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http://dx.doi.org/10.9721/KJFST.2022.54.2.134

Anti-cancer effects of kelp extract in mouse melanoma B16-F0 cell line through apoptosis  

Lee, Seong-Uk (Department of Food and Nutrition, Daegu University)
Kim, Yoon Hee (Department of Food and Nutrition, Daegu University)
Publication Information
Korean Journal of Food Science and Technology / v.54, no.2, 2022 , pp. 134-140 More about this Journal
Abstract
Kelp belongs to the brown algae family and has been reported to exert anti-cancer effects on some cancer types, however studies have not been reported on the anti-cancer effects of kelp extracts on melanoma. In this study, the anti-cancer effects of kelp extract in B16-F0 cells were investigated, and the underlying molecular mechanisms were assessed. Kelp extract was found to inhibit the proliferation of B16-F0 cells, induce cytotoxicity, inhibit cell colony formation, and induce DNA fragmentation and apoptosis. The molecular mechanism was found to involve kelp extract increasing the expression of cytochrome-c and activated caspase-9 in the intrinsic apoptotic pathway. In addition, kelp extract upregulated the expression of Fas-associated protein with death domain and activated caspase-8 in the extrinsic apoptosis pathway. Activation of caspase-9 and caspase-8 by kelp extract induced activation of caspase-3 and cleaved poly adenosine diphosphate-ribose polymerase, consequently inducing apoptosis. These data suggest that kelp extract represents a potential therapeutic agent for melanoma.
Keywords
anti-cancer; apoptosis; kelp extract; melanoma;
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1 Wang Y, Sun HJ, Li RG, Wang XM, Cheng ZQ, Lou N. Reprogramming factors induce proliferation and inhibit apoptosis of melanoma cells by changing the expression of particular genes. Mol. Med. Rep. 19: 967-973 (2019)
2 Grozdanic N, Djuricic I, Kosanic M, Zdunic G, Savikin K, Etahiri S, et al. Fucus spiralis extract and fractions: anticancer and pharmacological potentials. JBUON. 25: 1219-1229 (2020)
3 Kang KS, Nam CS, Park EK, Ha BJ. The enzymaticn regulatory effects of laninaria japonica fucoidan extract in hepatotoxicity. J. Life Sci. 16: 1104-1108 (2006)   DOI
4 Lecoeur H, Ledru E, Prevost MC, Gougeon ML. Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISNT, annexin-V and 7-AAD cytofluotometric staining methods. J. Immunol. Methods. 209: 111-123 (1997)   DOI
5 Matassov D, Kagan T, Leblanc J, Sikorska M, Zakeri Z. Measurement of apoptosis by DNA fragmentation. Methods Mol. Biol. 282: 1-17 (2004)
6 Cai F, Xu H, Cao N, Zhang X, Liu J, Lu Y, et al. ADT-OH, a hydrogen sulfide-releasing donor, induces apoptosis and inhibits the development of melanoma in vivo by upregulating FADD. Cell Death Dis. 11: 33 (2020)   DOI
7 Chen G, Davies MA. Targeted therapy resistance mechanisms and therapeutic implications in melanoma. Hematol Oncol Clin. N. 28: 523-536 (2014)   DOI
8 Cui CB, Cho MA, Jun YY, Lee DS, Ham SS. Antimutagenicity and cytotoxicity effect of ethanol extract from korea traditional mackjang added sea tangle. J. East Asian Soc. Dietary Life. 12: 15-22 (2002)
9 Decker T, Lohmann-Matthes ML. A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity. J. Immunol. Methods. 115: 61-69 (1988)   DOI
10 Fan B, Dewapriya P, Li F, Blumel M, Tasdemir D. Pyrenosetins A-C, New Decalinoylspirotetramic Acid Derivatives Isolated by Bioactivity-Based Molecular Networking from the Seaweed-Derived Fungus Pyrenochaetopsis sp. FVE-001. Mar. Drugs. 18: 47 (2020)   DOI
11 Holohan C, Schaeybroeck SV, Longley DB, Johnston PG. Cancer drug resistance: an evolving paradigm. Nat. Rev. Cancer. 13: 714-726 (2013)   DOI
12 Jung SH, Ku MJ, Moon HJ, Yu BC, Jeon MJ, Lee YH. Inhibitory effects of fucoidan on melanin synthesis and tyrosinase activity. J. Life Sci. 19: 75-80 (2009)   DOI
13 Kim SH, Chung SH. Comparison of high dose interferon-α2b immunotherapy and dacarbazine chemotherapy as postoperative treatment of malignant melanoma. J. Korean Orthop. Assoc. 51: 426-431 (2016)   DOI
14 Rigel DS, Carucci JA. Malignant melanoma: prevention, early detection, and treatment in the 21st century. CA Cancer J. Clin. 50: 215-236 (2000)   DOI
15 Shalini S, Dorstyn L, Dawar S, Kumar S. Old, new and emerging functions of caspases. Cell Death. Differ. 22:526-539 (2014)   DOI
16 Rampal G, Khanna N, Thind TS, Vig AP. Role of isothiocyanates as anticancer agents and their contributing molecular and cellular mechanisms. Med. Chem. Drug Disco. 3: 79-93 (2012)
17 Rigel DS, Russak J, Friedman R. The evolution of melanoma diagnosis: 25 years beyond the ABCDs. CA Cancer J. Clin. 60: 301-316 (2010)   DOI
18 Raineri A, Fasoli S, Campagnari R, Gotte G, Menegazzi M. Onconase restores cytotoxicity in dabrafenib-resistant A375 human melanoma cells and a?ects cell migration, invasion and colony formation capability. Int. J. Mol. Sci. 20: 5980 (2019)   DOI
19 Brentnall M, Rodriguez ML, Guevara RLD, Cepero E, Boise LH. Caspase-9, caspase-3 and caspase-7 have distinct roles during intrinsic apoptosis. BMC Cell Biol. 14: 32 (2013)   DOI
20 Kang SY, Kim E, Kang I, Lee M, Lee Y. Anti-diabetic effects and anti-inflammatory effects of Laminaria japonica and Hizikia Fusiforme in skeletal muscle: In vitro and In vivo Model. Nutrients. 10: 491 (2018)   DOI
21 Kim SA, Kim J, Woo MK, Kwak CS, Lee MS. Antimutagenic and cytotoxic effects of ethanol extracts from five kinds of seaweeds. J. Korean Soc. Food Sci. Nutr. 34: 451-459 (2005)   DOI
22 Lee YS, Kim DS, Ryu BH, Lee SH. Antitumor and immunomodulating effects of seaweeds toward sarcoma-180 cell. J. Korean Soc. Food Sci. Nutr. 21: 544-550 (1992)
23 Lens MB, Dawes M. Global perspectives of contemporary epidemiological trends of cutaneous malignant melanoma. Brit. J. Dermatol. 150: 179-185 (2004)   DOI
24 Moussvou G, Kwak DH, Obiang OBW, Maranguy CAO, Dinzouna BSD, Lee DH, et al. Anticancer effects of different seaweeds on human colon and breasst cancers. Marine Drugs. 12: 4898-4911 (2014)   DOI
25 Nazarian R, Shi H, Wang Q, Kong X, Koya RC, Lee H, et al. Melanomas acquire resistance to B-RAF(V600E) inhibition by RTK or N-RAS upregulation. Nature. 468: 973-977 (2010)   DOI
26 Oh CM, Cho H, Won YJ, Kong HJ, Roh YH, Jeong KH, et al. Nationwide trends in the incidence of melanoma and non- melanoma skin cancers from 1999 to 2014 in South Korea. Cancer Res. Treat. 50: 729-737 (2018)   DOI
27 Ma ZY, Qiao X, Xie CZ, Shao J, Xu JY, Qiang ZY, et al. Activities of a novel Schiff Base copper (II) complex on growth inhibition and apoptosis induction toward MCF-7 human breast cancer cells via mitochondrial pathway. J. Inorg. Biochem. 117: 1-9 (2012)   DOI
28 Escring AJ, Goni CI. Nutritional Evaluation and Physiological Effects of Edible Seaweeds. Arch. Latinoam Nutr. 49: 114-120 (1999)
29 Hur SS, Jung JY, Park YH, Joo GJ, Choi YH. Studies on the physical properties of sea tangl extracts by the different extract methods. Agric. Res. Bull. Kyungpook Natl. Uni. 17: 79-83 (1999)
30 Jeon YE, Yim XF, Lim SS, Chung CK, Kang IJ. Antioxidant activities and acetylcholinesterase inhibitory activities from seaweed extracts, J. Korean Soc. Food Sci. Nutr. 41: 443-449 (2012)   DOI
31 Nakashima H, Kido Y, Kobayashi N, Motoki Y, Neushul M, Yamamoto N. Purification and characterization of an avian myeloblastosis and human immunodeficiency virus reverse transcriptase inhibitorsulfated polysaccharide extracted from sea algae. Antimicrob. Agents. CH. 31: 1524-1528 (1987)   DOI
32 Choo KS, Lee HN, Shin SA, Kim HJ, Park YS, Kim SK, et al. Induction of growth inhibition and apoptosis in human cancer cells by a brown algae extract. J. Life Sci. 26: 555-562 (2016)   DOI
33 Bae TJ, Kang DS. Effects of blood-anticoagulant activity of fucoidan extracted from sporophylls of Undaria pinnatifida. Bull. Fish. Sci. Inst. Chonnam Natl. Univ. 21: 24-31 (2013)
34 Bhatia S, Tykodi SS, Thompson JA. Treatment of metastatic melanoma. An overview. Oncology. 23: 488-495 (2009)
35 Blank V, Bellizzi Y, Zotta E, Cornier PG, Delpiccolo CML, Boggian DB, et al. A novel penicillin derivative induces antitumor effect in melanoma cells. Anti-cancer Drug. 29: 416-428 (2018)   DOI
36 Tummers B, Green DR. Caspase-8; regulating life and death. Immunol. Rev. 277: 76-89 (2017)   DOI
37 Easwaran H, Tsai HC, Baylin SB. Cancer epigenetics: tumor heterogeneity, plasticity of stem-like states, and drug resistance. Mol. Cell. 54: 716-727 (2014)   DOI
38 Tourneur L, Buzyn A, Chiocchia G. FADD adaptor in cancer. Med. Immunol. 4: 1-9 (2005)   DOI