdered infant formular. J. Microbiol. Biotechnol., 17(2), 364-368 (2007)   과학기술학회마을