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Production of Monoclonal Antibody for Listeria spp. p60 Protein Based on iap Gene  

임희영 (경기대학교 자연과학부 생물학과 유전학실험실)
오연경 (경기대학교 자연과학부 생물학과 유전학실험실)
김종수 (경기대학교 자연과학부 생물학과 유전학실험실)
이영순 (서울대학교 수의과대학 공중보건학실험실)
임윤규 (제주대학교 수의과대학 공중보건학실험실)
윤병수 (경기대학교 자연과학부 생물학과 유전학실험실)
Publication Information
Journal of Food Hygiene and Safety / v.18, no.1, 2003 , pp. 25-29 More about this Journal
Abstract
The p60 protein of Listeria spp. is a Listeria-Genus-specific, major extra-cellular protein, which is used as an indicator protein for the detection of these bacteria from contaminated foods. In this study, p60 protein were recombinantly produced in E. coli and were purified using amylose resin based column chromatography. Purified recombinant-p6O was used to generate monoclonal antibody against native p60. Antibody from hybridoma cell line, 1H4, specificically reacted with native p60 protein isolated from pathogenic Listeria spp. such as L. monocytogenes, L. ivanovii, L. welshimeri II, but did not or relatively weakly reacted with non-pathogenic Listeia species, L. innocua or other bacterial proteins. Antibody from 1H4 was produced using ascites fluid method and it may be useful to develop the Listeria-detection kits based on immunological method.
Keywords
Listeria spp.; p60 protein; hybridoma cell; pathogenic;
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