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The Estrogenic Effects of Phthalates (DEHP, DBP) in MCF-7 Cell  

Lee, Su-Youn (Department of Companion and Laboratory Animal Science, Kongju National University)
Kim, So-Jung (Department of Companion and Laboratory Animal Science, Kongju National University)
Lee, Seung-Ho (Department of Companion and Laboratory Animal Science, Kongju National University)
Park, Young-Seok (Department of Companion and Laboratory Animal Science, Kongju National University)
Park, Byung-Kwon (Department of Companion and Laboratory Animal Science, Kongju National University)
Kim, Byeong-Soo (Department of Companion and Laboratory Animal Science, Kongju National University)
Kim, Sang-Ki (Department of Companion and Laboratory Animal Science, Kongju National University)
Choi, Chang-Sun (Department of Food and Nutrition, College of Human Ecology, Chung-Ang University)
Yoon, Seong-Il (Department of Life Science, College of Natural Sciences, Hanyang University)
Kim, Jong-Suk (Department of Biochemistry, Institute of Medical Science, Chunbuk National University Medical School)
Jung, Ji-Won (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Jung, Ji-Youn (Department of Companion and Laboratory Animal Science, Kongju National University)
Publication Information
Journal of Food Hygiene and Safety / v.22, no.3, 2007 , pp. 209-212 More about this Journal
Abstract
To evaluate the estrogenic activities of di-ethyl hexyl phthalate (DEHP) and di-butyl phthalate (DBP), two phthalates known as endocrine disrupters, we used MCF-7 human breast cancer cell line. As results, DBP and DEHP had estrogenic effects. In brief, the concentration of maximal MCF-7 cell proliferation was $10^{-7}M\;and\;10^{-8}M$ for DEHP and DBP, respectively. The ratio of maximal cell yield of the test compounds to that of $17{\beta}-estradiol$ was 87.5% for DEHP and 73.4% for DBP. In summary, both DEHP and DBP had cell proliferation potencies in the MCF-7 cell. Potencies ranged from approximately 10 to 100 times less than 17beta-estradiol. DBP was stronger than DEHP in the concentration of maximal efficacy. However, DEHP was stronger than DBP in the MCF-7 cell proliferation. Results from this study suggested that DEHP and DBP may play an important role in the estrogenic activity. Therefore, it is suggested that DEHP and DBP are estrogenic.
Keywords
DEHP; DBP; phthalate; MCF-7 cell;
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