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http://dx.doi.org/10.9721/KJFST.2020.52.5.516

Physicochemical properties of supercritical carbon dioxide defatted mealworm (Tenebrio molitor) powder and protein isolate  

Kim, Yangji (Department of Food and Nutrition, Dongduk Women's University)
Kim, Seok Joong (Department of Food and Nutrition, Dongduk Women's University)
Publication Information
Korean Journal of Food Science and Technology / v.52, no.5, 2020 , pp. 516-523 More about this Journal
Abstract
Supercritical carbon dioxide (SCO2) extraction was applied for the defatting of mealworm to prepare defatted powder (DP) and protein isolate (PI) and compare the process to press and hexane extraction, with respect to DP and PI physicochemical properties. SCO2 DP was obtained by extracting 34.40% oil at 41.37 MPa, 40℃ for 180 min, and the product contained 71.66% crude protein, which is similar to that of hexane DP and higher than that of press DP. In using alkali protein extraction to prepare PI from DP, SCO2 was as effective as hexane and better than press. SCO2 produced brighter DP and PI than press, but not as much as hexane. Protein solubility was similar in all DP, with minimum values at pH 5. The highest water adsorption capacity was noticeable for SCO2 PI, and SCO2 DP showed an oil adsorption capacity comparable to that of hexane DP. SCO2 DP and PI had better foaming capacity than press DP and PI and showed superior emulsion activity compared to others.
Keywords
mealworm; supercritical carbon dioxide; defatted powder; protein isolate; physicochemical properties;
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Times Cited By KSCI : 13  (Citation Analysis)
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1 Azzollini D, Derossi A, Fogliano V, Lakemond CMM, Severini C. Effects of formulation and process conditions on microstructure, texture and digestibility of extruded insect-riched snacks. Innov. Food Sci. Emerg. Technol. 45: 344-353 (2018)   DOI
2 Baek M, Hwang J-S, Kim MA, Kim S-H, Goo T-W, Yun EY. Comparative analysis of nutritional components of edible insects registered as novel foods. J. Life Sci. 27: 334-338 (2017a)   DOI
3 Baek M, Seo M, Kim M-A, Yun EY, Hwang J-S. The antioxidant activities and hair-growth promotion effects of Tenebrio molitor larvae extracts (TMEs). J. Life Sci. 27: 1269-1275 (2017b)   DOI
4 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal. Biochem. 72: 248-54 (1976)   DOI
5 Kim HW, Setyabrata D, Lee YJ, Jones OG, Kim YHB. Pre-treated mealworm larvae and silkworm pupae as a novel protein ingredient in emulsion sausages. Innov. Food Sci. Emerg. Technol. 38: 116-123 (2016)   DOI
6 Kim SM, An C-W, Han J-A. Characterization and application of the proteins isolated from edible insects. Korean J. Food Sci. Technol. 51: 537-542 (2019a)   DOI
7 Min KT, Kang MS, Kim MJ, Lee SH, Han JS, Kim AJ. Manufacture and quality evaluation of cookies prepared with mealworm (Tenebrio molitor) powder. Korean J. Food Nutr. 29: 12-18 (2016)   DOI
8 Mariod AA, Abdelwahab SI, Gedi MA, Solati Z. Supercritical carbon dioxide extraction of sorghum bug (Agonoscelis pubescens) oil using response surface methodology. J. Am. Oil Chem. Soc. 87: 849-856 (2010)   DOI
9 Martnez J, De Aguia AC. Extraction of triacylglycerols and fatty acids using supercritical fluids-Review. Curr. Anal. Chem. 10: 67-77 (2014)   DOI
10 Mattia CD, Battista N, Sacchetti G, Serafini M. Antioxidant activities in vitro of water and liposoluble extracts obtained by different species of edible insects and invertebrates. Front. Nutr. 6: 106 (2019)   DOI
11 Ministry of Food and Drug Safety. Korea food code. Available from: http://www.nifds.go.kr/brd/m_21/view.do?seq=7819&srchFr=&srchTo=&srchWord=&srchTp=&itm_seq_1=0&itm_seq_2=0&multi_itm_seq=0&company_cd=&company_nm=&page=99. Accessed Feb. 19 (2016)
12 Finke MD. Complete nutrient composition of commercially raised invertebrates used as food for insectivores. Zoo Biol. 21: 269-285 (2002)   DOI
13 BuBler S, Rumpold BA, Jander E, Rawel HM, Schlter OK. Recovery and techno- functionality of flours and proteins from two edible insect species: Mealworm (Tenebrio molitor) and black soldier fly (Hermetia illucens) larvae. Heliyon 2: e00218 (2016)   DOI
14 Cito A, Dreassi E, Frosinini R, Zanfini A, Pianigiani C, Botta M, Francardi V. The potential beneficial effects of Tenebrio molitor (Coleoptera tenebrionidae) and Galleria mellonella (Lepidoptera pyralidae) on human health. REDIA 100: 125-133 (2017)
15 El Nasri ENA, El Tinay AH. Functional properties of fenugreek (Trigonella foenum graecum) protein concentrate. Food Chem. 103: 582-589 (2007)   DOI
16 Finke MD. Estimate of chitin in raw whole insects. Zoo Biol. 26: 105-115 (2007)   DOI
17 Godfray HCJ, Crute IR, Haddad L, Lawrence D, Muir JF, Nisbett N, Pretty J, Robinson S, Toulmin C, Whiteley R. The future of the global food system. Philos. Trans. R. Soc. B-Biol. Sci. 365: 2769-2777 (2010)   DOI
18 Halling PJ. Protein stabilized foams and emulsions. Crit. Rev. Food Sci. Nutr. 15: 155-203 (1981)   DOI
19 Jones LD, Cooper RW, Harding RS. Composition of mealworm Tenebrio molitor larvae. J. Zoo Anim. Med. 3: 34-41 (1972)
20 Sreerama YN, Sashikala VB, Pratape VM, Singh V. Nutrients and antinutrients in cowpea and horse gram flours in comparison to chickpea flour: Evaluation of their flour functionality. Food Chem. 131: 462-468 (2012)   DOI
21 Lee KH, Yoon YT, Park YI, Lee HJ, Jeong NY. Quality evaluation of acorn mook prepared with mealworm (Tenebrio molitor) powder. J. Korean Soc. Food Sci. Nutr. 30: 1042-1047 (2017)
22 Kim Y, Imm J-Y, Kim I-H, Kim SJ. Functional and emulsifying properties of balloonflower seeds oil extracted by supercritical carbon dioxide. J. Korean App. Sci. Technol. 36: 226-236 (2019b)   DOI
23 Kim Y, Woo H, Imm J-Y, Kim SJ. Evaluation of nutritional characteristics of Platycodon grandiflorum seeds. J. Oil Appl. Sci. 35: 478-484 (2018)   DOI
24 Kim YM. Quality characteristics of white bread with hot air-dried Tenebrio molitor larvae Linne powder. Korean J. Food Cook. Sci. 33: 513-522 (2017)   DOI
25 Mæhre HK, Dalheim L, Edvinsen GK, Elvevoll EO, Jensen I-J. Protein determination-method matters. Foods 7: 5 (2018)   DOI
26 Wani IA, Sogi DS, Wani AA, Gill BS. Physico-chemical and functional properties of flours from Indian kidney bean (Phaseolus vulgaris L.) cultivars. LWT-Food Sci. Technol. 53: 278-284 (2013)   DOI
27 Nowak V, Persijn D, Rittenschober D, Charrondiere UR. Review of food composition data for edible insects. Food Chem. 193: 39-46 (2016)   DOI
28 Omana DA, Moayedi V, Xu Y, Betti M. Alkali-aided protein extraction from chicken dark meat: Textural properties and color characteristics of recovered proteins. Poult. Sci. 89: 1056-1064 (2010)   DOI
29 Paredes-Lopez O, Guevara-Lara F, Schevenin-Pinedo ML, Montes-Rivera R. Comparison of procedures to determine protein content of developing bean seeds (Phaseolus vulgaris). Plant Food Hum. Nutr. 39: 137-148 (1989)   DOI
30 Van Huis A, Van Itterbeeck J, Klunder H, Mertens E, Halloran A, Muir G, Vantomme P. Edible insects. Future prospects for food and feed security. FAO, Rome, Italy. pp 201 (2013)
31 Woo J, Lee H, Choi J, Moon K. Quality characteristics of tea of Tenebrio molitor larvae according to manufacturing methods. Korean J. Food Preserv. 26: 179-184 (2019)   DOI
32 Yi L, Van Boekel MAJS. Lakemond CMM. Extracting Tenebrio molitor protein while preventing browning: effect of pH and NaCl on protein yield. J. Insects as Food Feed 3: 21-31 (2017)   DOI
33 Yu M-H, Lee H-S, Cho H-R, Lee S-O. Enzymatic preparation and antioxidant activities of protein hydrolysates from Tenebrio molitor larvae (mealworm). J. Korean Soc. Food Sci. Nutr. 46: 435-441 (2017)   DOI
34 Zhao X, Vzquez-Gutirrez JL, Johansson DP, Landberg R, Langton M. Yellow mealworm protein for food purposes-Extraction and functional properties. PLoS ONE 11: e0149991 (2016)   DOI
35 Zieliska E, Baraniak B, Kara M, Rybczyska K, Jakubczyk A. Selected species of edible insects as a source of nutrient composition. Food Res. Int. 77: 460-466 (2015)   DOI
36 Zieliska E, Kara M, Baraniak B. Comparison of functional properties of edible insects and protein preparations thereof. LWT-Food Sci. Technol. 91: 168-174 (2018)   DOI
37 Kou imska L, Adamkova A. Nutritional and sensory quality of edible insects. NFS J. 4: 22-26 (2016)   DOI
38 Roy BC, Sasaki M, Goto M. Effect of temperature and pressure on the extraction yield of oil from sunflower seed with supercritical carbon dioxide. J. Appl. Sci. 6: 71-75 (2006)   DOI
39 Purschke B, Stegmann T, Schreiner M, Jger H. Pilot-scale supercritical $CO_2$ extraction of edible insect oil from Tenebrio molitor L. larvae-Influence of extraction conditions on kinetics, defatting performance and compositional properties. Eur. J. Lipid Sci. Tech. 119: 1600134 (2016)   DOI
40 Ravzanaadii N, Kim SH, Choi WH, Hong SJ, Kim NJ. Nutritional value of mealworm, Tenebrio molitor as food source. Int. J. Indust. Entomol. 25: 93-98 (2012)   DOI
41 Rumpold BA, Schlter OK. Nutritional composition and safety aspects of edible insects. Mol. Nutr. Food Res. 57: 802-823 (2013)   DOI
42 Sathe SK, Deshpande SS, Salunkhe DK. Functional properties of winged bean (Psophocarpus tetragonolobus, L.) proteins. J. Food Sci. 47: 503-508 (1982)   DOI
43 Sathe SK, Salunkhe DK. Functional properties of the great northern bean (Phaseolus vulgaris L.) proteins: Emulsion, foaming, viscosity and gelation properties. J. Food Sci. 46: 71-74 (1981)   DOI
44 Son Y-J, Hwang J-Y. Physicochemical characteristics and oxidative stabilities of defatted mealworm powders under different manufacturing conditions. J. East Asian Soc. Diet. Life 27: 194-203 (2017)   DOI