Browse > Article
http://dx.doi.org/10.9721/KJFST.2022.54.2.155

Immunostimulatory effects of enzymatic porcine placental hydrolyzate against cyclophosphamide-induced immunosuppressed model  

Kim, Keun Nam (Unimed Pharm Inc.)
Kim, Min Ju (Unimed Pharm Inc.)
Yoon, Sun Myung (Unimed Pharm Inc.)
Kwon, Min Joo (Invivo Co. Ltd.)
Shin, Dong Yeop (Invivo Co. Ltd.)
Lee, Hak Yong (Invivo Co. Ltd.)
Park, Young Mi (Invivo Co. Ltd.)
Publication Information
Korean Journal of Food Science and Technology / v.54, no.2, 2022 , pp. 155-162 More about this Journal
Abstract
This study investigated the immunostimulatory effect of enzymatic porcine placental hydrolyzate (EPPH) in cyclophosphamide (Cy)-treated rats. This effect of EPPH prevented Cy-induced decreases in body, spleen, and thymus weights and natural killer (NK) cell activity. The numbers of immune cells, such as white blood cells, granulocytes, and lymphocytes, and mid-range absolute counts were significantly higher compared to the control group. The levels of tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, IL-2, IL-12, and immunoglobulin G (IgG) were notably reduced by Cy, while EPPH prevented these effects. Histopathological analysis of spleen samples revealed the protective effect of EPPH against Cy-induced immunosuppression. The findings demonstrate that EPPH can alleviate immunosuppression by cell viability, tissue damage, and regulation of the levels of cytokines. EPPH may have value as a component of immunostimulatory agents or an ingredient in functional foods.
Keywords
enzymatic porcine placenta hydrolysate; immunodeficiency animal model; cyclophosphamide; cytokine; immunostimulation;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Banerjee KK, Bishayee A, Chatterjee M. Anti-inflammatory effect of human placental extract: a biochemical mechanistic approach. Riv. Eur. Sci. Med. Farmacol. 14: 361-366 (1992)
2 Cesta MF. Normal structure, function, and histology of the spleen. Toxicol. Pathol. 34: 455-465 (2006)   DOI
3 Choi JH, Lee EB, Park YG, Lee HK, Jang HH, Choe JS, Hwang KA, Park SY, Hwang IG, Hong HC, Lee HJ, Jeong HC, Lim HJ, Lee SH. Aged doraji (Platycodon grandiflorum) ameliorates cyclophosphamide-Induced immunosuppression in mice. Kor. J. Pharmacogn. 50: 219-225 (2019)
4 Davis L, Kuttan G. Effect of Withania somnifera on cytokine production in normal and cyclophosphamide treated mice. Immunopharmacol. Immunotoxicol. 21: 695-703 (1999)   DOI
5 Han NR, Kim KY, Kim MJ, Kim MH, Kim HM, Jeong HJ. Porcine placenta mitigates protein-energy malnutrition-induced fatigue. Nutrition. 29: 1381-1387 (2013)   DOI
6 Hong SH, Kwon MJ, Lee HY, Park YM, Shin DY, Choi JS, Kim MJ, Yang HJ, Shin NR. Immunomodulatory effect of fermented vinegar on cyclophosphamide-induced immunosuppression model. J. Food Nutr. Res. 9: 469-476 (2021)   DOI
7 Hussain A, Shadma W, Maksood A, Ansari SH. Protective effects of Picrorhiza kurroa on cyclophosphamide-induced immunosuppression in mice. Pharmacognosy Res. 5: 30-35 (2013)   DOI
8 Kiladjian JJ, Bourgeois E, Lobe I, Braun T, Visentin G, Bourhis JH, Fenaux P, Chouaib S, Caignard A. Cytolytic function and survival of natural killer cells are severely altered in myelodysplastic syndromes. Leukemia. 20: 463-470 (2006)   DOI
9 Kim JH, Lee JW, Kim YI, Lee MH. The effect of placental extract on the expression of tyrosinase, TRP-1 and TRP-2 in SK30 melanoma cells. J. Kor. Derma. Assoc. 41: 1612-1618 (2003b)
10 Chen F, Huang G. Preparation and immunological activity of polysaccharides and their derivatives. Int. J. Biol. Macromo. 7: 211-216 (2018)   DOI
11 Taguchi T. Induction chemotherapy for solid tumors. Gan To Kagaku Ryoho. 40: 679-683 (2013)
12 Ozkok A, Kaymaz S, Elcioglu OC, Bakan A, Odabas AR. Cyclophosphamide induced early-onset interstitial lung disease. CEN Case Rep. 1: 128-129 (2012)   DOI
13 Rasmussen L, Arvin A. Chemotherapy-induced immunosuppression. Environ. Health Perspect. 43: 21-25 (1982)   DOI
14 Shalit I, Kletter Y, Halperin D, Waldman D, Vasserman E, Nagler A, Fabian I. Immunomodulatory effects of moxifloxacin in comparison to ciprofloxacin and G-CSF in a murine model of cyclophosphamide- induced leukopenia. Eur. J. Haematol. 66: 287-296 (2001)   DOI
15 Wang K, Conlon M, Ren W, Chen BB, Baczek T. Natural products as targeted modulators of the immune system. J. Immunol. Res. 7862782 (2018)   DOI
16 Zhang M, Liu X, Li J, He L, Tripathy D. Chinese medicinal herbs to treat the side-effects of chemotherapy in breast cancer patients. Cochrane Data. Syst. Rev. 18: CD004921 (2007)
17 Bourke CD, Berkley JA Prendergast AJ. Immune dysfunction as a cause and consequence of malnutrition. Trends Immunol. 37: 386-398 (2016)   DOI
18 Chen XG, Huang H, Tian Y, Guo CC, Liang CY, Gong YL, Zou BY, Cai RQ, Lin TY. Cyclosporine, prednisone, and high-dose immunoglobulin treatment of angioimmunoblastic T cell lymphoma refractory to prior CHOP or CHOP-like regimen. Chin. J. Cancer. 30: 731-738 (2011)   DOI
19 Haddad PS, Azar GA, Groom S, Boivin M. Natural health products, modulation of immune function and prevention of chronic diseases. Evid. Based Complement. Alternat. Med. 2: 513-520 (2005)   DOI
20 Hoffman WP, Ness DK, Van Lier RB. Analysis of rodent growth data in toxicology studies. Toxicol. Sci. 66: 313-319 (2002)   DOI
21 Mitsui Y, Bagchi M, Marone PA, Moriyama H, Bagchi D. Safety and toxicological evaluation of a novel, fermented, peptideenriched, hydrolyzed swine placenta extract powder. Toxicol. Mech. Methods. 25: 13-20 (2015)   DOI
22 Yu Q, Nie SP, Wang JQ, Liu XZ, Yin PF, Huang DF, Li WJ, Gong DM, Xie MY. Chemoprotective effects of Ganoderma atrum polysaccharide in cyclophosphamide-induced mice. Int. J. Biol. Macromol. 64: 395-401 (2014)   DOI
23 Zhou Y, Chen X, Yi R, Li G, Sun P, Qian Y, Zhao X. Immunomodulatory effect of Tremella polysaccharides against cyclophosphamide-induced immunosuppression in Mice. Molecules 23: 239 (2018)   DOI
24 Jeong DY, Yang HJ, Jeong SJ, Shin DY, Lee HY, Park YM. Immunoregulatory activities of blueberry yeast fermented powder in immunosuppressed model. J. Korean Soc. Food Sci. Nutr. 49: 433-443 (2020)   DOI
25 Kotas, M.E. Medzhitov R. Homeostasis, inflammation, and disease susceptibility. Cell. 160: 816-827 (2015)   DOI
26 Lin ZB, Zhang HN. Anti-tumor and immunoregulatory activities of Ganoderma lucidum and its possible mechanisms. Acta. Pharmacol. Sin. 25: 1387-1395 (2004)
27 Miyauchi A. Hiramine C, Tanaka S. Hojo, K. Differential effects of a single dose of cyclophosphamide on T cell subsets of the thymus and spleen in mice: flow cytofluorometry analysis. Tohoku J. Exp. Med. 162: 147-167 (1990)   DOI
28 Kumar S, Kanti B, Liaquat A, Biswapati M. Anti-inflammatory and antiplatelet aggregation activity of human placental extract. Act. Pharmacol. Sini. 24: 187-192 (2003)
29 Kumar S, Sharma G, Sidiq T, Khajuria A, Jain M, Bhagwat D, Dhar KL. Immunomodulatory potential of a bioactive fraction from the leaves of Phyllostachys bambusoides (bamboo) in BALB/c mice. EXCLI J. 13: 137-150 (2014)
30 McKallip RJ, Lombard C, Martin BR, Nagarkatti M, Nagarkatti PS. Delta(9)-tetrahydrocannabinol-induced apoptosis in the thymus and spleen as a mechanism of immunosuppression in vitro and in vivo. J. Pharmacol. Exp. Ther. 302: 451-465 (2002)   DOI
31 Xun CQ. Thornpson JS, Jennings CD, Brown SA, Widrner MB. Effects of total body irradiation, busulfan-cyclophosphamide, or cyclophosphamide conditioning on inflammatory cytokine release and development of acute and chronic graft versus-host disease in H-2-incompatible transplanted SCID mice. Blood 83: 2360-2367 (1994)   DOI
32 Han NR, Park CL, Kim NR, Kim HY, Yoou MS, Nam SY, Moon PD, Jeong HJ, Kim HM. Protective effect of porcine placenta in a menopausal ovariectomized mouse. Reproduction. 150: 173-181 (2015)   DOI
33 Kim DW, Chung KT. Ulmus macrocarpa hance reduces cyclophosphamide- induced toxicity in mouse liver. J. Life Sci. 31: 223-228 (2021)   DOI
34 Kim HJ, Lee JW, Kim YL, Lee MH. The effect of placental extract on the expression of tyrosinase, TRP-1 and TRP-2 in SK30 melanoma cells. Korean J. Dermatol. 41: 1612-1618 (2003a)
35 Rehman MU, Tahir M, Ali F, Qamar W, Lateef A, Khan R, Quaiyoom A, Hamiza OO, Sultana S. Cyclophosphamide-induced nephrotoxicity, genotoxicity, and damage in kidney genomic DNA of Swiss albino mice: the protective effect of Ellagic acid. Mol. Cell Biochem. 365: 119-127 (2012)   DOI
36 Noh EM, Kim JM, Lee HY, Song HK, Joung SO, Yang HJ, Kim MJ, Lim KS, Lee YR. Immuno-enhancement effects of Platycodon grandiflorum extracts in splenocytes and a cyclophosphamide induced immunosuppressed rat model. BMC Complement. Altern. Med. 19: 322 (2019)   DOI
37 Ooi VE, Liu F. Immunomodulation and anti-cancer activity of polysaccharide- protein complexes. Curr. Med. Chem. 7: 715-729 (2000)   DOI
38 Koo SW, Lim MK, Lee KW. Immunomodulatory effects of Zingiber officinale in cyclophosphamide-induced immunosuppress mice. J. Vet. Clin. 32: 56-61 (2015)   DOI
39 Park YM, Lee HY, Shin DY, Lee YH, Yang YJ, Lee HS, Lee JO, Choi KS, Kang JH, Cho YH, Kim MG, Yun CY, Kim MJ, Jang DJ, Yang HJ, Lee YR. Immunostimulatory activity of black rice bran in cyclophosphamide-induced immunosuppressed rats. Nat. Prod. Commun. 15: 1-11 (2020)
40 Pizzolo G, Trentin L, Vinante F, Agostini C, Zambello R, Masciarelli M, Feruglio C, Dazzi F, Todeschini G, Chilosi M, Veneri D, Zanotti R, Benedetti F, Perona G, Semenzato G. Natural killer cell function and lymphoid subpopulations in acute non-lymphoblastic leukaemia in complete remission. Br. J. Cancer. 58: 368-372 (1988)   DOI
41 Rosenthal M. The application of an extract of human placenta in the treatment of rheumatic affections. Int. J. Tiss. Reac. 4: 147-151 (1982)
42 Son CG, Han SH, Cho JH, Shin JW, Cho CH, Lee YW. Induction of hemopoiesis by saenghyuldan, a mixture of Ginseng radix, Paeoniae radix alba, and Hominis placenta extracts. Acta. Pharmacol. Sin. 24: 120-126 (2003)
43 Tiwary S, Shukla D, Tripathi A, Agrawal S, Singh M, Shukla V. Effect of placental-extract gel and cream on non-healing wounds. J. Wound Care. 15: 325-328 (2006)   DOI
44 Togashi, S., Takahashi, N., Iwama, M., Watanabe, S., Tamagawa, K., and Tetsuya, F. Antioxidative collagen-derived peptides in humanplacenta extract. Placenta 23: 497-502 (2002)   DOI
45 Shokrzadeh M, Chabra A, Ahmadi A, Naghshvar F, Habibi E, Salehi F, Assadpour S. Hepatoprotective effects of Zataria multiflora ethanolic extract on liver toxicity induced by cyclophosphamide in mice. Drug Res. 65: 169-175 (2015)   DOI