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Primers for Typing Salmonella spp. using Random Amplified Polymorphic DNA (RAPD) Analysis  

Lim, Hyung-Kum (Department of Pharmacology, Pusan National University)
Lee, Kyung-Hee (Department of Pharmacology, Pusan National University)
Hong, Chong-Hae (Department of Veterinary Medicine, Kangwon National Unversity)
Park, Gyung-Jin (HACCP Team, Korea Health Industry Develpment Institute)
Choi, Weon-Sang (Department of Biotechnology, Dongguk University)
Publication Information
Journal of Food Hygiene and Safety / v.18, no.4, 2003 , pp. 224-228 More about this Journal
Abstract
Random amplified polymorphic DNA (RAPD) analysis is based on the amplification of random DNA segment using a single arbitratrary primer. For typing Salmonella spp., polymorphic DNA patterns identified by this method can be used. To select the primers for RAPD typing Salmonella spp., the performances of 20 primers were compared by analyzing 16 Salmonella spp. reference strains. Reproducible electrophoresis patterns were obtained. Among the 20 primers tested, 4 primers (A, OPG04, OPG10, OPL03) showed better differentiation than the others. At the time discrimination index, band clarity, band number and difficulty of band scoring were considered. These primers will be useful for typing Salmonella spp. in the future. Curretly, we are under investigation for the RAPD typing of contaminated Slmonella spp. for the risk analysis of pork processing plant using the primers.
Keywords
Random amplified polymorphic DNA; RAPD; Salmonella spp.;
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