Advances in the Analysis of Total Aflatoxins in Foods

We optimized conditions of extract solvents and elution solvents for total aflatoxins in foods using HPLC/FLD. The extract solvent was 70% methanol solution including 1% NaCl and the 3 mL of acetonitrile was used as elution solvent using immnuoaffinity column. The detection limits (LOD) was 0.05 ng/g. The recoveries for total aflatoxins ($B_1,\;B_2,\;G_1\;and\;G_2$) studied in foods were cereals ($74.1{\sim}95.5%,\;83.7{\sim}98.8%,\;80.4{\sim}102.4%,\;72.8{\sim}76.5%$), pulses ($85.8{\sim}87.5%,\;83.8{\sim}90.7%,\;92.0{\sim}94.5%,\;60.6{\sim}65.6%$), nuts ($84.6{\sim}97.1%,\;86.0{\sim}94.1%,\;95.5{\sim}111.5%,\;71.0{\sim}89.9%$), processed foods ($81.5{\sim}87.1%,\;82.8{\sim}85.8%,\;85.4{\sim}92.7%,\;68.9{\sim}76.4%$), dried fruits ($83.6{\sim}93.5%,\;78.1{\sim}90.4%,\;93.0{\sim}108.5%,\;64.9{\sim}78.5%$) and other foods ($72.5{\sim}98.3%,\;73.1{\sim}96.4%,\;83.5{\sim}107.2%,\;64.2{\sim}75.8%$), respectively.

식품중 총 아플라톡신 분석법 개선

총 아플라톡신 분석을 위한 최적의 추출 및 용출조건을 확립하여 최적의 전처리 조건을 확립하였다. 추출용매는 70% methanol 용액에 NaCl을 각각 1% 첨가한 용액을 사용하였고, 정제용 컬럼인 immunoaffinity column을 이용하여 acetonitrile 3 mL를 용출용매로 사용하여 아플라톡신 혼합용액의 분리능을 좋게 하였다. 검출한계는 0.05 ng/g 수준이였고, 식품의 유형별 총 아플라톡신($B_1,\;B_2,\;G_1,\;G_2$)의 회수율은 곡류 $74.1{\sim}95.5%,\;83.7{\sim}98.8%,\;80.4{\sim}102.4%$ 및 $72.8{\sim}76.5%$, 두류 $85.8~87.5%,\;83.8{\sim}90.7%,\;92.0{\sim}94.5%$ 및 $60.6{\sim}65.6%$, 견과류 $84.6{\sim}97.1%,\;86.0{\sim}94.1%,\;95.5{\sim}111.5%$ 및 $71.0{\sim}89.9%$, 가공식품 $81.5{\sim}87.1%,\;82.8{\sim}85.8%,\;85.4{\sim}92.7%$ 및 $68.9{\sim}76.4%$, 건조과실류 $83.6{\sim}93.5%,\;78.1{\sim}90.4%,\;93.0{\sim}108.5%$ 및 $64.9{\sim}78.5%$, 기타식품 $72.5{\sim}98.3%,\;73.1{\sim}96.4%,\;83.5{\sim}107.2%$ 및 $64.2{\sim}75.8%$를 보여주었다.