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Antimicrobial Activity of GC-l00X against Major Food-Borne Pathogens and Detaching Effects of It against Escherichia coli O157:H7 on the surface of Tomatoes  

박용호 (서울대학교 농생명공학 사업단 수의대 미생물학교실)
권남훈 (서울대학교 농생명공학 사업단 수의대 미생물학교실)
김소현 (서울대학교 농생명공학 사업단 수의대 미생물학교실)
김지연 (서울대학교 농생명공학 사업단 수의대 미생물학교실)
임지연 (서울대학교 농생명공학 사업단 수의대 미생물학교실)
김준만 (서울대학교 농생명공학 사업단 수의대 미생물학교실)
정우경 (서울대학교 농생명공학 사업단 수의대 미생물학교실)
박건택 (서울대학교 농생명공학 사업단 수의대 미생물학교실)
배원기 (서울대학교 농생명공학 사업단 수의대 미생물학교실)
Publication Information
Journal of Food Hygiene and Safety / v.17, no.1, 2002 , pp. 36-44 More about this Journal
Abstract
GC-l00X is non-corrosive alkaline ionic water (pH 12). It is composed of hydroxyl radicals and supplemented with xylitol. Its antimicrobial activity was examined against 6 major food-borne pathogens; Staphylococcus aureus FRI 913, Salmonella enterica serova Enteritidis ATCC 13076, S. enterica serova Typhimurium Korean isolate, Vibrio parahaemolyticus ATCC 17803, Escherichia coli O157:H7 ATCC 43894 and Pseudomonas aeruginosa KCTC 1637 at three different temperatures (4$^{\circ}C$, $25^{\circ}C$ and 36$^{\circ}C$) with or without an organic material (2% yeast extract), respectively. The antimicrobial activities showed over 4 log-reductions (1.0$\times$10$^4$CFU/ml reduction) against all pathogens reacted at 37$^{\circ}C$ for 3 hours in the absence of the organic material. The activities showed same results when GC-l00X was diluted with same volume of distilled water or standard hard water (CaCO$_3$300 ppm). Its antimicrobial activity was more effective and quicker in Gram-negative bacteria than Gram-positive bacteria. Its washing efficacy against E. coli O157:H7 exposed to the surfaces of tomatoes (grapes) was compared with that of the other sanitizers such as other kitchen synthetic detergent and 100-ppm chlorine water. For the toxicological evaluation of the sanitizers, viable counts of E. coli O157:H7 penetrated into the core of tomatoes after washing products were also compared. The result revealed that GC-100X stock solution and its 5% diluted solution had similar washing effects to 100-ppm chlorine water and more effective than the other kitchen synthetic detergent. This result indicated that GC- l00X had antimicrobial activity and no toxicological side effects, therefore, could be useful for a new sanitizer to use in flood safety and kitchen hygiene.
Keywords
GC-100X; Food-borne pathogen; Produce wash; Tomatoes;
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