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In vitro Screening of Natural Product Inhibitors on the UVB Phototoxicity  

김현진 (한국화학연구원 부설 안전성평가연구소)
이경아 (충남대학교 약학대학)
김봉희 (충남대학교 약학대학)
Publication Information
Journal of Food Hygiene and Safety / v.17, no.4, 2002 , pp. 188-192 More about this Journal
Abstract
The phototoxicity inhibitory activity of 15 natural products having antiinflammatory effect was screened by three in vitro methods : yeast growth inhibition test with Candida albicans, RBC photohemolysis and MTT assay. We induced phototoxic reaction by irradiating UVB (312 nm) on chlorpromazine (CPZ) that has been widely documented as phototoxic agent in clinical and experimental studies and then observed the effects of the natural products after treating them with CPZ. In yeast growth Inhibition test, P. persica and E. officinalis showed the inhibitory effect on the UVB phototoxicity and E. officinalis, yeast, P. suffruticosa showed phototoxicity inhibitory effect in that their ％ hemolysis compared with control were 45.76 $\pm$ 0.91, 34.42$\pm$1.01, 35.30 $\pm$4.76 on UVB. In MTT assay, all tested natural products increased cell viability compared with the contort.
Keywords
UVB phototoxicity inhibitors; Natural producets; Yeast growth inhibition; RBC photohemolysis; MTT assay; Chlorromazine;
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