Browse > Article
http://dx.doi.org/10.9721/KJFST.2021.53.2.115

Anti-inflammatory activity of Scutellaria Baicalensis root extract in lipopolysaccharide-induced RAW 264.7 cells  

Lee, Ye Eun (Department of Efficacy Study, Institute of Jinan red ginseng)
Park, Hong Jin (Department of Efficacy Study, Institute of Jinan red ginseng)
Park, Chung-berm (Department of Efficacy Study, Institute of Jinan red ginseng)
Hwang, Seung-mi (Department of Efficacy Study, Institute of Jinan red ginseng)
Publication Information
Korean Journal of Food Science and Technology / v.53, no.2, 2021 , pp. 115-120 More about this Journal
Abstract
Scutellaria baicalensis has been used as a traditional medicine for diarrhea, dysentery, hypertension, hemorrhaging, insomnia, inflammation, and respiratory infections. This study examined the anti-inflammatory effect of Scutellaria baicalensis water extract (SWE) in lipopolysaccharide (LPS)-induced RAW 264.7 cells. To evaluate the anti-inflammatory effect of SWE, RAW 264.7 macrophages were stimulated with LPS to induce the production of inflammation-related factors, which were measured by western blotting. In RAW 264.7 cells, SWE inhibited the production of nitric oxide (NO) without causing cell toxicity. SWE also reduced the expression of inducible NO synthase and cyclooxygenase-2 protein, as well as the production of pro-inflammatory cytokines (such as tumor necrosis factor-α). The phosphorylation levels of the mitogen-activated protein kinase family members, such as JNK and p38, were also reduced by SWE. Thus, SWE could be used as a potential anti-inflammatory agent.
Keywords
anti-inflammatory; Scutellaria baicalensis; RAW 264.7;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Park JS, Kim MH. Anti-inflammatory effects of ricd bran ethanol extract in murine macrophage raw 264.7 cells. Yakhak Hoeji 55: 451-461 (2011)
2 Park JW, Kwon OK, Ryu HW, Paik JH, Paryanto I, Yuniato P, Choi SH, Oh SR, Ahn KS. Anti-inflammatory effects of Passiflora foetida L. in LPS-stimulated RAW264.7 macrophages. Int. J. Mol. Med. 41: 3709-3716 (2018)
3 Wang ZL, Wang S, Kuang Y, Hu ZM, Qiao X, Ye M. A comprehensive review on phytochemistry, pharmacology, and flavonoid biosynthesis of Scutellaria baicalensis. Pharm. Biol. 56: 465-484 (2018)   DOI
4 Qing Z, Chen XY, Martin C. Scutellaria baicalensis, the golden herb from the garden of Chinese medicinal plants. Sci. Bull. 61: 1391-1398 (2016)   DOI
5 Zhao T, Tang H, Xie L, Zheng Y, Ma Z, Sun Q, Li X. Scutellaria baicalensis Georgi. (Lamiaceae): a review of its traditional uses, botany, phytochemistry, pharmacology and toxicology. J. Pharm. Pharmacol. 71: 1353-1369 (2019)   DOI
6 Iontcheva I, Amar S, Zawawi KH, Kantarci A, Van Dyke TE. Role for moesin in lipopolysaccharide-stimulated signal transduction. Infect. Immun. 72: 2312-2320 (2004)   DOI
7 Kuo SW, Su WL, Chou TC. Baicalin improves the survival in endo-toxic mice and inhibits the inflammatory responses in LPS-treated RAW 264.7 macrophages. Eur. J. Inflamm. 18: 1-12 (2020)
8 Jung JY, Lee JR, Byun SH, Jung JW, Kim YH, Kim SC. Inhibitory effect of Dioscorea bulbifera MeOH extract on pro-inflammatory mediator In vitro and In vivo. J. Physiol. Path. Kor. Med. 24: 310-318 (2010)
9 Kang SW. Role of Reactive Oxygen Species in Cell Death Pathways. Hanyang Medical Reviews 33: 77-82 (2013)   DOI
10 Kim TY. Effect of Gagam-Dangueumja through regulation of MAPK on LPS-induced inflammation in Raw 264.7 cells. Kor. J. Orient. Intern. Med. 34: 339-348 (2013)
11 Li Y, Wu Q, Deng Y, Lv H, Qiu J, Chi G, Feng H. D(-)-Salicin inhibits the LPS-induced inflammation in RAW264.7 cells and mouse models. Int. Immunopharmacol. 26: 286-94 (2015)   DOI
12 Kim MS, Jeong J, Lee HY, Ju YS, Bae GS, Seo SW, Cho IJ, Park SJ, Song HJ. The anti-inflammatory effect of Achyranthes japonica on lipopolysaccharide-induced inflammatory activity in murine macrophages. Kor. J. Herbology. 26: 51-57 (2011)   DOI
13 Kim MK, Kim DY. Anti-inflammatory effect of barley leaf ethanol extract in LPS-stimulated RAW264.7 macrophage. Kor. J. Food Preserv. 22: 735-743 (2015)   DOI
14 Lee AK, Sung SH, Kim YC, Kim SG. Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-α and COX-2 expresstion by sauchinone effects on I-Ba phosphorylation, C/EBP and AP-1 activation. Br. J. Pharmacol. 139: 11-20 (2003)   DOI
15 Park HJ, Kim SM, Kwon HJ, Lee HT, Kim BW, Kim TH, Kim MM. Anti-inflammatory effect of Scutellaria baicalensis hot water extracts containing baicalin on modulation of the immune system in Raw264. 7 cells. J. Life Sci. 24: 219-226 (2014)   DOI
16 Matthay MA, Zimmerman GA, Esmon C, Bhattacharya J, Coller B, Doerschuk CM, Floros J, Gimbrone Jr MA, Hoffman E, Hubmayr RD, Leppert M, Matalon S, Munford R, Parsons P, Slutsky AS, Tracey KJ, Ward P, Gail DB, Harabin A. Future research directions in acute lung injury: summary of a National Heart, Lung, and Blood Institute working group. Am. J. Respir. Crit. Care Med. 167: 1027-1035 (2003)   DOI
17 Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 65: 55-63 (1983)   DOI