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Determination of Urinary Metabolite of Profenofos after Oral Administration and Dermal Application to Rats  

민경진 (계명대학교 공중보건학과)
조영주 (농업과학기술원 환경생태과)
이인선 (계명대학교 TMR센터)
차춘근 (계명대학교 공중보건학과)
Publication Information
Journal of Food Hygiene and Safety / v.17, no.1, 2002 , pp. 20-25 More about this Journal
Abstract
This study was aimed to determine the urinary metabolite of profenofos, one of the organophos-phorus pesticides, as the biomarkers of exposure. Urine samples were collected fort 24 hours in metabolic cages after oral administration and dermal application of profenofos to rats. Identification of the derivatized urinary metabolite was determined by GC/MS and excretion time courses of the urinary metabolite was analyzed by GC/MS. Urinary metabolite of profenofos, 4-bromo-2-chlorophenol, was detected in rats urine both after oral administration and dermal application of profenofos. Parent compound was not detected in the experiment. In GC/MS, the mass spectral confirmation for 4-bromo-2-chlorophenol ion was identified at m/z 208.4-bromo-2-chlorophenol was excreted within 48 hours and 72 hours after oral administration and dermal application of profenofos, respectively. In this study, the same urinary metabolite of profenofos was detected both in oral and dermal exposure. Generally, excretion of the urinary metabolite after oral administration was detected faster than after dermal application. It is suggested that urinary 4-bromo-2-chlorophenol could be used as the biomarkers of exposure to profenofos.
Keywords
Profenofos; Metabolite; Urine; GC/MS;
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