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http://dx.doi.org/10.9721/KJFST.2020.52.5.441

Inhibitory effect of Allium macrostemon extracts on adipogenesis of 3T3-L1 preadipocytes  

Lee, Joo-Yeon (Department of Food and Nutrition, Yeungnam University)
Jeong, Yeju (Department of Food and Nutrition, Yeungnam University)
Kim, Jina (Department of Food and Nutrition, Yeungnam University)
Kim, Choon Young (Department of Food and Nutrition, Yeungnam University)
Publication Information
Korean Journal of Food Science and Technology / v.52, no.5, 2020 , pp. 441-449 More about this Journal
Abstract
The aim of this study was to compare the biological activities of whole-plant (WAE), bulb (BAE), and leaf (LAE) extracts of Allium macrostemon. The antioxidant activities, total polyphenol contents, and anti-adipogenic activities of WAE and LAE were superior to those of BAE, whereas the biological effects of WAE and LAE were similar. Therefore, the effect of LAE on adipogenesis was further investigated. Treatment of preadipocytes with LAE at 100 g/mL resulted in the inhibition of intracellular lipid accumulation by 49.64%. Consistent with this result, quantitative reverse transcription-PCR showed that LAE treatment decreased the gene expressions of CCAAT/enhancer-binding protein beta (C/EBPβ), peroxisome proliferator-activated receptor gamma (PPARγ), C/EBPα and stearoyl-CoA desaturase 1 (SCD1). Thus, LAE attenuates the adipogenesis of preadipocytes by suppressing the expression of adipogenic and lipogenic genes. These results suggest that LAE can be potentially useful as a functional ingredient to prevent obesity in the food industry.
Keywords
anti-obesity; adipocyte differentiation; Allium macrostemon; long-stamen chive; antioxidant activity;
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1 Chang E, Kim CY. Natural products and obesity: a focus on the regulation of mitotic clonal expansion during adipogenesis. Molecules. 24: 1157 (2019)   DOI
2 Choe SS, Huh JY, Hwang IJ, Kim JI, Kim JB. Adipose Tissue Remodeling: Its Role in Energy Metabolism and Metabolic Disorders. Front. Endocrinol. 7: 30 (2016)
3 Cignarelli A, Genchi VA, Perrini S, Natalicchio A, Laviola L, Giorgino F. Insulin and insulin receptors in adipose tissue development. Int. J. Mol. Sci. 20: 759 (2019)   DOI
4 Farmer, SR. Transcriptional control of adipocyte formation. Cell Metab. 4: 263-273 (2006)   DOI
5 Guo D, Bell EH, Mischel P, Chakravarti, A. Targeting SREBP-1-driven lipid metabolism to treat cancer. Curr. Pharm. Des. 20: 2619-2626 (2014)   DOI
6 Morrison RF, Farmer SR. Hormonal signaling and transcriptional control of adipocyte differentiation. J. Nutr. 130: 3116S-3121S (2000)   DOI
7 Muni Swamy G, Ramesh G, Devi Prasad R, Meriga B. Astragalin,(3-O-glucoside of kaempferol), isolated from Moringa oleifera leaves modulates leptin, adiponectin secretion and inhibits adipogenesis in 3T3-L1 adipocytes. Arch. Physiol. Biochem. 1-7 (2020)
8 Im JS, Lee EH, Lee JN, Kim KD, Kim HY, Kim MJ. Sulforaphane and Total Phenolics Contents and Antioxidant Activity of Radish according to Genotype and Cultivation Location with Different Altitudes. HST. 28: 335-342 (2010)
9 Hamissa AMB, Seffen M, Aliakbarian B, Casazza AA, Perego P, Converti A. Phenolics extraction from Agave americana (L.) leaves using high-temperature, high-pressure reactor. Food Bioprod. Process. 90: 17-21 (2012)   DOI
10 Han IH, Kim J-H. Antioxidant and Physiological Activities of Water and Ethanol Extracts of Diverse Parts of Welsh Onion. J. Korean Soc. Food Sci. Nutr. 46: 426-434 (2017)   DOI
11 Jung JE, Sin SM, Kim HM, Lee S, Choi K, Park KW, Ku JJ, Cho EJ. Screening of radical scavenging activity and gastric cancer prevention activity from Korean folk plants. J. Cancer Prev. 16: 65-73 (2011)
12 Kim HJ, Lee M-J, Jang J-Y, Lee S-H. Allium hookeri Root Extract Inhibits Adipogenesis by Promoting Lipolysis in High Fat DietInduced Obese Mice. Nutrients. 11: 2262 (2019)   DOI
13 World Health Organization (WHO). Obesity. Available from: https://www.who.int/news-room/facts-in-pictures/detail/6-facts-on-obesity. Accessed Feb. 10, 2020.
14 Nakane R, Iwashina T. Flavonol glycosides from the leaves of Allium macrostemon. Nat. Prod. Commun. 10: 1934578X1501000817 (2015)
15 Singleton VL, Orthofer R, Lamuela-Ravents RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Meth. Enzymol. 299: 152-178 (1999)   DOI
16 Wang X, Hai C. Redox modulation of adipocyte differentiation: hypothesis of "Redox Chain" and novel insights into intervention of adipogenesis and obesity. Free Radic. Biol. Med. 89: 99-125 (2015)   DOI
17 Xie W, Zhang Y, Wang N, Zhou H, Du L, Ma X, Shi X, Cai G. Novel effects of macrostemonoside A, a compound from Allium macrostemon Bung, on hyperglycemia, hyperlipidemia, and visceral obesity in high-fat diet-fed C57BL/6 mice. Eur. J. Pharmacol. 599: 159-165 (2008)   DOI
18 Yao D, Luo J, He Q, Shi H, Li J, Wang H, Xu H, Chen Z, Yi Y, Loor J J. SCD1 alters longchain fatty acid (LCFA) composition and its expression is directly regulated by SREBP1 and $PPAR{\gamma}$ 1 in dairy goat mammary cells. J. Cell. Physiol. 232: 635-649 (2017)   DOI
19 Zhang Z, Wang F, Wang M, Ma L, Ye H, Zeng XJ. A comparative study of the neutral and acidic polysaccharides from Allium macrostemon Bunge. Carbohydr. Polym. 117: 980-987 (2015)   DOI
20 Balogun O, Pei Y, Kang HW. Garlic Scape Extract Decreases Adipogenesis and Lipogenesis by Activating AMK-Activated Protein Kinase in 3T3-L1 Adipocytes. CDN. 4: 1608-1608 (2020)
21 Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal. Biochem. 239: 70-76 (1996)   DOI
22 Brand-Williams W, Cuvelier M-E, Berset CLWT. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol. 28: 25-30 (1995)   DOI
23 Lee CW, Seo JY, Lee J, Choi JW, Cho S, Bae JY, Sohng JK, Kim SO, Kim J, Park YI. 3-O-Glucosylation of quercetin enhances inhibitory effects on the adipocyte differentiation and lipogenesis. Biomed. Pharmacother. 95: 589-598 (2017)   DOI
24 Kim K-H, Kim H-J, Byun M-W, Yook H-S. Antioxidant and Antimicrobial Activities of Ethanol Extract from Six Vegetables Containing Different Sulfur Compounds. J. Korean Soc. Food Sci. Nutr. 41: 577-583 (2012)   DOI
25 Kim NE, Seo DY, Cheon WY, Choi YM, Lee JS, Kim YH. Bioactive Nutritional Compounds and Antioxidant Activity of Green Peppers Consumed in Korea. J. Korean Soc. Food Sci. Nutr. 49: 141-148 (2020)   DOI
26 Kim Y-H, Shon M-Y, Sung N-J. Antioxidant and Antimutagenic Activities of Hot Water Extract from White and Yellow Onions after Simulated Gastric Digestion. J. Life Sci. 14: 925-930 (2004)   DOI
27 Korean Society for the Study of Obesity (KSSO). 2019 Obesity Fact Sheet. Obesity Fact Sheet. 5: in press (2019)
28 Ksouri R, Megdiche W, Debez A, Falleh H, Grignon C, Abdelly C. Salinity effects on polyphenol content and antioxidant activities in leaves of the halophyte Cakile maritima. Plant Physiol. Biochem. 45: 244-249 (2007)   DOI
29 Lowe CE, O'Rahilly S, Rochford JJ. Adipogenesis at a glance. J. Cell. Sci. 124: 2681-2686 (2011)   DOI
30 Makino T, Kanemaru M, Okuyama S, Shimizu R, Tanaka H, Mizukami H. Anti-allergic effects of enzymatically modified isoquercitrin ($\alpha$-oligoglucosyl quercetin 3-O-glucoside), quercetin 3-O-glucoside, $\alpha$-oligoglucosyl rutin, and quercetin, when administered orally to mice. J. Nat. Med. 67: 881-886 (2013)   DOI
31 Manach C, Scalbert A, Morand C, Rmsy C, Jimnez L. Polyphenols: food sources and bioavailability. Am J Clin Nutr. 79: 727-747 (2004)   DOI
32 Moreno-Navarrete JM, Fernndez-Real JM. Adipocyte Differentiation. Adipose Tissue Biology. 69-90 (2017)
33 Zhang Z, Wang F, Wang M, Ma L, Zeng X. Extraction optimisation and antioxidant activities in vitro of polysaccharides from Allium macrostemon Bunge. Int. J. Food Sci. 47: 723-730 (2012)   DOI