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http://dx.doi.org/10.9721/KJFST.2022.54.3.280

Antioxidant and whitening effects of loquat (Eriobotrya japonica) fruit extracts  

Yun, Min-Kyu (Department of Food Science and Biotechnology, Kyung Hee University)
Park, Gi-Cheol (Department of Food Science and Biotechnology, Kyung Hee University)
Cho, Youn-Sup (Jeollanamdo Agricultural Research and Extension Services)
Kim, Dae-Ok (Department of Food Science and Biotechnology, Kyung Hee University)
Publication Information
Korean Journal of Food Science and Technology / v.54, no.3, 2022 , pp. 280-287 More about this Journal
Abstract
The loquat (Eriobotrya japonica) is a fruit tree belonging to the Rosaceae family. Loquat fruit extracts from three cultivars (Tanaka, Mogi, and Jinwang) were prepared using absolute methanol and homogenization and ultrasound sonication procedures. We measured the total phenolic and flavonoid content of the fruit extracts and their antioxidant capacities. In addition, we evaluated tyrosinase activity and anti-melanogenic effects in B16F1 melanoma cells. The fruit extract from cv. Tanaka had the highest total phenolic content and showed the highest antioxidant capacity in the ABTS assay. The loquat fruit extract from cv. Jinwang had the highest total flavonoid content and showed the highest antioxidant capacity in the DPPH assay. Loquat fruit extracts from the Tanaka and Jinwang cultivars effectively inhibited mushroom tyrosinase activity. The loquat fruit extracts reduced intracellular oxidative stress in B16F1 melanoma cells. Treating B16F1 melanoma cells with loquat fruit extract from cv. Tanaka at a concentration of 125 ㎍/mL effectively inhibited melanin synthesis. However, treating the B16F1 melanoma cells with loquat fruit extracts from the Jinwang and Mogi cultivars did not. These results suggest that loquat fruit extracts from the Tanaka cultivar may serve as potential sources of antioxidants and act as a skin-whitening agent.
Keywords
antioxidant capacity; intracellular oxidative stress; loquat fruit; melanin; tyrosinase;
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1 Fu X, Feng C, Wang C, Yin X, Lu P, Grierson D, Xu C, Chen K. Involvement of multiple phytoene synthase genes in tissue-and cultivar-specific accumulation of carotenoids in loquat. J. Exp. Bot. 65: 4679-4689 (2014)   DOI
2 Garcia-Borron JC, Solano F. Molecular anatomy of tyrosinase and its related proteins: Beyond the histidine-bound metal catalytic center. Pigm. Cell Res. 15: 162-173 (2002)   DOI
3 Hong S-J, Kim K-J. Scutellaria baicalensis Georgi (SBG) inhibits melanin synthesis in mouse B16 melanoma cells. J. Korean Med. Ophthalmol. Otolaryngol. Dermatol. 22: 104-117 (2009)
4 Iwai K, Kishimoto N, Kakino Y, Mochida K, Fujita T. In vitro antioxidative effects and tyrosinase inhibitory activities of seven hydroxycinnamoyl derivatives in green coffee beans. J. Agric. Food Chem. 52: 4893-4898 (2004)   DOI
5 Jeong H-R, Baek Y, Kim D-O, Lee H. Antioxidative and antimelanogenic effects of ethyl acetate fractions of Korean domestic honeys from different floral sources. Korean J. Food Sci. Technol. 50: 660-664 (2018)   DOI
6 Jian D, Jiang D, Su J, Chen W, Hud X, Kuang Y, Xie H, Li J, Chen X. Diethylstilbestrol enhances melanogenesis via cAMP-PKA-mediating up-regulation of tyrosinase and MITF in mouse B16 melanoma cells. Steroids 76: 1297-1304 (2011)   DOI
7 Slominski RM, Sarna T, Plonka PM, Raman C, Brozyna AA, Slominski AT. Melanoma, melanin, and melanogenesis: The Yin and Yang relationship. Front. Oncol. 12: 842496 (2022)   DOI
8 Stark KB, Gallas JM, Zajac GW, Eisner M, Golab JT. Spectroscopic study and simulation from recent structural models for eumelanin: I. Monomer, dimers. J. Phys. Chem. 107: 3061-3067 (2003)   DOI
9 Valencia A, Moran J. Reactive oxygen species induce different cell death mechanisms in cultured neurons. Free Radic. Biol. Med. 36: 1112-1125 (2004)   DOI
10 Kim KJ, Jung YS, You DM, Lee SH, Lee G, Kwon K-B, Kim D-O. Neuroprotective effects of ethanolic extract from dry Rhodiola rosea L. rhizomes. Food Sci. Biotechnol. 30: 287-297 (2021)   DOI
11 Kim D-O, Lee CY. Considerations for the effects of antioxidant phytochemicals on human health. Korean J. Food Sci. Technol. 53: 111-114 (2021)   DOI
12 Li H-R, Habasi M, Xie L-Z, Aisa HA. Effect of chlorogenic acid on melanogenesis of B16 melanoma cells. Molecules 19: 12940-12948 (2014)   DOI
13 Whang T-E, Lim H-O, Lee J-W. Anticancer effect of Eriobotrya japonica Lindl by specificity test with several cancer cell lines. Korean J. Med. Crop Sci. 4: 310-320 (1996)
14 Yoo J-M, Kang Y-J, Pyo H-B, Choung ES, Park SY, Choi JH, Han G-J, Lee CH, Ki T-J. Anti-wrinkle effects of Korean rice wine cake on human fibroblast. J. Life Sci. 20: 1838-1843 (2010)   DOI
15 Yoo KM, Kim D-O, Lee CY. Evaluation of different methods of antioxidant measurement. Food Sci. Biotechnol. 16: 177-182 (2007)
16 Lee B-Y, Park E-M, Kim E-J, Choi H-D, Kim I-H, Hwang J-B. Analysis of chemical components of Korean loquat (Eriobotrya japonica Lindl.) fruit. Korean J. Food Sci. Technol. 28: 428-432 (1996)
17 Kubglomsong S, Theerakulkait C, Reed RL, Yang L, Maier CS, Stevens JF. Isolation and identification of tyrosinase-inhibitory and copper-chelating peptides from hydrolyzed rice-bran-derived albumin. J. Agric. Food Chem. 66: 8346-8354 (2018)   DOI
18 Kwon E-J, Park H-J, Kim M-M, Lee KR, Hong I, Lee DG, Oh Y. Effect of Ulmus macrocapa ethanolic extracts on anti-oxidant activity and melanin synthesis in B16F1 cells. J. Life Sci. 24: 946-951 (2014)   DOI
19 Lee K-I, Kim S-M. Antioxidative and antimicrobial activities of Eriobotrya japonica Lindl. leaf extracts. J. Korean Soc. Food Sci. 38: 267-273 (2009)   DOI
20 Lee H-J, Lee M-K, Park I-S. Characterization of mushroom tyrosinase inhibitor in sweet potato. J. Life Sci. 16: 369-399 (2006)   DOI
21 Park M-Y, Lee S-M, Cho H-S, Park J-O, Kim B-S, Cho K-C, Jeong H-J, Jeong B-J, Cho Y-S, Lee J-H, Choi D-S, Ma K-C, Ko S-J. Loquat Cultivation Manual. Fruit Research institute, Jeollanamdo Agricultural Research and Extension Services, Wando, South Korea (2017)
22 Cory H, Passarelli S, Szeto J, Tamez M, Mattei J. The role of polyphenols in human health and food systems: A mini-review. Front. Nutr. 5: 87 (2018)   DOI
23 Eom T, Woo K, Shim BS. Melanin: A naturally existing multifunctional material. Appl. Chem. Eng. 27: 115-122 (2016)   DOI
24 Heo H-j, Cho H-Y, Hong B, Kim H-K, Kim E-K, Kim B-G, Shin DH. Protective effect of 4',5-dihydroxy-3',6,7-trimethoxyflavone from Artemisia asiatica against Aβ-induced oxidative stress in PC12 cells. Amyloid-J. Protein Fold. Disord. 8: 194-201 (2001)   DOI
25 Kim EJ, Choi JY, Yu M, Kim MY, Lee S, Lee B-H. Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants. Korean J. Food Sci. Technol. 44: 337-3425 (2012)   DOI
26 Lim Y-J, Lee EH, Kang TH, Ha SK, Oh MS, Kim SM, Yoon T-J, Kang C, Park J-H, Kim SY. Inhibitory effects of arbutin on melanin biosynthesis of α-melanocyte stimulating hormone-induced hyperpigmentation in cultured brownish guinea pig skin tissues. Arch. Pharm. Res. 32: 367-373 (2009)   DOI
27 Masaki H, Sakaki S, Atsumi T, Sakurai H. Active-oxygen scavenging activity of plant extracts. Biol. Pharm. Bull. 18: 162-166 (1995)   DOI
28 Obaid RJ, Mughal EU, Naeem N, Sadiq A, Alsantali RI, Jassas RS, Moussa Z, Ahmed SA. Natural and synthetic flavonoid derivatives as new potential tyrosinase inhibitors: A systematic review. RSC Adv. 11: 22159-22198 (2021)   DOI
29 Pareek S, Benkeblia N, Janick J, Cao S, Yahia EM. Postharvest physiology and technology of loquat (Eriobotrya japonica Lindl.) fruit. J. Sci. Food. Agric. 94: 1495-1504 (2014)   DOI
30 Park YS, Park HS. Changes in cold injury and contents of chemical compounds as related the different growth stage of immature loquat fruit. J. Korean Soc. Hort. Sci. 36: 522-534 (1995)
31 Badenes ML, Martinez-Calvo J, Llacer G. Analysis of a germplasm collection of loquat (Eriobotrya japonica Lindl.). Euphytica 114: 187-194 (2000)   DOI
32 Chang T-S. An updated review of tyrosinase inhibitors. Int. J. Mol. Sci. 10: 2440-2475 (2009)   DOI
33 Cho S. Pathogenesis and prevention of skin aging. J. Korean Med. Assoc. 64: 438-446 (2021)   DOI
34 Cho H-S, Kim K-H. Quality characteristics of Maejakgwa containing various levels of Eriobotrya japonica leaf powder. J. East Asian Soc. Diet. Life 8: 550-557 (2012)
35 Panzella L, Napolitano A. Natural and bioinspired phenolic compounds as tyrosinase inhibitors for the treatment of skin hyperpigmentation: Recent advances. Cosmetics 6: 57 (2019)   DOI
36 Park Y-S, Park M-Y, Jo Y-S. Storability of loquat fruits as influenced by harvest date and storage temperature. Korean J. Hort. Sci. Technol. 23: 64-70 (2005)
37 Park KJ, Park SH, Kim JK. Anti-wrinkle activity of Acanthopanax senticosus extract in ultraviolet B (UVB)-induced photoaging. J. Korean. Soc. Food. Sci. Nutr. 39: 42-46 (2010)   DOI
38 Park SA, Park J, Park CI, Jie YJ, Hwang YC, Kim YH, Jeon SH, Lee HM, Ha JH, Kim KJ, Park SN. Cellular antioxidant activity and whitening effects of Dendropanax morbifera leaf extracts. Korean J. Microbiol. Biotechnol. 41: 407-415 (2013)   DOI
39 Shaw PE, Wilson CW. Determination of organic acids and sugars in loquat (Eriobotrya japonica Lindl.) by high-pressure liquid chromatography. J. Sci. Food. Agric. 32: 1242-1246 (1981)   DOI
40 Sim JY, Lee J-H. A possible protective role of Ginko biloba outer seed coat methanol extracts on DNA damage inducedby H2O2 in HaCaT human skin keratinocytes. J. Life Sci. 29: 1164-1170 (2019)
41 Dawidowicz, L. A, Olszowy, Malgorzata. The importance of solvent type in estimating antioxidant properties of phenolic compounds by ABTS assay. Eur. Food Res. Technol. 236: 1099-1105 (2013)   DOI
42 Ding C-K, Chachin K, Ueda Y, Imahori Y, Kurooka H. Effects of high CO2 concentration on browning injury and phenolic metabolism in loquat fruits. J. Jpn. Soc. Hort. Sci. 68: 275-282 (1999)   DOI
43 Ding C-K, Chachin K, Ueda Y, Imahori Y, Wang CY. Metabolism of phenolic compounds during loquat fruit development. J. Agric. Food Chem. 49: 2883-2888 (2001)   DOI
44 Fan M, Zhang G, Hu X, Xu X, Gong D. Quercetin as a tyrosinase inhibitor: Inhibitory activity, conformational change and mechanism. Food Res. Int. 100: 226-233 (2017)   DOI
45 Cho Y-S, Park S-K, Lee H-Y. Composition of free sugars, organic acids and free amino acids in loquat flesh. J. Korean Soc. Food Nutr. 20: 89-93 (1991)
46 Fernandez AF, Fraga MF. The effects of the dietary polyphenol resveratrol on human healthy aging and lifespan. Epigenetics 6: 870-874 (2011)   DOI
47 Fruehauf J, Meyskens F. Reactive oxygen species: A breath of life or death? Clin. Cancer Res. 13: 789-794 (2007)   DOI
48 Park CH, Park JH, Min SY, Kim K, Kim S, Park YJ. Studies on antioxidant, anti-inflammation and whitening activities of Hordeum vulgare L. extracts and their fractions. J. Soc. Cosmet. Sci. Korea 45: 287-297 (2019)   DOI
49 Zhang W, Zhao X, Sun C, Li X, Chen K. Phenolic composition from different loquat (Eriobotrya japonica Lindl.) cultivars grown in China and their antioxidant properties. Molecules 20: 542-555 (2015)   DOI